Effect of Osmolarity of Culture Medium on the Preimplantation Development of Porcine NT and IVF Embryos

  • 발행 : 2007.06.30

초록

In vitro development of porcine embryo is affected by culture condition. One possible factor is osmolarity of culture medium. 1his study examined whether high osmolarity of culture medium at the early culture stage improves development of preimplantation porcine in vitro fertilization (IVF) and nuclear transfer (NT) embryos. NT and IVF embryos were divided into three groups and the basic medium was PZM-3 ($250{\sim}270$ mOsmol, control group). The control group of embryos was cultured in PZM-3 for whole culture period. Other two groups of embryos were cultured in a modified PZM-3 with 0.05 M sorbitol or 0.05 M sucrose ($300{\sim}320$ mOsmol, sorbitol or sucrose group) for the first 2 days, and then cultured in PZM-3 for further culture. NT embryos cultured in sucrose group showed a significantly higher developmental rate to the blastocyst stage with a decreased apoptosis rate compared to the sorbitol (p<0.05). For IVF, sucrose group showed a significantly increased the blastocyst formation rate with a decreased apoptosis rate compared to the control (p<0.05). This study represents that the high osmolarity in the early embryo culture stage can enhance the in vitro development of porcine NT and IVF embryos to the blastocyst stage with reduced apoptosis of cells.

