Journal of Microbiology

  • 제45권2호
  • /
  • Pages.168-170
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  • 2007
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  • 1225-8873(pISSN)
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  • 1976-3794(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Mutations in the GyrA Subunit of DNA Gyrase and the ParC Subunit of Topoisomerase IV in Clinical Strains of Fluoroquinolone-Resistant Shigella in Anhui, China

  • Hu, Li-Fen (Department of Infectious Diseases, the First Affiliated Hospital of Anhui Medical University) ;
  • Li, Jia-Bin (Department of Infectious Diseases, the First Affiliated Hospital of Anhui Medical University) ;
  • Ye, Ying (Department of Infectious Diseases, the First Affiliated Hospital of Anhui Medical University) ;
  • Li, Xu (Department of Infectious Diseases, the First Affiliated Hospital of Anhui Medical University)
  • 발행 : 2007.04.30
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초록

In this research 26 Shigella isolates were examined by PCR and direct nucleotide sequencing for genetic alterations in the quinolone-resistance determining regions (QRDRs). We tested for the presence of qnr genes by PCR in 91 strains, but no qnr genes were found. The results did show, however, some novel mutations at codon 83 of gyrA ($Ser{\rightarrow}Ile$) and codon 64 of parC ($Ala64{\rightarrow}Cys,\;Ala64{\rightarrow}Asp$), which were related to fluroquinolone resistance.

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참고문헌

  1. Ahamed, J., J. Gangopadhyay, M. Kundu, and A.K. Sinha. 1999. Mechanisms of quinolone resistance in clinical isolates of Shigella dysenteriae. Antimicrob. Agents Chemother. 43, 2333-2334
  2. Bhattacharya, S.K., K. Sarkar, B.G. Nair, A.S. Faruque, and D.A. Sack. 2003. Multidrug-resistant Shigella dysenteriae type 1 in south Asia. Lancet. Infect. Dis. 3, 755 https://doi.org/10.1016/S1473-3099(03)00829-6
  3. Clinical and Laboratory Standards Institute (CLSI). 2005. Performance standards for antimicrobial susceptibility testing, Fifteenth informational supplement M100-S15. 14th Ed. CLSI, Wayne, PA, USA
  4. Dutta, S., Y. Kawamura, T. Ezaki, G.B. Nair, K-I.I. Iida, and S-I. Yoshida. 2005. Alteration in the GyrA subunit of DNA gyrase and the ParC subunit of topoisomerase IV in quinoloneresistant Shigella dysenteriae serotype 1 clinical isolates from Kolkata, India. Antimicrob. Agents Chemother. 49, 1660-1661 https://doi.org/10.1128/AAC.49.4.1660-1661.2005
  5. Hiasa, H. 2002. The Glu-84 of the ParC subunit plays critical roles in both topoisomerase IV-quinolone and topoisomerase IV-DNA interactions. Biochemistry 41, 11779-11785 https://doi.org/10.1021/bi026352v
  6. Hirose, K., J. Terajima, H. Izumiya, K. Tamura, E. Arakawa, N. Takai, and H. Watanabe. 2005. Antimicrobial susceptibility of Shigella sonnei isolates in Japan and molecular analysis of S. sonnei isolates with reduced susceptibility to fluoroquinolones. Antimicrob. Agents Chemother. 49, 1203-1205 https://doi.org/10.1128/AAC.49.3.1203-1205.2005
  7. Mark, C.S., H. Chad, C. Christina, J. Nilofer, M. Nicholas, G. Jonathan, D. Beth, J.S. Karen, H.M. George, H. Roberta, and S. George. 2001. Antibiotic susceptibility profiles of Escherichia coli trains lacking multidrug efflux pump genes. Antimicrob. Agents Chemother. 45, 1126-1136 https://doi.org/10.1128/AAC.45.4.1126-1136.2001
  8. Naheed, A., P. Kalluri, K.A. Talukder, A.S. Faruque, F. Khatun, G.B. Nair, E.D. Mintz, and R.F. Breiman. 2004. Fluoroquinolone- resistant Shigella dysenteriae type 1 in northeastern Bangladesh. Lancet. Infect. Dis. 4, 607-608 https://doi.org/10.1016/S1473-3099(04)01143-0
  9. Robicsek, A., J. Strahilevitz, D.F. Sahm, G.A. Jacoby, and D.C. Hooper. 2006a. qnr pevalence in ceftazidime-resistant Enterobacteriaceae isolates from the United States. Antimicrob. Agents Chemother. 50, 2872-2874 https://doi.org/10.1128/AAC.01647-05
  10. Robicsek, A., J. Strahilevitz, G.A. Jacoby, M. Mark, A. Darren, P.H. Chi, B. Carren, and D.C. Hooper. 2006b. Fluoroquinolone modifying enzyme: a new adaptation of a common aminoglycoside acetyltransferase. Nat. Med. 12, 83-88 https://doi.org/10.1038/nm1347