Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Revegetation of a Lakeside Barren Area by the Application of Plant Growth-promoting Rhizobacteria

  • Ahn, Tae-Seok (Department of Environmental Science, Kangwon National University) ;
  • Ka, Jong-Ok (School of Agricultural Biotechnology, Seoul National University) ;
  • Lee, Geon-Hyoung (Department of Biological Science, Kunsan National University) ;
  • Song, Hong-Gyu (Division of Life Sciences, Kangwon National University)
  • Published : 2007.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

The growth stimulation of wild plants by several bacterial species showing plant growth-promoting capabilities was examined in a barren lakeside area at Lake Paro, Korea. Microbial numbers and activities in the field soil were monitored for 73 days after inoculation of the bacteria. The acridine orange direct counts for the total soil bacterial populations ranged between $2.0-2.3{\times}10^{9}\;cells/g$ soil and $1.4-1.8{\times}10^{9}\;cells/g$ soil in the inoculated and uninoculated soils, respectively. The numbers of Pseudomonas spp., which is known as a typical plant growth-promoting rhizobacteria, and the total microbial activity were higher in the inoculated soil compared to those in the uninoculated soil. The average shoot and root lengths of the wild plants grown in the inoculated soil were 17.3 cm and 12.4 cm, respectively, and longer than those of 11.4 cm and 8.5 cm in the uninoculated soil. The total dry weight of the harvested wild plants was also higher in the inoculated soil (42.0 g) compared to the uninoculated soil (35.1 g). The plant growth-promoting capabilities of the inoculated bacteria may be used for the rapid revegetation of barren or disturbed land, and as biofertilizer in agriculture.

Keywords

References

  1. Ahn, T., H. Song, and J. Park. 2004. Rehabilitation of naked shoreline of lakes and reservoirs. Report No. 1403013-1-1. Korea Institute of Environmental Science and Technolog
  2. Bashan, Y. and L.E. de-Bashan. 2005. Fresh-weight measurements of roots provide inaccurate estimates of the effects of plant growth-promoting bacteria on root growth: a critical examination. Soil Biol. Biochem. 37, 1795-1804 https://doi.org/10.1016/j.soilbio.2005.02.013
  3. Bertland, H., R. Nalin, R. Bally, and J.C. Cleyet-Marel. 2001. Isolation and identification of the most efficient plant growth promoting bacteria associated with canola (Brassica napus). Biol. Fertil. Soils 33, 152-156 https://doi.org/10.1007/s003740000305
  4. Cakmakci, R., F. Domez, A. Aydin, and F. Sahin. 2006. Growth promotion of plants by plant growth-promoting rhizobacteria under greenhouse and two different field soil conditions. Soil Biol. Biochem. 38, 1482-1487 https://doi.org/10.1016/j.soilbio.2005.09.019
  5. Carrilo-Garcia, A., Y. Bashan, E. Rivera, and G. Bethlenfalvay. 2000. Effects of resource-island soils, competition, and inoculation with Azospirillum on survival and growth of Pachycereus pringlei, the giant cactus of the Sonoran desert. 8, 65-73 https://doi.org/10.1046/j.1526-100x.2000.80009.x
  6. Chanway, C.P. 1997. Inoculation of tree roots with plant growth promoting soil bacteria: An emerging technology for reforestation. For. Sci. 43, 99-112
  7. Gamalero, E., L. Fracchia, M. Cavaletto, J. Garbaye, P. Frey-Klett, G. Varese, and M. Martinotti. 2003. Characterization of functional traits of two fluorescent pseudomonads isolated from basidiomes of ectomycorrhizal fungi. Soil Biol. Biochem. 35, 55-65 https://doi.org/10.1016/S0038-0717(02)00236-5
  8. Gerhardson, B. and S. Wright. 2002. Bacterial associations with plants: Beneficial, non N-fixing interactions, p. 79-103, In K. Sivasithamparam, K. Dixon, and R. Narrett (eds.), Microorganism in plant conservation and biodiversity. Kluwer Academic Press, London, UK
  9. Hobbie, J., R. Daley, and S. Japer. 1977. Use of Nuclepore filters for counting bacteria by fluorescence microscopy. Appl. Environ. Microbiol. 33, 1225-1228
  10. Jeon, J.S., S.S. Lee, H.Y. Kim, T.S. Ahn, and H.G. Song. 2003. Plant growth promotion in soil by some inoculated microorganisms. J. Microbiol. 41, 271-276
  11. Kloepper, J., R. Lifshits, and R. Zablotowicz. 1989. Free living bacterial inocula for enhancing crop productivity. Trends Biotechnol. 7, 39-44 https://doi.org/10.1016/0167-7799(89)90057-7
  12. Kokalis-Burelle, N., J. Kloepper, and M. Reddy. 2006. Plant growthpromoting rhizobacteria as transplant amendments and their effects on indigenous rhizosphere microorganisms. Appl. Soil Ecol. 31, 91-100 https://doi.org/10.1016/j.apsoil.2005.03.007
  13. Lucas-Garcia, J., J. Domenech, C. Santamaria, M. Camacho, A. Daza, and F. Gutierrez-Manero. 2004. Growth of forest plants (pine and holm-oak) inoculated with rhizobacteria: relationship with microbial community structure and biological activity of its rhizosphere. Environ. Exp. Botany 52, 239-251 https://doi.org/10.1016/j.envexpbot.2004.02.003
  14. Lucy, M., E. Reed, and B. Glick. 2004 Application of free living plant growth-promoting rhizobacteria. Antonie van Leeuwenhoek 86, 1-25 https://doi.org/10.1023/B:ANTO.0000024903.10757.6e
  15. Misko, A. and J. Germida. 2002. Taxonomic and functional diversity of pseudomonads isolated from the roots of field-grown canola. FEMS Microbiol. Ecol. 42, 399-407 https://doi.org/10.1111/j.1574-6941.2002.tb01029.x
  16. Prosser, J.I. 1997. Microbial processes within the soil, p. 183-213. In J. van Elsas, J. Trevors, and E. Wellington (eds.), Modern Soil Microbiology. Marcel Dekker, Inc., New York, USA
  17. Shishido, M. and C. Chanway. 1998. Forest soil community responses to plant growth-promoting rhizobacteria and spruce seedlings. Biol. Fertil. Soils 26, 179-186 https://doi.org/10.1007/s003740050365
  18. Strigul, N. and L. Kravchenko. 2006. Mathematical modeling of PGPR inoculation into the rhizosphere. Environ. Model Softw. 21, 1158-1171 https://doi.org/10.1016/j.envsoft.2005.06.003
  19. Vessey, J.K. 2003. Plant growth promoting rhizobacteria as biofertilizers. Plant Soil 255, 571-586 https://doi.org/10.1023/A:1026037216893
  20. Yeom, J.R. and C.S. Park. 1995. Enhancement of plant growth and suppression of damping-off of cucumber by low temperature growing Pseudomonas fluorescens isolates. Kor. J. Plant Pathol. 11, 252-257