키워드

참고문헌

  1. Abeydeera LR, Wang WH, Cantley TC (1998a): Presence of epidermal growth factor during in vitro maturation of pig oocytes and embryo culture can modulate blastocyst development after in vitro fertilization. Mol Reprod Dev 51:395-401 https://doi.org/10.1002/(SICI)1098-2795(199812)51:4<395::AID-MRD6>3.0.CO;2-Y
  2. Abeydeera LR, Wang WH, Cantley TC, Ridke A, Day BN (1998b): Co-culture with follicular shell pieces can enhance the developmental competence of pig oocytes after in vitro fertilization: relevance to intracellular glutathione. Biol Reprod 58:213-218 https://doi.org/10.1095/biolreprod58.1.213
  3. Abeydeera LR, Wang WH, Cantley TC, Rieke A, Murphy CN, Prather RS, Day BN (2000): Development and viability of pig oocytes matured in a protein-free medium containing epidermal growth factor. Theriogenology 54:787-797 https://doi.org/10.1016/S0093-691X(00)00390-3
  4. Andrew JK, Abeydeera LR, Alvarez IM, Day BN, Buhi WC (2000): Effects of the porcine oviductspecific glycoprotein on fertilization, polyspermy, and embryonic development in vitro. Biol Reprod 63: 242-250 https://doi.org/10.1095/biolreprod63.1.242
  5. Bavister BD, Leibfried ML, Lieberman G (1983): Development of preimplantation embryos of the golden hamster in a defined culture medium. Biol Reprod 28:235-247 https://doi.org/10.1095/biolreprod28.1.235
  6. Betthauser J, Forsberg E, Augenstein M, Childs L, Eilertsen K, Enos J, Forsythe T, Golueke P, Jurgella G, Koppang R, Lesmeister T, Mallon K, Mell G, Misica P, Pace M, Pfister-Genskow M, Sterlchenko N, Voelker G, Watt S, Thompson S and Bishop M (2000): Production of cloned pigs from in vitro systems. Nat Biotechnol 18:1055-1059 https://doi.org/10.1038/80242
  7. Brinster RL (1965): Study on the development of mouse embryos in vitro I : the effect of osmolarity and hydrogen ion concentration. J Exp Zool 158:49-58 https://doi.org/10.1002/jez.1401580105
  8. Byrne AT, Southgate J, Brison DR, Leese HJ (1999): Analysis of apoptosis in the preimplantation bovine embryo using TUNEL. J Reprod Fertil 117:97-105 https://doi.org/10.1530/jrf.0.1170097
  9. Feugang JM, Roover DE, Leonard S, Dessy F, Donnay I (2002): Kinetics of apoptosis in preimplantation bovine embryos produced in vitro and in vivo. Theriogenology 57:494
  10. Funahashi H, Kim NH, Stumpf TT, Cantley TC, Day BN (1996): Presence of organic osmolytes in maturation medium enhances cytoplasmic maturation of porcine oocytes. Biol Reprod 54:1412-1419 https://doi.org/10.1095/biolreprod54.6.1412
  11. Gandhi AP, Lane M, Gardner DK, Krisher RL (2001): Substrate utilization in porcine embryos cultured in NCSU23 and G1.2/G2.2 sequential culture media. Mol Reprod Dev 58:269-275 https://doi.org/10.1002/1098-2795(200103)58:3<269::AID-MRD4>3.0.CO;2-L
  12. Gjorret JO, Wengle J, King WA, Schellander K, Hyttel P (2002): Occurrence of apoptosis in bovine embryos reconstructed by nuclear transfer or derived in vivo. Theriogenology 57:495
  13. Hardy K, Handyside AH, Winston RML (1989): The human blastocyst: cell number, death and allocation during late preimplantation development in vitro. Development 107:597-604
  14. Hardy K, Spanos S, Becker D, Iannelli P, Winston RM, Stark J (2001): From cell death to embryo arrest: mathematical models of human preimplantation embryo development. Proc Natl Acad Sci USA 98: 1665-1660
  15. Im GS, Lai L, Liu Z, Hao Y, Wax D, Bonk A, Prather RS (2004): In vitro development of preimplantation porcine nuclear transfer embryos cultured in different media and gas atmospheres. Theriogenology 61:1125-1135 https://doi.org/10.1016/j.theriogenology.2003.06.006
  16. Im GS, Yang BS, Lai L, Liu Z, Hao Y, Prather RS (2005): Fragmentation and development of preimplantation porcine embryos derived by parthenogenetic activation and nuclear transfer. Mol Reprod Dev 71(2):159-165 https://doi.org/10.1002/mrd.20258
  17. Jurisicova A, Latham KE, Casper RF, Varmuza SL (1998): Expression and regulation of genes associated with cell death during murine pre-implantation embryo development. Mol Reprod Dev 51: 243-253 https://doi.org/10.1002/(SICI)1098-2795(199811)51:3<243::AID-MRD3>3.0.CO;2-P
  18. Kano K, Miyano T, Kato S (1998): Effects of glycosaminoglycans on the development of in vitromatured and -fertilized porcine oocytes to the blastocyst stage in vitro. Biol Reprod 58:1226-1232 https://doi.org/10.1095/biolreprod58.5.1226
  19. Kim MK, Kwon DJ, Park CK, Yang BK (2005): Effects of culture medium and osmolarity on in vitro maturation of follicular oocytes and development of parthenogenetic embryos in porcine. Reprod Dev Biol 29:169-174
  20. Levy RR, Cordonier H, Czyba JC, Goerin JF (2001): Apoptosis in preimplantation mammalian embryo and genetics. Int J Anat Embryol 106 (suppl) 2:101-108
  21. Liu L, Trimarchi JR, Keef DL (2002): Haploid but parthenogenetic activation leads to increased incidence of apoptosis in mouse embryos. Biol Reprod 66:204-210 https://doi.org/10.1095/biolreprod66.1.204
  22. Mercola M, Stiles CD (1998): Growth factor superfamilies and mammalian embryogenesis. Development 102:451-460
  23. Mckiernan SH, Bavister BD (1990): Environmental variables influencing in vitro development of hamster 2-cell embryos to the blastocyst stage. Biol Reprod 43:404-413 https://doi.org/10.1095/biolreprod43.3.404
  24. Miyoshi K, Funahashi H, Okuda K, Niwa K (1994): Development of rat one-cell embryos in a chemically defined medium: effects of glucose phosphate and osmolarity. J Reprod Fertil 100:21-26 https://doi.org/10.1530/jrf.0.1000021
  25. Naglee DL, Maurer RA, Foote HR (1969): Effect of osmolarity on in vitro of rabbit embryos in a chemically defined medium. Exp Cell Res 58:331-333 https://doi.org/10.1016/0014-4827(69)90513-8
  26. Neuber E, Luetjens CM, Chan AWS, Schatten GP (2002): Analysis of DNA fragmentation of in vitro cultured bovine blastocysts using TUNEL. Theriogenology 57:2193-2202 https://doi.org/10.1016/S0093-691X(02)00901-9
  27. Nguyen VT, Kure-bayashi S, Harayama H, Nagai T, Miyake M (2003): Stage specific effects of the osmolarity of a culture medium on the development of parthenogenetic diploids in the pig. Theriogenology 59:719-734 https://doi.org/10.1016/S0093-691X(02)01085-3
  28. Onishi A, Iwamoto M, Akita T, Mikawa S, Takeda K, Awata T, Hanada H, Perry ACF (2000): Pig cloning by microinjection of fetal fibroblast nuclei. Science 289:1188-1190 https://doi.org/10.1126/science.289.5482.1188
  29. Petters RM, Johnson BH, Reed ML, Archibong AE (1990): Glucose, glutamine and inorganic phosphate in early development of the pig embryo in vitro. J Reprod Fertil 89:269-275 https://doi.org/10.1530/jrf.0.0890269
  30. Wang WH, Abeydeera LR, Prather RS, Day BN (2000): Actin filament distribution in blocked and developing pig embryos. Zygote 8:353-358 https://doi.org/10.1017/S0967199400001143
  31. Walker SC, Shin TY, Zaunbrecher GM, Romano JE, Johnson GA, Bazer FW, Piedrahita JA (2002): A highly efficient method for porcine cloning by nuclear transfer using in vitro-matured oocytes. Cloning Stem Cells 4:105-112 https://doi.org/10.1089/153623002320253283
  32. Whitten WK (1971): Nutrient requirements for the culture of preimplantation embryos in vitro. In: Raspa G (ed) Advances in the Bioscience. Oxford: Pergamon Press 6:129-141