Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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DNA Markers Applicable for Identification of Two Internal Apple Feeders, Grapholita molesta and Carposina sasakii

두 종의 사과 심식나방류 [복숭아순나방 (Grapholita molesta), 복숭아심식나방 (Carposina sasakii)] 동정용 DNA 분자지표

  • Song, Seung-Baeck (Department of Bioresource Sciences, Andong National University) ;
  • Choi, Kyeung-Hee (Apple Experiment Station, National Horticultural Research Institute) ;
  • Lee, Soon-Won (Apple Experiment Station, National Horticultural Research Institute) ;
  • Kim, Yong-Gyun (Department of Bioresource Sciences, Andong National University)
  • 송승백 (안동대학교 생명자원과학과) ;
  • 최경희 (농촌진흥청 원예연구소 사과시험장) ;
  • 이순원 (농촌진흥청 원예연구소 사과시험장) ;
  • 김용균 (안동대학교 생명자원과학과)
  • Published : 2007.08.30
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Abstract

Two fruit moths of the oriental fruit moth, Grapholita molesta (Busck), and the peach fruit moth, Carposina sasakii (Matsumura), infest apples in Korea by internally feeding behavior. C. sasakii is a quarantine insect pest from some other countries importing Korean apples. G. molesta is not a quarantine insect pest, but can be incorrectly identified as C. sasakii especially when it is found inside apple fruits at its larval stages because it is not easy to identify the two species by morphological characters alone. This incomplete identification results in massive economical loss by fruits needlessly destroyed or turned away at border inspection stations of the importing nations. This difficulty can be overcome by molecular DNA markers. Several polymorphic regions of mitochondrial DNA of both species were sequenced and used for developing specific striction sites and polymerase chain reaction (PCR) primers. Based on these sequences, three diagnostic PCR-restriction fragment length polymorphism (RFLP) sites were detected and validated for their practical uses. Also, species-specific PCR primers were devised to develop diagnostic PCR method for identifying the internal feeders.

국내 서식하는 복숭아순나방 (Grapholita molesta (Busck))과 복숭아심식나방 (Carposina sasakii (Matsumura))의 유충은 사과 과실내부를 섭식하여 피해를 주는 해충이다. 사과를 수출할 때 복숭아심식나방은 수출대상국들로부터 검역 대상해충이다. 반면에 복숭아순나방은 광범위한 분포로 비교적 수입국으로부터 검역 대상 해충은 아니지만, 사과 과실 내부에서 발견되는 경우 복숭아심식나방으로 오인될 수 있다. 이는 발견되는 유충을 가지고 형태적으로 두 종을 구분하기 어렵기 때문이다. 특별히 수입국 검역단계에서 이러한 불완전한 동정 실태는 수출 사과의 폐기 또는 반송과 수출중단 등과 같은 막대한 경제적 손실을 초래하게 된다. 이에 이들을 구분할 수 있는 분자지표 개발이 요구되었다. 두 종의 미토콘드리아 DNA를 대상으로 다형을 보이는 여러 영역의 염기서열을 분석하였다. 이 서열을 바탕으로 진단용 제한위치가 결정되고 종 특이적 프라이머가 제작되었다. 본 연구는 세 부위의 종 특이적 제한효소 위치에 따라 PCR-RFLP 기술과 종 특이적 프라이머를 이용하여 진단용 PCR 기술을 개발하였다.

Keywords

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