Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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Determination of Flavonoids from Allium victorialis var. platyphyllum and Their Effect on Gap Junctional Intercellular Communication

  • Hong, Eun-Young (Department of Food and Nutrition, Duksung Women's University) ;
  • Choi, Soo-Im (Plant Resources Research institute, Duksung Women's University) ;
  • Kim, Gun-Hee (Department of Food and Nutrition, Duksung Women's University)
  • Published : 2007.10.31
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Abstract

This study was carried out to identify and quantify the flavonoids from 6 different plant parts of Allium victorialis var. platyphyllum (AVP), including the flower, leaf, root, stem, flower stalk, and flower seed, using liquid chromatography/ mass spectrometry. Two major flavonoids were structurally identified as quercetin (3,5,7,3'4,'-pentahydroxyflavone) and kaempferol (3,5,7,4'-tetrahydroxyflavone) at contents of 11.8-25.8 and $6.0-64.4\;{\mu}g/mL$, respectively. In particular, the flower and root plant parts contained the highest amounts of quercetin and kaempferol compared to the other parts. We also assessed the recovery effects of each plant-part extract of AVP on gap junctional intercellular communication (GJIC) in WB-F344 cells by the scrape-loading and dye transfer (SL/DT) method. According to the results, GJIC was reduced by approximately 70.2% ($62.3{\pm}12.5$ cells) compared to the control ($209{\pm}9.5$ cells, 100%) when 12-O-tetradecanoylphorbol-13-acetate (TPA) was treated alone in the WB-F344 rat liver epithelial cells. However, the stem extract (0.2 mg/mL) restored GJIC to basal levels (92%, $204{\pm}2.3$ cells, p<0.01) and the flower extract (0.2 mg/mL) stimulated GJIC to 82.5% ($172.6{\pm}8.3$ cells, p<0.05), when applied together with the TPA.

