Journal of Microbiology and Biotechnology

  • 제17권7호
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  • Pages.1113-1119
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  • 2007
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Effects of Culture Conditions on Osteogenic Differentiation in Human Mesenchymal Stem Cells

  • Song, Su-Jin (Department of Chemical Engineering, Hanyang University) ;
  • Jeon, O-Ju (Department of Chemical Engineering, Hanyang University) ;
  • Yang, Hee-Seok (Department of Bioengineering, Hanyang University) ;
  • Han, Dong-Keun (Biomaterials Research Center, Korea Institute of Science and Technology) ;
  • Kim, Byung-Soo (Department of Bioengineering, Hanyang University)
  • 발행 : 2007.07.31
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초록

Human bone marrow-derived mesenchymal stem cells (hBMMSCs) must differentiate into osteogenic cells to allow for successful bone regeneration. In this study, we investigated the effects of different combinations of three soluble osteogenic differentiation-inducing factors [L-ascorbic acid (AC), ${\beta}$-glycerophosphate (${\beta}G$), and bone morphogenic protein-2 (BMP-2)] and the presence of a hydroxyapatite (HA) substrate on hBMMSC osteogenic differentiation in vitro. hBMMSCs were cultured in medium containing various combinations of the soluble factors on culture plates with or without HA coating. After 7 days of culture, alkaline phosphatase (ALP) activity, calcium deposition, and osteoprotegerin (OPG) and osteopontin (OPN) expression were measured. The effects of individual and combined factors were evaluated using a factorial analysis method. BMP-2 predominantly affected expression of early markers of osteogenic differentiation (ALP and OPG). HA had the highest positive effect on OPN expression and calcium deposition. The interaction between AC, ${\beta}G$, and HA had the second highest positive effect on ALP activity.

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참고문헌

  1. Agrawal, M. K., S. K. Ghosh, D. Bagchi, J. Weckesser, M. Erhard, and S. N. Bagchi. 2006. Occurrence of microcyst incontaining toxic water blooms in central India. J. Microbiol. Biotechnol. 16: 212-218
  2. Anderson, H. C., J. B. Sipe, L. Hessle, R. Dhanyamrctiu, E. Atti, N. P. Camacho, and J. L. Millan. 2004. Impaired calcification around matrix vesicles of growth plate and bone in alkaline phosphatase-deficient mice. Am. J. Pathol. 164: 841-847 https://doi.org/10.1016/S0002-9440(10)63172-0
  3. Brugge, T. P. and J. A. Jansen. 2002. In vitro osteogenic differentiation of rat bone marrow cells subcultured with and without dexamethasone. Tissue Eng. 8: 321-331 https://doi.org/10.1089/107632702753725076
  4. Centrella, M., M. C. Horowitz, J. M. Wozney, and T. L. McCarthy. 1994. Transforming growth factor-beta gene family members and bone. Endocr. Rev. 15: 27-39
  5. Chen, Y. L., H. C. Chen, H. P. Lee, H. Y. Chan, and Y. C. Hu. 2006. Rational development of GAG-augmented chitosan membranes by fractional factorial design methodology. Biomaterials 27: 2222-2232 https://doi.org/10.1016/j.biomaterials.2005.10.029
  6. Cheng, S. L., J. W. Yang, L. Rifas, S. F. Zhang, and L. V. Avioli. 1994. Differentiation of human bone marrow osteogenic stromal cells in vitro: Induction of the osteoblast phenotype by dexamethasone. Endocrinology 134: 277-286 https://doi.org/10.1210/en.134.1.277
