Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Comparison of a new ELISA with other serodiagnostic tests for bovine brucellosis

소 브루셀라병의 혈청학적 진단법 비교실험

  • Hur, Jin (Department of Public Health, College of Veterinary Medicine, Chonbuk National University) ;
  • Kakoma, Ibulaimu (Department of Pathobiology, College of Veterinary Medicine, University of Illinois) ;
  • Jeong, Jae-Myong (Jeonbuk Institute of Livestock & Veterinary Research) ;
  • Lee, Hyeon-Jin (Department of Public Health, College of Veterinary Medicine, Chonbuk National University) ;
  • Baek, Byeong-Kirl (Department of Public Health, College of Veterinary Medicine, Chonbuk National University)
  • 허진 (전북대학교 수의과대학) ;
  • ;
  • 정재명 (전라북도 축산위생연구소) ;
  • 이현진 (전북대학교 수의과대학) ;
  • 백병걸 (전북대학교 수의과대학)
  • Published : 2007.09.30
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Abstract

A novel enzyme linked immunosorbent assay (ELISA) is described and compared with other established serologic tests for bovine brucellosis, namely the rose bengal test (RBT), the complement fixation test (CFT), and the tube agglutination test (TAT) approved and used in Korea. A total of 109 bovine serum samples were tested using all the 4 assays and analyzed as to specificity, sensitivity, reproducibility and predictive value. The ELISA showed 100% agreement with the CFT. The least agreement between ELISA was observed with the TAT. The agreement between the ELISA and RBT was not significantly different from that observed between the CFT and the ELISA. It is concluded that the new assay would be a good candidate for routine serologic survey for brucellosis in Korea. A protocol combining the ELISA and the CFT would increase the power for detection of serologically positive individuals and herds.

Keywords

References

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