Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Effects of Chitosan on Wound Healing in Pigs

돼지에서 창상치유에 대한 키토산의 영향

  • Byun, Hong-Sub (College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Myung-Jin (College of Veterinary Medicine, Chungnam National University) ;
  • Lee, Jae-Yeon (College of Veterinary Medicine, Chungnam National University) ;
  • Cho, Sung-Whan (College of Veterinary Medicine, Chungnam National University) ;
  • Park, Chang-Sik (Division of Animal Science & Resources, Research Center for Transgenic Cloned Pigs Chungnam National University) ;
  • Kim, Myung-Cheol (College of Veterinary Medicine, Chungnam National University)
  • 변홍섭 (충남대학교 수의과대학) ;
  • 김명진 (충남대학교 수의과대학) ;
  • 이재연 (충남대학교 수의과대학) ;
  • 조성환 (충남대학교 수의과대학) ;
  • 박창식 (충남대학교 동물자원학부, 형질전환복제돼지연구센터) ;
  • 김명철 (충남대학교 수의과대학)
  • Published : 2007.06.30
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Abstract

The objective of this study was to investigate the effects of implanted chitosan applied to surgically created wound in pigs. Six healthy $2{\sim}3$ months old Landrace and Yorkshire mixed breeds of both genders were used. A 2 cm straight skin incision was made and undermined skin ($2{\times}2cm$) over on the each pig's both sides of dorsal midline at 0, 7. 14 and 18 days. One wound (left side) was implanted 0.4 mg of cotton type chitosan and other wound was treated saline (3 ml). Each wound was closed with two interrupted suture of 2-0 sutures. The wounds created at 18, 14.7 and 0 days were named post-wounding day (PWD) 3, 7, 14 and 21, respectively. At 21 days after initial wounding, each wound was taken for histological observations. Reepithelialization tended to be greater in the chitosan group than in the control group at PWD 3 and 14. Granulation tissue formation did not show especial differences in two groups. Number of inflammatory cells was lesser statistically in level in the chitosan group than those in the control group at 21 days after wounding (p<0.05). Fibroblasts and neovasculature tended to be greater in the chitosan group than in the control group at PWD 3 and 7, and tended to be lesser in the chitosan group than in the control group at PWD 14 and 21. Collagen and fibrin were observed to be evenly distributed around the wound in the chitosan group. But collagen and fibrin were observed to be converged along the wound in the control group.