  7. Choong, P. F. M., T. J. Martin, and K. W. Ng. 1993. Effects of ascorbic acid, calcitriol, and retinotic acid on the differentiation ofpreosteoblasts. J. Orthop. Res. 11: 638-647 https://doi.org/10.1002/jor.1100110505
  8. Chou, Y. F., W. Huang, J. C. Dunn, T. A. Miller, and B. M. Wu. 2005. The effect of biomimetic apatite structure on osteoblast viability, proliferation, and gene expression. Biomaterials 26: 285-295 https://doi.org/10.1016/j.biomaterials.2004.02.030
  9. Ciapetti, G, L. Ambrosio, G Marietta, N. Baldini, and A. Giunti. 2006. Human bone marrow stromal cells: In vitro expansion and differentiation for bone engineering. Biomaterials 27: 6150-6160 https://doi.org/10.1016/j.biomaterials.2006.08.025
  10. Fedde, K. N., L. Blair, J. Silverstein, S. P. Coburn, L. M. Ryan, R. S. Weinstein, K. Waymire, S. Narisawa, J. L. Millan, G. R. MacGregor, and M. P. Whyte. 1999. Alkaline phosphatase knock-out mice recapitulate the metabolic and skeletal defects of infantile hypophosphatasia. J. Bone Miner. Res. 14: 2015-2026 https://doi.org/10.1359/jbmr.1999.14.12.2015
  11. Gomes, M. E., H. L. Holtorf, R. L. Reis, and A. G. Mikos. 2006. Influence of the porosity of starch-based fiber mesh scaffolds on the proliferation and osteogenic differentiation of bone marrow stromal cells cultured in a flow perfusion bioreactor. Tissue Eng. 12: 801-809 https://doi.org/10.1089/ten.2006.12.801
  12. Haynesworth, S. E., J. Goshinia, V. M. Goldberg, and A. I. Caplan. 1992. Characterization of cells with osteogenic potential from human marrow. Bone 13: 81-88 https://doi.org/10.1016/8756-3282(92)90364-3
  13. Hessle, L., K. A. Johnson, H. C. Anderson, S. Narisawa, A. Sali, J. W. Goding, R. Terkeltaub, and J. L. Millan. 2002. Tissue-nonspecific alkaline phosphatase and plasma cell membrane glycoprotein-1 are central antagonistic regulators of bone mineralization. Proc. Natl. Aead. Sci. USA 99: 9445-9449
  14. Hofinann, S., H. Hagenmuller, A. M. Koch, R. Muller, G. Vunjak-Novakovic, D. L. Kaplan, H. P. Merkle, and L. Meinel. 2007. Control of in vitro tissue-engineered bone-like structures using human mesenchymal stem cells and porous silk scaffolds. Biomaterials 28: 1152-1162 https://doi.org/10.1016/j.biomaterials.2006.10.019
  15. Ito, C., W. E. Evans, L. McNinch, E. Coustan-Smith, H. Mahmoud, C. H. Pui, and D. Campana. 1996. Comparative cytotoxicity of dexamethasone and prednisolone in childhood acute lymphoblastic leukemia. J. Clin. Oncol. 14: 2370-2376 https://doi.org/10.1200/JCO.1996.14.8.2370
  16. Jagodzinski, M., M. Drescher, J. Zeichen, S. Hankemeier, C. Krettek, U. Bosch, and M. van Griensven. 2004. Effects of cyclic longitudinal mechanical strain and dexamethasone on osteogenic differentiation of human bone marrow stromal cells. Eur. Cell. Mater. 7: 35-41 https://doi.org/10.22203/eCM.v007a04
  17. Jager, M., T. Feser, H. Denck, and R. Krauspe. 2005. Proliferation and osteogenic differentiation of mesenchymal stem cells cultured onto three different polymers in vitro. Ann. Biomed. Eng. 33: 1319-1332 https://doi.org/10.1007/s10439-005-5889-2
