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Establishment of Technology for Preventing the Soybean Sprout Colletotrichum gloeosporioides Rot

열처리에 의한 콩나물 탄저병의 방제

  • Lee, Jung-Han (Department of Applied Biology & Environmental Sciences, Gyeongsang National University) ;
  • Han, Ki-Soo (Department of Applied Biology & Environmental Sciences, Gyeongsang National University) ;
  • Kim, Tae-Hyoung (Department of Applied Biology & Environmental Sciences, Gyeongsang National University) ;
  • Bae, Dong-Won (Central Laboratory, Gyeongsang National University) ;
  • Kim, Dong-Kil (Department of Applied Biology & Environmental Sciences, Gyeongsang National University) ;
  • Kang, Jin-Ho (Department of Agronomy, Gyeongsang National University) ;
  • Kim, Hee-Kyu (Department of Applied Biology & Environmental Sciences, Gyeongsang National University)
  • 이정한 (경상대학교 농업생명과학대학 응용생물환경학과) ;
  • 한기수 (경상대학교 농업생명과학대학 응용생물환경학과) ;
  • 김태형 (경상대학교 농업생명과학대학 응용생물환경학과) ;
  • 배동원 (경상대학교 공동실험실습관) ;
  • 김동길 (경상대학교 농업생명과학대학 응용생물환경학과) ;
  • 강진호 (경상대학교 농업생명과학대학 농학과) ;
  • 김희규 (경상대학교 농업생명과학대학 응용생물환경학과)
  • Published : 2007.08.01

Abstract

Anthracnose fungus was most pathogenic on soybean sprout, of the fungi and bacteria isolated from rotten sprout on market. Bacterial strains associated were not virulent. Dry heat (DHT) applied even as high as $65^{\circ}C$ for 30min. was not effective enough to eliminate the artificially inoculated Colletotrichum gloeosporioides propagules from seedllots. Hot water immersion treatment (HWT), at elevated temperature of $55^{\circ}C$ for 20 min, did eliminate the pathogen but reduced seed germinating and retarded sprout growth: Seed germination was practically acceptable when the seedlots were exposed to at $55^{\circ}C$ for 5 min, but about 20% anthracnose propagules survived. Accordingly, we have optimized the HWT scheme for 5 min at $60^{\circ}C$. This scheme was validated, at small to large scale production system, that surely rule out the possible carry over of the bacterial contaminant from seedlots. This result should improve the shelf-life of soybean sprout on the market.

콩나물 탄저병균 포자에 대한 열처리효과를 조사한 결과 $65^{\circ}C$에 30분까지 건열처리 하였을 때는 균의 사멸효과가 50% 정도에 불과하여, 건열처리는 나물콩 살균에 활용할 수 없었다. 병원균은 $55^{\circ}C$에서 20분 이상, 또는 $60^{\circ}C$에서 5분간 습열처리하면 완전히 사멸하였고, 부생성 세균 사멸효과도 높은 것으로 나타났다. $55^{\circ}C$에서 5분간 습열처리 조건하에서는, 발아장애 및 하배축 기형은 발생하지 않았으나, 20%의 탄저병균 분생포자가 생존하였기 때문에 실용적가치가 인정되지 않았다. 따라서 종자발아 및 하배축 기형이 발생하지 않는 최적 열처리 조건인 $60^{\circ}C$에서 5분간 습열처리를 실제 콩나물 재배 공정모델에 적용한 결과 탄저병 발생이 완전히 방제되어, 품질이 우수한 콩나물을 생산하게 되었다 따라서 이는 확립된 부패방지기술로서 적용이 가능하다고 판단되었다.

Keywords

References

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  1. Determination of the variations in levels of phenolic compounds in soybean (Glycine maxMerr.) sprouts infected by anthracnose (Colletotrichum gloeosporioides) vol.93, pp.12, 2013, https://doi.org/10.1002/jsfa.6142