Keywords

References

  1. Lim SC, Park HJ, Yun SY, Lee MS, Kim WB, Jung WT. Structures of flavonoids and furostanol glycosides isolated from the bulbs of Allium victorialis L. J. Korean Soc. Hort. Sci. 37: 675-679 (1996)
  2. Moon KS. Usage and Components of Herbal Medicine. Scientific Encyclopedia Co., Pyongyang, Korea. pp. 671-672 (1984)
  3. Choi JW, Lee KT, Kim WB, Park KG, Jung HJ, Park HJ. Pharmacological effects of the Allium victorialis var. platyphyllum extracts on the rats induced by streptozotocin, poloxamer-407, $CCl_4$, and D-galactosamine. Korean J. Pharmacogn. 34: 250-255 (2003)
  4. Lawson LD, Wang ZJ, Hughes BG. $\gamma$-Glutamyl-S-alkylcysteines in garlic and other Allium spp.: Precursors of age-dependent trans-lpropenyl thiosulfinates. J. Nat. Prod. 54: 436-444 (1991) https://doi.org/10.1021/np50074a014
  5. Plessi M, Bertelli D, Rastelli G, Albasini A, Monzani A. Fruits of ribose, rubus, vaccinium, and prenus genus. Metal contents and genome, Fresenius. J. Anal. Chem. 361: 353-354 (1998) https://doi.org/10.1007/s002160050902
  6. Robards K, Antolovich M. Analytical chemistry of fruit bioflavonoids. Analyst 122: 11R-34R (1997) https://doi.org/10.1039/a606499j
  7. Hertog MGL, Hollman PCH, Katan MB. Content of potentially anticarcinogenic flavonoids of 28 vegetables and 9 fruits commonly consumed in the Netherlands. J. Agr. Food Chem. 40: 2379-2383 (1992) https://doi.org/10.1021/jf00024a011
  8. Bruzzone R, White TW, Paul DL. Connections with connexins: The molecular basis of direct intercellular signaling. Eur. J. Biochem. 238: 1-27 (1996) https://doi.org/10.1111/j.1432-1033.1996.0001q.x
  9. Kumar NM, Gilula NB. The gap junction communication channel. Cell 84: 381-388 (1996) https://doi.org/10.1016/S0092-8674(00)81282-9
  10. Loewenstein WR, Kanno Y. Intercellular communication and the control of tissue growth: Lack of communication between cancer cells. Nature 209: 1248-1249 (1996) https://doi.org/10.1038/2091248a0
  11. Fitzgerald DJ, Yamasaki H. Tumor promotion: Models and assay systems. Teratogen. Carcin. Mut. 10: 89-102 (1990) https://doi.org/10.1002/tcm.1770100205
  12. Yotti LP, Chang CC, Trosko JE. Elimination of metabolic cooperation in Chineses hamster cells by a tumor promoter. Science 206: 1089-1091 (1979) https://doi.org/10.1126/science.493994
  13. Trosko JE, Ruch RJ. Cell-cell communication in carcinogenesis. Front. Biosci. 3: D208-D236 (1998) https://doi.org/10.2741/A275
  14. Bertram JS, Pung A, Churley M, Kappock TJ, Wilkins LR, Cooney RV. Diverse carotenoids protect against chemically induced neoplastic transformation. Carcinogenesis 12: 671-678 (1991) https://doi.org/10.1093/carcin/12.4.671
  15. Upham BL, Kang KS, Cho HY, Trosko JE. Hydrogen peroxide inhibits gap junctional intercellular communication in glutathione sufficient but not glutathione deficient cells. Carcinogenesis 18: 37- 42 (1997) https://doi.org/10.1093/carcin/18.1.37
  16. Sharov VS, Briviba K, Sies H. Peroxynitrite diminishes gap junctional communication: Protection by selenite supplementation. IUBMB Life 48: 379-384 (1999) https://doi.org/10.1080/713803538
  17. Chung HK, Choi CS, Park WJ, Kang MH. Radical scavenging activity of grape-seed extracts prepared from different solvents. Food Sci. Biotechnol. 14: 715-721 (2005)
  18. Choi YM, Ku JB, Chang HB, Lee JS. Antioxidant activities and total phenolics of ethanol extracts from several edible mushrooms produced in Korea. Food Sci. Biotechnol. 14: 700-703 (2005)
  19. Jean M, Emilien P, Karl GL, Jean L, Salwa K, Selim K. Quantification of phenolic contents and antioxidant capacity of Atlantic sea cucumber, Cucumaria frondosa. Food Chem. 104: 1040-1047 (2007) https://doi.org/10.1016/j.foodchem.2007.01.016
  20. Lin-Chin YR, Huang CY, Wen KC. Evaluation of quantitative analysis of flavonoid aglycones in Gingko biloba extract and its products. J. Food Drug Anal. 8: 289-296 (2000)
  21. Choi JH, Nam JO, Kim JY, Kim JM, Paik HD, Kim CH. Antioxidant, antimicrobial, and antitumor activities of partially purified substance(s) from green tea seed. Food Sci. Biotechnol. 15: 672-676 (2006)
  22. Jong HC, Cho SD, Hu H, Kim SH, Lee SK, Lee YS, Kang KS. The roles of ERK1/2 and p38 MAP kinases in the preventive mechanisms of mushroom Phellinus linteus against the inhibition of gap junctional intercellular communication by hydrogen peroxide. Carcinogenesis 23: 1163-1169 (2002) https://doi.org/10.1093/carcin/23.7.1163
  23. Rice-Evans CA, Miller NJ. Antioxidnat activities of flavonoids as bioactive components of food. Biochem. Soc. T. 24: 790-795 (1996) https://doi.org/10.1042/bst0240790
  24. Hertog MGL, Hollman PCH, Katan MB. Content of potentially anticarcinogenic flavonoids of 28 vegetables and 9 fruits commonly consumed in the Netherlands. J. Agr. Food Chem. 40: 2379-2383 (1992) https://doi.org/10.1021/jf00024a011
  25. Sibel K, Sedef-Nehir EL. Quercetin, luteolin, apigenin, and kaempferol contents of some foods. Food Chem. 66: 289-292 (1999) https://doi.org/10.1016/S0308-8146(99)00049-7
  26. Sai K, Upham BL, Kang KS, Hasegawa R, Inoue T, Trosko JE. Inhibitory effect of pentachlorophenol on gap junctional intercellular communication in rat liver epithelial cells in vitro. Cancer Lett. 130: 9-17 (1980) https://doi.org/10.1016/S0304-3835(98)00082-2
  27. Nomata K, Kang KS, Hazashi T, Matesic D, Lockwood L, Chang CC, Trosko JE. Inhibition of gap junctional intercellular communication in gepacholr-and heptachlorepoxide-treated normal human breast epithelial cell. Cell Biol. Toxicol. 12: 69-78 (1996) https://doi.org/10.1007/BF00143357
  28. Kang KS, Chang CC, Torsko JE. Modulation of gap junction intercellular communication during human breast stem cell differentiation and immortalization. pp. 347-351. In: Gap Junction. Wener R (ed). IOS Press, Washington, DC, USA (1998)
  29. Budunova IV. Alteration of gap junctional intercellular communication during carcinogenesis. Cancer J. 7: 228-237 (1994)
  30. Yamasaki H. Role of disrupted gap junctional intercellular communication in detection and characterization of carcinogens. Mutat. Res. 465: 91-105 (1996)
  31. Mette N, Randall JR, Ole V. Resveratrol reverses tumor-promoterinduced inhibition of gap-junctional intercellular communication. Biochem. Bioph. Res. Co. 275: 804-809 (2000) https://doi.org/10.1006/bbrc.2000.3378
  32. Chaumontet C, Droumaguet C, Bex V, Heberden C, Gaillard- Sanchez I, Martel P. Flavonoids (apigenin, tangeretin) counteracts tumor promoter-induced inhibition of intercellular communication of rat liver epithelial cells. Cancer Lett. 114: 207-210 (1997) https://doi.org/10.1016/S0304-3835(97)04664-8
  33. Ale-Agha N, Stahl W, Sies H. (-)-Epicatechin effects in rat liver epithelial cells: Stimulation of gap junctional communication and counteraction of its loss due to the tumor promoter 12-O-tetradecanoylphobol- 13-acetate. Biochem. Pharmacol. 63: 2145-2149 (2002) https://doi.org/10.1016/S0006-2952(02)01021-3