  18. Jaiswal, N., S. E. Haynesworth, A. I. Caplan, and S. P. Bruder. 1997. Osteogenic differentiation of purified, cultureexpanded human mesenchymal stem cells in vitro. J. Cell. Bioehem. 64: 295-312 https://doi.org/10.1002/(SICI)1097-4644(199702)64:2<295::AID-JCB12>3.0.CO;2-I
  19. Kasten, P., J. Vogel, R. Luginbuhl, P. Niemeyer, S. Weiss, S. Schneider, M. Kramer, A. Leo, and W. Richter. 2006. Influence of platelet-rich plasma on osteogenic differentiation of mesenchymal stem cells and ectopic bone formation in calcium phosphate ceramics. Cells Tissues Organs 183: 68-79 https://doi.org/10.1159/000095511
  20. Kim, M. K. and C. Niyibizi. 2001. Interaction of TGF-betal and rhBMP-2 on human bone marrow stromal cells cultured in collagen gel matrix. Yonsei Med. J. 42: 338-344 https://doi.org/10.3349/ymj.2001.42.3.338
  21. Kim, S. S., M. S. Park, O. Jeon, C. Y. Choi, and B. S. Kim. 2006. Poly(lactide-co-glycolide)/hydroxyapatite composite scaffolds for bone tissue engineering. Biomaterials 27: 1399-1409 https://doi.org/10.1016/j.biomaterials.2005.08.016
  22. Kim, S. S., M. S. Park, S. J. Gwak, C. Y. Choi, and B. S. Kim. 2006. Accelerated bonelike apatite growth on porous polymer/ceramic composite scaffolds in vitro. Tissue Eng. 12: 2997-3006 https://doi.org/10.1089/ten.2006.12.2997
  23. Kotobuki, N., K. Ioku, D. Kawagoe, H. Fujimori, S. Goto, and H. Ohgushi. 2005. Observation of osteogenic differentiation cascade of living mesenchymal stem cells on transparent hydroxyapatite ceramics. Biomaterials 26: 779-785 https://doi.org/10.1016/j.biomaterials.2004.03.020
  24. Lecanda, F., L. V. Avioli, and S. L. Cheng. 1997. Regulation of bone matrix protein expression and induction of differentiation of human osteoblasts and human bone marrow stromal cells by bone morphogenetic protein-2. J. Cell. Biochem. 67: 386-396 https://doi.org/10.1002/(SICI)1097-4644(19971201)67:3<386::AID-JCB10>3.0.CO;2-B
  25. Maniatopoulos, C., J. Sodek, and A. Melcher. 1988. Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats. Cell Tissue Res. 254: 317-330
  26. Mauney, J. R., S. Sjostorm, J. Blumberg, R. Horan, J. P. O'Leary, G. Vunjak-Novakovic, V. Volloch, and D. L. Kaplan. 2004. Mechanical stimulation promotes osteogenic differentiation of human bone marrow stromal cells on 3-D partially demineralized bone scaffolds in vitro. Calcif. Tissue Int. 74: 458-468 https://doi.org/10.1007/s00223-003-0104-7
  27. Mendes, S. C., I. Van Den Brink, J. D. De Bruijn, and C. A. Van Blitterswijk. 1998. In vivo bone formation by human bone marrow cells: Effect of osteogenic culture supplements and cell densities. J. Mater. Sci. Mater. Med. 9: 855-858 https://doi.org/10.1023/A:1008904413859
  28. Osyczka, A. M. and P. S. Leboy. 2005. Bone morphogenetic protein regulation of early osteoblast genes in human marrow stromal cells is mediated by extracellular signalregulated kinase and phosphatidylinositol 3-kinase signaling. Endocrinology 146: 3428-3437 https://doi.org/10.1210/en.2005-0303
  29. Park, J. H., S. R. Yu, J. S. Yoon, and K. H. Baek. 2005. Highlevel expression of recombinant human bone morphogenetic protein-4 in Chinese hamster ovary cells. J. Microbiol. Biotechnol. 15: 1397-1401
