Journal of the Korean Wood Science and Technology

The Korean Society of Wood Science & Technology (한국목재공학회)
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Biobleaching of Softwood Kraft Pulp Using Recombinant Xylanase and Cellulase

재조합 Xylanase와 Cellulase의 연속처리에 의한 침엽수 Kraft 펄프의 Biobleaching

  • Kim, Hyun Joo (BK21 : Added-value utilization of lignocellulosic biomass, College of Agriculture & Life Science, Chonnam National University) ;
  • Wi, Seung Gon (BK21 : Added-value utilization of lignocellulosic biomass, College of Agriculture & Life Science, Chonnam National University) ;
  • Bae, Hyeun-Jong (BK21 : Added-value utilization of lignocellulosic biomass, College of Agriculture & Life Science, Chonnam National University)
  • 김현주 (전남대학교 농업생명과학대학 BK21 목질 자원의 고도 이용팀) ;
  • 위승곤 (전남대학교 농업생명과학대학 BK21 목질 자원의 고도 이용팀) ;
  • 배현종 (전남대학교 농업생명과학대학 BK21 목질 자원의 고도 이용팀)
  • Received : 2007.10.20
  • Accepted : 2007.10.30
  • Published : 2007.11.25
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Abstract

To know the effect of enzymatic pre-treatment on softwood Kraft pulp, two xylanse-encoding genes, named xynl and xynll were isolated from Thrichoderma ressei. Structural genes of xylanase (XYNI, XYNII) and cellulase (EGIV-CBDII) were isolated from T. ressei and Rumicoccus albus respectively, and expressed in E. coli. bacterial culture. The specific activity of purified recombinant XYNI is higher than XYNII. The brightness of XYNI treated softwood Kraft pulp increased to 29.9%. On further sequential treatment with EGIV-CBDII and XYNI the brightness of softwood Kraft pulp were improved to 9.1 and 73% respectively. As expected the Kappa number of softwood Kraft pulp also decreased 8.1, 4.6 and 3.2% respectively. Results further indicate that this sequential combination of enzyme treatment has synergic effect for improving bleaching of softwood Kraft pulp.

효소 전처리에 의한 침엽수 Kraft 펄프의 표백 효율을 살펴보고자 Trichoderma reesei에서 2종류의 xylanase를 cloning하였다. Cloning된 xylanasel과 xylanaseII 유전자를 E. coli에서 발현시켜 29kDa의 재조합 단백질인 XYNI과 29.5 kDa의 XYNII를 생한하여 정제하였고, Rumicoccus albus로부터 cloning되어 변형된 50kDa의 EGIV-CBDII를 동일한 방법으로 cellulase를 생산 정제하였다. 정제된 효소들을 각각 xylan과 CMC를 기질로하여 효소 활성도를 측정한 결과 XYNI이 XYNII보다 효소활성이 더 높았으며, EFIV-CBDII 또한 높은 활성을 나타내었다. 이렇게 생산 정제된 xylanase와 cellulase를 이용하여 침엽수 Kraft 펄프를 대상으로 연속처리에 의한 biobleaching test를 실시하였다. XYNI을 처리하였을 때 백색도는 29.9% 증가하였고, XYNI를 1차 처리한 후 2차로 EGIV-CBDII를 처리하였을 때 백색도는 1차 처리구에 비해 9.1% 증가하였다. 그 후 3차 XYNI를 처리 하였을 때 2차에 비해 7.3%의 bleaching의 효과를 볼 수 있었다. 또한 Kappa No.는 처리단계에 따라 8.1, 4.6 그리고 3.2% 각각 지속적으로 감소하였다. 이 실험결과를 통해 재조합 효소 XYNI과 EGIV-CBDII를 함께 이용하여 Karft 펄프에 대한 biobleaching의 상승효과를 확인하였다.

Keywords

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