Keywords

References

  1. Hedlund GS. Wound management. In: Small animal surgery, 2nd ed. St Louis: Mosby. 2002: 134-144
  2. Hosgood G Wound repair and specific tissue response to injury. In: Textbook of small animal surgery, 3rd ed. Philadelphia: Saunders. 2003: 66-86
  3. Ishihara M, Ono K, Sato M, Nakanishi K, Saito Y, Yura H, Matsui T, Hattori H, Fujita M, Kikuchi M, Kurita A. Acceleration of wound contraction and healing with a photocrosslinkable chitosan hydrogel. Wound Rep Reg 2001; 9: 513-52 https://doi.org/10.1046/j.1524-475x.2001.00513.x
  4. Kim SK, Nghiep ND, Rajapakse N. Therapeutic prospectives of chitin, chitosan and their derivatives. J Chitin Chitosan 2006; 11: 1-10
  5. Kojima K, Okamoto Y, Kojima K, Miyatake K, Fujise H, Shigemasa Y, Minami S. Effects of chitin and chitosan on collagen synthesis in wound healing. J Vet Med Sci 66: 15951598, 2004 https://doi.org/10.1292/jvms.66.1595
  6. Mariappan MR, Alas EA, Williams JG, Prager MD. Chitosan and chitosan sulfate have opposing effects on collagenfibroblast interactions. Wound Rep Reg 1999; 7: 400-406
  7. Minami S, Oh-oka M, Okamoto Y, Miyatake K, Matsuhashi A, Shigemasa Y, Fukumoto Y. Chitosan-inducing hemorrhagic pnumonia in dogs. Carbohydrate Polymers 29: 241-246, 1996 https://doi.org/10.1016/0144-8617(95)00157-3
  8. Minami S, Okamoto Y, Matsuhashi A, Sashiwa H, Saimoto H, Shigemasa Y, Tanigawa T, Suzuki T, Tanioka S, Tanaka Y. Polymeric N-acetyl-D-giucosamine (chitin) induces prostaglandin $E_2$ in dogs. J Vet Med Sci 57: 377-378, 1995 https://doi.org/10.1292/jvms.57.377
  9. Minami S, Okamoto Y, Miyatake K, Matsuhashi A, Kitamulra Y, Tanigawa T, Tanaka Y, Shigemasa Y. Chitin induces type collagen and elastic fiber in implanted non-woven fabric of polyester. Carbohydrate Polymers 29: 295-299 https://doi.org/10.1016/S0144-8617(96)00078-1
  10. Nam KS, Cho HJ, Shon YH. Effect of chitosan oligosaccharide on chemopreventive en캉mes of colon cancer. J Chitin Chitosan 2006; 11: 171-174
  11. Okamoto Y, Minami S, Matsuhashi A, Sashiwa H, Saimoto H, Shigemasa Y, Tanigawa T, Tanaka Y, Tokura S. Application od polymeric N-acetyl-D-glucosamine (chitin) to Veterinary Practice. J Vet Med Sci 55: 743-747, 1993 https://doi.org/10.1292/jvms.55.743
  12. Okamoto Y, Shibazaki K, Minami S, Matsuhashi A, Tanioka S, Shigemasa Y. Evaluation of chitin and chitosan on open wound healing in dogs. J Vet Med Sci 57(5): 851-854, 1995 https://doi.org/10.1292/jvms.57.851
  13. Okamoto Y, Southwood L, Stashak TS, Norrdin RW, Nelson AW, Minami S, Matsuhashi A, Kato K, Shigemasa Y. Effect of chitin on nonwoven fabric implant in tendon healing Carbohydrate Polymers 33: 33-38, 1997 https://doi.org/10.1016/S0144-8617(97)00040-4
  14. Thomson RG. Granulation tissue and wound healing. In: General veterinary pathology, 2nd ed. Philadelphia: WB Saunders. 1984: 252-261
  15. Usami Y, Minami S, Okamoto Y, Matsuhashi A, Shigemasa Y. Influence of chain length of N-acetyl-D-glucosamine residues on direct and complement-mediated chemotactic activities for canine polymorphonuclear cells. Carbohydrate Polymers 32: 115-122, 1997 https://doi.org/10.1016/S0144-8617(96)00153-1
  16. Usami Y, Okamoto Y, Minami A, Kumazawa NH, Tanioka S, Shigemasa Y. Migration of canine neutrophils to chitin and chitosan. J Vet Med Sci 56: 1215-1216, 1994 https://doi.org/10.1292/jvms.56.1215
  17. Usami Y, Okamoto Y, Minami S, Matsuhashi A, Kumazawa NH, Tanioka S, Shigemasa Y. Chitin and chitosan induce migration of bovine polymorphonuclear cells. J Vet Med Sci 56: 761-762, 1994 https://doi.org/10.1292/jvms.56.761
  18. Yu Y, Park WH, Lee SC, Ko BM. Effects of chitooligosaccharide on the early stage ofwound healing. J Chitin Chitosan 8: 3539, 2003
  19. 구 영. 이온 빔 조사 처리된 키토산 스펀지의 세포적합도 에 관한 연구 대한치주과학회지 1998; 28: 281-29 1
  20. 검옥수, 정현주. 키토산이 배양중인 치주인대세포에 미치 는 영향. 대한치주과학회지 2001; 31: 163-180
  21. 김옥수, 정현주. 키토산이 치은섬유아세포에 미치는 영향. 대한치주과학회지 2002; 32: 235-247
  22. 이창균, 황정효, 이용무, 구영, 류인철, 이승진, 한수부, 최 상묵, 정종평. RGD 웹타이드로 표면개질된 키토산막의 생 물학적 영향. 대한치주과학회지 2003; 33: 27-35
  23. 정의원, 서종진, 최성호, 조규성, 채중규, 김종관. 백서 두 개골 결손부에서 키토산의 골조직 재생 유도 효과. 대한치주과학회지 2000; 30: 851-870