Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Protective Antibodies and Immunity elicited by Immunization with Outer Membrane Protein H of Pasteurella multocida in Mice

Pasteurella multocida의 외막 단백질 H에 의해 유도되는 방어적 항체와 면역

  • Kwon, Moo-Sik (Department of Genetic Engineering, Sungkyunkwan University) ;
  • Kim, Young-Bong (Department of Animal Biotechnology, Konkuk University) ;
  • Lee, Jeong-Min (Department of Animal Biotechnology, Konkuk University)
  • 권무식 (성균관대학교 유전공학과) ;
  • 김영봉 (건국대학교 동물생명공학과) ;
  • 이정민 (건국대학교 동물생명공학과)
  • Published : 2007.03.31
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Abstract

Pasteurella multocida is one of the important animal pathogen causing widespread infections in various domestic animals. In swine, it causes severe respiratory diseases such as atrophic rhinitis and pneumonic pasteurellosis. To develop the efficient subunit vaccine against swine atrophic rhinitis, we investigated protective antibodies and humoral immunity of outer membrane protein H (OmpH) which is one of the major outer membrane proteins in P. multocida. Outer membrane fraction of P. multocida was immunologically detectable using antisera from both mice groups vaccinated by formalin-killed whole cells and by commercial vaccine. The expression vector for production of recombinant OmpH was constructed and the recombinant OmpH was expressed and purified from E. coli. Recombinant OmpH showed high antigenic and immunogenic properties in mice vaccination and ELISA with antisera.

Pasteurella multocida는 돼지에서 위축성 비염, 폐렴을 비롯한 다양한 호흡기 질환을 일으키는 병원균이다. 본 연구에서는 돼지 위축성 비염에 대한 효과적인 순수정제 백신을 개발하고자 하는 기초 연구로서 P. multocida의 외막 단백질 H에 의해 유도되는 방어적 항체와 면역을 확인하였다. P. multocida의 외막 단백질을 포함하는 분획은 호흡기 질병 혼합 백신에 대한 항혈청과 불활화된 사균 세포에 대한 항혈청 모두에서 면역학적으로 검출 가능하였다. 선행 연구에서 분리한 외막 단백질 H 유전자는 재조합 발현 백터 제작에 이용되어 대장균으로부터 재조합 외막단백질 H를 정제하였다. 실험 동물 면역과 항혈청의 교차반응, ELISA를 통한 항체 역가의 측정 및 공격접종을 통하여, 재조합 외막 단백질 H는 높은항원성을 가지며, 지속적인 체액성 면역을 유도하는 것을 확인하였다. 외막 단백질 H는 순수정제 항원으로서 P. multocida에 의한 호흡기 질환에 대한 효과적인 방어를 유도할 수 있는 단위 백신 후보 단백질로 여겨진다.

