Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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A Study on the Oogeneis of False Dace (Pseudorasbora parva)

참붕어 (Pseudorasbora parva)의 난자형성과정에 관한 연구

  • Kim, Dong-Heui (Department of Basic Science, Wonju College of Medicine, Yonsei University) ;
  • Lee, Kyu-Jae (Department of Parasitology, Wonju College of Medicine, Yonsei University) ;
  • Kim, Seok (Electron Microscope Facility Wonju College of Medicine, Yonsei University) ;
  • Deung, Young-Kun (Department of Basic Science, Wonju College of Medicine, Yonsei University)
  • 김동희 (연세대학교 원주의과대학 기초과학교실) ;
  • 이규재 (연세대학교 원주의과대학 기생충학교실) ;
  • 김석 (연세대학교 원주의과대학 전자현미경실) ;
  • 등영건 (연세대학교 원주의과대학 기초과학교실)
  • Published : 2007.06.30
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Abstract

The oogenesis and ultrastructure of fertilized egg envelope of false dace were investigated by light and electron microscope. The cytoplasm of false dace oogonia was basophilic and many nucleoli were located at inner side of nuclear membrane. In primary oocytes, yolk vesicles were distributed in marginal area only and egg envelope was not formed on egg outside. In secondary oocyte, the egg envelope was formed and yolk vesicles were increased than that of early stage in cytoplasm. The amount of basophilic substance was decreased. In case of matured egg, thickness of egg envelope and site of egg were increased, basophilic substance was distributed in egg envelope around only. The yolk vesicles were changed to yolk mass in accordance with development. The fertilized egg was of ellipsoidal, adhesive type and yellowish, have a single micropyle in the area of the animal pole. The fertilized egg envelope consisted of three layers, an outer adhesive layer, a middle layer consisting of 6 lamellae alternating layers and an inner electron dense layer. An outer surface of the fertilized egg envelope was arranged by adhesive fibrous structures. In conclusion, it is summarized that the oogenesis of false dace were the increase of cell size, the formation and accumulation of yolk, and decrease of basophilic intensity in cytoplasm. These ultrastructural characteristics of fertilized egg envelope from false dace can be utilized in taxonomy of teleost.

참붕어의 난자형성과정과 수정란 난막의 미세구조를 광학현미경과 전자현미경으로 관찰한 결과는 다음과 같다. 참붕어에서 난원세포의 세포질은 호염기성이었고 핵막 내에 많은 인들이 분포하고 있었다. 제1난모세포의 경우 난황포가 단지 난세포 가장자리에만 배열되어 있었고 난막의 형성은 관찰되지 않았다. 제2난모세포에서는 난막이 형성되었고 제1난모세포에 비해서 난황포가 점점 핵쪽으로 증식된 경향을 보였다. 발생이 진행됨에 따라서 호염기성 물질들은 점점 감소하는 경향을 보였고 후에 난막주위에만 국한적으로 분포하였으며, 난막의 두께와 난자의 크기는 점점 증가되었다. 또한 성숙란으로 발생이 진행되면서 난황포들은 난황괴로 변화되었다. 참붕어의 수정란은 타원형으로 부착성이었으며 동물극쪽에서 난문이 관찰되었다. 수정란의 난막은 모두 3층으로, 부착성인 외층, 전자밀도가 서로 다른 6층상구조를 가진 중층 및 전자밀도가 높은 내층으로 구성되어 있었으며 난막표면은 부착성 섬유상 구조물로 덮여 있었다. 이상과 같이 참붕어의 난자형성과정은 생식세포의 크기 증가, 난황의 형성과 축적 및 세포질의 호염기성물질 감소로 될 수 있으며 수정란 난막의 미세구조적 특징들은 경골어류의 계통분류학에서 분류형질도 이용될 수 있을 것으로 생각된다.

Keywords

References

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