Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Teratogenicity Study of tert-Butyl Acetate in Rats

랫드에서 초산 제3부틸의 최기형성 시험

  • Ahn, Tai-Hwan (College of Veterinary Medicine, Chonnam National University) ;
  • Yang, Young-Su (College of Veterinary Medicine, Chonnam National University) ;
  • Lee, Jong-Chan (College of Veterinary Medicine, Chonnam National University) ;
  • Kang, Seong-Soo (College of Veterinary Medicine, Chonnam National University) ;
  • Bae, Chun-Sik (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Sung-Ho (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Jong-Choon (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Hyeon-Yeong (Chemical Safety & Health Research Center, Occupational Safety & Health Research Institute) ;
  • Chung, Yong-Hyun (Chemical Safety & Health Research Center, Occupational Safety & Health Research Institute)
  • 안태환 (전남대학교 수의과대학) ;
  • 양영수 (전남대학교 수의과대학) ;
  • 이종찬 (전남대학교 수의과대학) ;
  • 강성수 (전남대학교 수의과대학) ;
  • 배춘식 (전남대학교 수의과대학) ;
  • 김성호 (전남대학교 수의과대학) ;
  • 김종춘 (전남대학교 수의과대학) ;
  • 김현영 (산업안전보건연구원 산업화학물질연구센터) ;
  • 정용현 (산업안전보건연구원 산업화학물질연구센터)
  • Published : 2007.06.30
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Abstract

tert-Butyl acetate is an organic solvent used for coatings, industrial cleaning, and surface treatment applications. This study investigated the potential adverse effects of tert-butyl acetate on pregnant dams and embryo-fetal development after maternal exposure on gestational days 6 through 19 in rats. The test chemical was administered to pregnant rats by gavage at dose levels of 0, 500, 1,000, 1,500, and 2,000 mg/kg/day. All dams were subjected to a caesarean section on day 20 of gestation and their fetuses were examined for any external, visceral, and skeletal abnormalities. At 2,000 mg/kg, treatment-related clinical signs, including piloerection, abnormal gait, decreased locomotor activity, loss of fur, reddish tear, anorexia, nasal discharge, vocalization and coma, were observed in a dose-dependent manner. All dams died between the 2nd day and 5th day of treatment due to a severe systemic toxicity. At 1,500 mg/kg, minimal maternal toxicity including an increase in the incidence of decreased locomotor activity and loss of fur, and an increase in the weights of adrenal glands and liver was observed. On the contrary, no significant adverse effect on the embryo-fetal development was detected. There were no adverse effects on either pregnant dams or embryo-fetal development at <1,000 mg/kg. These results show that a 14-day repeated oral dose of tert-butyl acetate in rats caused a minimal maternal toxicity including increases in the incidence of clinical signs and the weights of adrenal glands and liver, but no embryotoxicity and teratogenicity at 1,500 mg/kg/day. Under these experimental conditions, the no-observed-adverse-effect level (NOAEL) of tert-butyl acetate is estimated to be 1,000 mg/kg per day for dams and 1,500 mg/kg per day for embryo-fetal development.

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References

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