Keywords

References

  1. 김봉환, 탁연빈, 조길재, 장희경. 1991. 돼지 전염성 위축성 비염의 임상학적 및 세균학적 연구. 대한수의학회지 31, 457-469
  2. 지영철, 로승, 한정희, 한태욱. 2000. 돼지 위축성 비염백신의 효과에 관한 연구. 대한수의학회지 40, 707-717
  3. Adler, B., D. Bulach, J. Chung, S. Doughty, M. Hunt, K Rajakumar, M. Serrano, A van Zanden, Y. Zhang, and C. Ruffolo. 1991. Candidate vaccine antigens and genes in Pasteurella multocida. J. Biotechnol. 73, 83-90 https://doi.org/10.1016/S0168-1656(99)00111-X
  4. Carter, G.R. 1967. Pasteurellosis: Pasteurella multocida and Pasteurella heinolytica. Adv. Vet. Sci. Camp. Med. 11, 321-379
  5. Christodoulides, M., B.T. McGuinness, and J.E. Heckels. 1993. Immunization with synthetic peptides containing epitopes of the class 1 outer-membrane protein of Neisseria meningitidis: production of bactericidal antibodies on immunization with a cyclic peptide. J. Gen. Microbiol. 139, 1729-1738 https://doi.org/10.1099/00221287-139-8-1729
  6. Gatto, N.T., S.M. Dabo, R.E. Hancock, and A.W. Confer. 2002. Characterization of, and immune responses of mice to, the purified OmpA-equivalent outer membrane protein of Pasteurella multacida serotype A:3 (Omp28). Vet. Microbiol. 87, 221-235 https://doi.org/10.1016/S0378-1135(02)00068-8
  7. Harper, M., J. D. Boyce, and B. Adler. 2006. Pasteurella multacida pathogenesis: 125 years after Pasteur. FEMS Microbiol. Lett. 265, 1-10 https://doi.org/10.1111/j.1574-6968.2006.00442.x
  8. Heddleston, K.H., J.E. Gallagher, and P.A. Rebers. 1972. Fowl cholera: gel diffusion precipitin test for serotyping Pasteurella multocida from avian species. Avian Dis. 16, 925-936 https://doi.org/10.2307/1588773
  9. Hunt, M.L., B. Adler, and K.M. Townsend. 2000. The molecular biology of Pasteurella multocida. Vet. Microbiol. 72, 3-25 https://doi.org/10.1016/S0378-1135(99)00183-2
  10. Lee, J., S. Kang, S.I. Park, H.J. Woo, and M. Kwon. 2004. Molecular cloning and characterization of the gene for outer memebrane protein H in a Pasteurella multocida (D:4) isolate from pigs with atrophic rhinitis symptoms in Korea. J. Microbiol. Biotechnol. 14, 1343-1349
  11. Liao, C.M., C. Huangb, S.L. Hsuan, Z.W. Chenb, W.C. Lee, C.I. Liu, J.R Winton, and M.S. Chien. 2006.Immunogenicity and efficacy of three recombinant subunit Pasteurella multocida toxin vaccines against progressive atrophic rhinitis in pigs. Vaccine 24, 27-35 https://doi.org/10.1016/j.vaccine.2005.07.079
  12. Luo, Y., J.R. Glisson, M.W. Jackwood, R.E. Hancock, M. Bains, I.H. Cheng, and C. Wang. 1997. Cloning and characterization of the major outer membrane protein gene (ompH) of Pasteurella multocida X-73. J. Bacteriol. 179, 7856-7864 https://doi.org/10.1128/jb.179.24.7856-7864.1997
  13. Luo, Y., Q. Zeng, J.R. Glisson, M.W. Jackwood, I.H. Cheng, and C. Wang. 1999. Sequence analysis of Pasteurella multocidamajor outer membrane protein (OmpH) and application of synthetic peptides in vaccination of chickens against homologous strain challenge. Vaccine 17, 821-831 https://doi.org/10.1016/S0264-410X(98)00266-7
  14. Petersen, S.K., N.T. Foged, A. Bording, J.P. Nielsen, H.K. Riemann, and P.L. Frandsen. 1991. Recombinant derivatives of Pasteurella multocida toxin: candidates for a vaccine against progressive atrophic rhinitis. Infect. Immun. 59, 1387-1393
  15. Poolman, J.T., L. Bakaletz, A. Cripps, P.A. Denoel, A. Forsgren, J. Kyd, and Y. Lobet. 2000. Developing a nontypeable Haemophilus influenzae (NTHi) vaccine. Vaccine 19 (suppl 1), S108 https://doi.org/10.1016/S0264-410X(00)00288-7
  16. Rajeev, S., R.V. Nair, S.A. Kania, and D.A. Bemis. 2003. Expression of a truncated Pasteurella multocida toxin antigen in Bordetella bronchiseptica. Vet. Microbiol. 94, 313-323 https://doi.org/10.1016/S0378-1135(03)00137-8
  17. Rhoades, K.R. and R. B. RimIer. 1987. Capsular groups of Pasteurella multocidaisolated from avian hosts. Avian Dis. 31, 895-898 https://doi.org/10.2307/1591048
  18. Rutter, J.M. and X. Rojas. 1982. Atrohpic rhinitis in gnobiotic piglets: differences in the pathogenicity of Pasteurella multocida in combined infections with Bordetella bronchiseptica. Vet. Res. 110, 531-535
  19. Simons, K.R., R.J. Morton, R.W. Fulton, and A.W. Confer. 1992. Comparison of antibody responses in cattle to outer membrane proteins from Pasteurella haemolytica serotype I and from eight untypeable strains. Am. J. Ves. Res. 53, 971-975
  20. To, H., S. Someno, and S. Nagai. 2005. Development of a genetically modified nontoxigcnic Pasteurella multocida toxin as a candidate for use in vaccines against progressive atrophic rhinitis in pigs. Am. J. Vet. Res. 66, 113-120 https://doi.org/10.2460/ajvr.2005.66.113
  21. Vasfi, M. and K.R. Mittal. 1997. Role of outer membrane protein H (OmpH)- and OmpA-specific monoclonal antibodies from hybridoma tumors in protection of mice against Pasteurella multocida. Infect. Immun. 65, 4502-4508