경북지역 애견 번식장에서 분리한 Brucella canis의 생화학적특성 및 PFGE 양상

Biochemical characterization and PFGE pattern of Brucella canis isolated from kennels in Gyoengbuk province

  • 김성국 (경상북도 가축위생시험소) ;
  • 김영환 (경상북도 가축위생시험소) ;
  • 홍현표 (경상북도 가축위생시험소) ;
  • 엄현정 (경상북도 가축위생시험소) ;
  • 장성준 (경상북도 가축위생시험소) ;
  • 조민희 (경상북도 가축위생시험소) ;
  • 이양수 (경상북도 가축위생시험소)
  • 발행 : 2007.09.30

초록

A biochemical characterization and antimicrobial drugs susceptibility study was conducted in four breeding kennel which was canine abortion caused by Brucella canis in Gyeongbuk province in 2003-2006. Total of 267 dogs domesticated in the four kennel were examination. Among them, 143 (53.6%) dogs were sero-positive and 25 of blood samples were isolated to Brucella canis. At amplification of 35KDa-BCSP gene using PCR, 711 bp DNA fragment was same visible in 25 isolates and B canis RM6/66. Biochemical characterization of B canis isolated was non-hemolytic, no production of $H_2S$, no fermentation of carbohydrates, catalase-positive, oxidase-positive, indol-negative, hydrolyzation of urea, reduction of nitrate and development of thionin dye medium. Using disk-diffusion method, all of 25 strains tested were found to be highly susceptible to tetracycline, aminoglycoside, quinolone, macrolide antibiotics, rifampin and ampicillin in vitro. Using PFGE with restriction enzyme Smi I, 25 isolates tested were typed to 2 pattern, S1 and S2.

키워드

참고문헌

  1. Timoney JF, Gillespie JH, Scott FW, et al. 1988. Hagan and Brunner's Microbiology and Infectious Disease of Domestic Animals. 8 eds. Comstock Pub Ass, Ithaca and London:148-152
  2. Beran GW. 1994. Handbook of Zoonosis. 2 eds. CRC Press, London:9-39
  3. Corbel MJ, Brinley-Morgan WJ. 1984. Genus Brucella Meyer and Shaw 1920, 173AL. In: Bergey's Manual of Systematic Bacteriology, Vol 1. Krieg NR, Holt JG, Murray RGE, et al. Eds, Williams & Wilkins, Baltimore and London:377-388
  4. Carter GR, Chengappa MM, Roberts AW, et al. 1995. Essential of Veterinary Microbiology. 5 eds. Williams & Willkins, Baltimore:199-204
  5. Carmichael LE, Bruner DW. 1968. Characteristics of a newly-recognized species of Brucella responsible for infectious canine abortions. Cornell Vet 48(4):579-592
  6. Carmichael LE, Shin SJ. 1996. Canine brucellosis: a diagnostician's dilemma. Semin Vet Med Surg (Small Anim) 11(3):161-165 https://doi.org/10.1016/S1096-2867(96)80028-4
  7. Carmichael LE, Kenney RM. 1970. Canine brucellosis: The clinical disease, pathogenesis, and immune response. JAVMA 156(12):1726-1734
  8. Spink WW. 1970. Comments on canine brucellosis due to Brucella canis. JAVMA 156(12):1734-1736
  9. Morisset R, Spink WW. 1969. Epidemic canine brucellosis due to a new species, Brucella canis. Lancet 2(7628):1000‑1002
  10. Moore JA, Gupta BN. 1970, Epizootiology, diagnosis, and control of Brucella canis. JAVMA 156(12):1737-1740
  11. Pickerill PA. 1970. Comments on epizootiology and control of canine brucellosis. JAVMA 156(12):1741-1742
  12. Carmichael LE, Kenney RM. 1968. Canine abortion caused by Brucella canis. JAVMA 152(6):605-616
  13. Jian H. 1992. Identification and characterization of 200 strains of Brucella canis under test from China. Wei Sheng Wu Xue Bao 32(5):370-375
  14. Tak RB, Chun DK. 1972. Isolation of Brucella suis from aborted fetus of a dog. J Kor Soc Microbiol 7(1):17-20
  15. 박용호, 강승원, 주이석 등. 1984. 개 브루셀라 진단액 생산 기초시험 및 항체조사. 가축위생연구소 시험보고:94-98
  16. 문진산, 오기석, 박인철 등. 1999. 전남지방의 소형견 번식장으로부터 발생한 canine brucellosis. 대한수의학회지 39(6):1099‑1105
  17. 박청규, 오지연. 2001. 대구지역 개의 Brucella canis 감염에 대한 세균학적 및 혈청학적 조사. 대한수의학회지 41(1):67-71
  18. Chang CH, Lee JC, Lee CY, et al. 2000. Canine brucellosis in the Jindo. Korean J Vet Clin Med 17(2):321-326
  19. 김종완, 이영주. 2003. 집단 개사육농장에서의 canine brucellosis 발생 및 PCR‑RFLP를 이용한 분리주의 특성조사. 대한수의학회지 43(1):67-75
  20. Alton GG, Jones LM, 1975. Laboratory Techniques in brucellosis. 2 eds. Geneva. WHO:149-156
  21. 정석찬, 정병열, 우승룡 등. 1998. 종모우 정액중 Brucella균 신속 검출을 위한 PCR기법 개발. 대한수의학회지 38(2):345-352
  22. National Committee for Clinical Laboratory Standards. 2000. Performance standards for antimicrobial disk susceptibility test: approved standards. 7 eds. NCCL, Wayne, USA:M100-S10
  23. CDC, Pulsenet. 2004. Standardized laboratory protocol for molecular subtyping of Escherchia coli O157:H7, non-typhoidal Salmonella serotypes, and Shigella sonnei by PFGE. http://www.cdc.gov/pulsenet/protocols/ecoli_salmonella_shigella_protocols.pdf
  24. Ridler AL, Leyland MJ, Fenwick SG, et al. 2005. Demonstration of polymorphism among Brucella ovis field isolates by pulsed-field gel electrophoresis. Vet Microbiol 108(1-2) : 69-74 https://doi.org/10.1016/j.vetmic.2005.02.008
  25. Vizcaino N, Verger JM, Grayon M, et al. 1997. DNA polymorphism at the omp-31 locus of Brucella spp: evidence for a large deletion in Brucella abortus and other species-specific marker. Microbiology 143(Pt 9):2913-2921 https://doi.org/10.1099/00221287-143-9-2913
  26. 김종완, 이영주, 탁연빈. 2003. 국내분리 Brucella canis의 항균제 감수성, 한국임상수의학회지 20(1):86-90
  27. Terakado N, Ueda H, Sugawara H, et al. 1978. Drug susceptibility of Brucella canis isolated from dogs. Nippon Juigaku Zasshi 40(3):291-295 https://doi.org/10.1292/jvms1939.40.291
  28. Olive DM, Bean P. 1999. Principles and applications of methods for DNA-based typing of microbial organisms. J Clin Micbiol 37(6) : 1661-1669
  29. Verger JM, Grimont F, Grimont PA, et al. 1985. Taxonomy of genus Brucella. Ann Ins Pasteur Microbio. 138(2) : 292-295
  30. Jensen AE, Cheville NF, Ewalt DR, et al. 1995. Application of pulsed‒field gel electrophoresis for differentiation of vaccine strain RB51 from field isolates of Brucella abortus from cattle, bison, and elk. Am J Vet Res 56(3) : 308-312