  30. Pereira, R. C., A. M. Delany, and E. Canalis. 2002. Effects of cortisol and bone morphogenetic protein-2 on stromal cell differentiation: Correlation with CCAAT-enhancer binding protein expression. Bone 30: 685-691 https://doi.org/10.1016/S8756-3282(02)00687-7
  31. Pittenger, M. F., A. M. Mackay, S. C. Beck, R. K. Jaiswal, R. Douglas, J. D. Mosca, M. A. Moorman, D. W. Simonetti, S. Craig, and D. R. Marshak. 1999. Multilineage potential of adult human mesenchymal stem cells. Science 284: 143-147 https://doi.org/10.1126/science.284.5411.143
  32. Poliard, A., D. Lamblin, P. J. Marie, M. H. Buc-Caron, and O. Kellermann. 1993. Commitment of the teratocarcinomaderived mesodermal clone C1 towards terminal osteogenic differentiation. J. Cell Sci. 106: 503-512
  33. Riddle, R. C., A. F. Taylor, D. C. Genetos, and H. J. Donahue. 2006. MAP kinase and calcium signaling mediate fluid flow-induced human mesenchymal stem cell proliferation. Am. J. Physiol. Cell Physiol. 290: 776-784 https://doi.org/10.1152/ajpcell.00082.2005
  34. Shukunami, C., K. Ishizeki, T. Atsumi, Y. Ohta, F. Suzuki, and Y. Hiraki. 1997. Cellular hypertrophy and calcification of embryonal carcinoma-derived chondrogenic cell line ATDC5 in vitro. J. Bone Miner. Res. 12: 1174-1188 https://doi.org/10.1359/jbmr.1997.12.8.1174
  35. Simmons, C. A., S. Matlis, A. J. Thornton, S. Chen, C. Y. Wang, and D. J. Mooney. 2003. Cyclic strain enhances matrix mineralization by adult human mesenchymal stem cells via the extracellular signal-regulated kinase (ERK 1/2) signaling pathway. J. Biomech. 36: 1087-1096 https://doi.org/10.1016/S0021-9290(03)00110-6
  36. Song, H. B., D. C. Park, G. M. Do, S. L. Hwang, W. K. Lee, H. S. Kang, B. R. Park, H. J. Jang, C. W. Son, E. K. Park, S. Y. Kim, and T. L. Huh. 2006. Effect of exopolymers of Aureobasidium pullulans on improving osteoporosis induced in ovariectomized mice. J. Microbiol. Biotechnol. 16: 37-45
  37. Yamaguchi, A., T. Ishizuya, and N. Kintou. 1996. Effects of BMP-2, BMP-4, and BMP-6 on osteoblastic differentiation of bone marrow-derived stromal cell lines, ST2 and MC3T3G2/PA6. Biochem. Biophys Res. Commun. 220: 366-371 https://doi.org/10.1006/bbrc.1996.0411
  38. Yamamoto, N., K. Furuya, and K. Hanada. 2002. Progressive development of the osteoblast phenotype during differentiation of osteoprogenitor cells derived from fetal rat calvaria: Model for in vitro bone formation. Biol. Pharm. Bull. 25: 509-515 https://doi.org/10.1248/bpb.25.509
  39. Yang, H. S., G D. Park, K. D. Ahn, B. S. Kim, and D. K. Han. 2006. Optimal hydrophilization and chondrocyte adhesion of PLLA and scaffolds by plasma treatment and acrylic acid grafting. Polymer (Korea) 30: 168-174
  40. Yoneno, K., S. Ohno, K. Tanimoto, K. Honda, N. Tanaka, T. Doi, T. Kawata, E. Tanaka, S. Kapila, and K. Tanne. 2005. Multidifferentiation potential of mesenchymal stem cells in three-dimensional collagen gel cultures. J. Biomed. Mater. Res. A 75: 733-741
  41. Zhang, R. and P. X. Ma. 2004. Biomimetic polymer/apatite composite scaffolds for mineralized tissue engineering. Macromol. Biosci. 4: 100-111 https://doi.org/10.1002/mabi.200300017
  42. Zhao, F., R. Chell a, and T. Ma. 2006. Effects of shear stress on 3-D human mesenchymal stem cell construct development in a perfusion bioreactor system: Experiments and hydrodynamic modeling. Biotechnol. Bioeng. 96: 584-595