Nutrition Research and Practice

The Korean Nutrition Society (한국영양학회)
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Effect of mucilage from yam on activation of lymphocytic immune cells

  • Jang, Cheol-Min (School of Bioresource Science, Andong National University) ;
  • Kweon, Dae-Hyuk (Department of Genetic Engineering, Sungkyunkwan University) ;
  • Lee, Jong-Hwa (School of Bioresource Science, Andong National University)
  • Published : 2007.06.20
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Abstract

The immunostimulating activities of mucilage fraction from yam were investigated. The proliferation of BSA-primed lymph node cells was enhanced between 4.1- to 10.9-fold compare to control, when cultured with 1 to $25{\mu}g/mL$ of yam-mucilage fraction. It showed strong immunostimulating activity than ginseng extract and as remarkable as Bifidobacterium adolescentis M101-4 known as a positive immunostimulator. Mitogenicity to lymph node cells was fully induced by concanavalin A and lipopolysaccharide. The proliferation of splenocytes and Peyer's patch cells was enhanced between 5.0- to 14.1-fold and 2.4- to 6.4-fold, respectively, when cultured with 1 to $25{\mu}g/mL$ of yam-mucilage fraction. It enhanced the production of cytokines such as tumor necrosis $factor-{\alpha}$ and IL-6 in the culture of RAW 264.7 macrophage cells. In the culture of lipopolysaccharide-stimulated RAW 264.7 cells, production of cytokines was as similar as compared to controls. In unstimulated RAW 264.7 cells, both tumor necrosis $factor-{\alpha}$ and IL-6 production were enhanced between 15.6- to 60.1-fold and 2.3- to 9.1-fold, respectively. Mucilage fraction from yam is expected to be a safe immunopotentiator to maintain the host immunity and develop a physiologically functional food.

Keywords

References

  1. Chihara G, Hamuro J, Maeda YY, Shiio T, Suga T, Takasuka N & Sasaki T (1987). Antitumor and metastasis-inhibitory activities of lentinan as an immunomodulator: an overview. Cancer Detect Prev 1:423-443
  2. Choi IS, Lee IS & Ku SJ (1992). The study on rheological and thermal properties of Dioscorea batatas DECAISNE starch. Korean Journal of Food and Cookery Science 8:57-63
  3. Damais C, Bona C, Chedid L, Fleck J, Nauciel C & Martin JP (1975). Mitogenic effect of bacterial peptidoglycans possessing adjuvant activity. J Immunol 115:268-271
  4. Dong W, Azcona-Olivera JI, Brooks KH, Linz JE & Pestka JJ (1994). Elevated gene expression and production of interleukins 2, 4, 5, and 6 during exposure to vomitoxin (deoxynivalenol) and cycloheximide in the EL-4 thymoma. Toxicol Appl Pharmacol 127:282-290 https://doi.org/10.1006/taap.1994.1163
  5. Ha YD, Lee SB & Kwak YG (1998). Removal of heavy metal and ACE inhibition of yam mucilage. Journal of the Korean Society of Food Science and Nutrition 27:751-755
  6. Han YN, Han SH & Lee IR (1990). Purification of mucilages from Dioscorea batatas and D. japonica and their content analysis. Korean Journal of Pharmacognosy 21:274-283
  7. Jung SK, Jung YY & Jung WS (1996). Studies on the browning inhibition of yam (Dioscorea aimadoimo) during hot air dehydration. Journal of the Korean Society of Agricultural Chemistry and Biotechnology 39:384-388
  8. Kim HS, Kim SS, Park YG & Seog HM (1991). Physicochemical properties of several Korean yam starches. Korean Journal of Food Science and Technology 23:554-560
  9. Kim YS, Lee BE, Kim KJ, Lee YT, Cho BK & Chung YC (1998). Antitumor and immunomodulatory activities of the P. grandiflorum cultivated for more than 20 years. Yakhak Hoeji 42:382-387
  10. Kwon EG, Choe EM & Gu SJ (2001a). Effects of mucilage from Yam (Dioscorea batatas DECENE) on blood glucose and lipid composition in alloxan-induced diabetic mice. Korean Journal of Food Science and Technology 33:795-801
  11. Kwon J, Lee SJ, So JN & Oh CH (2001b). Effects of Schizandra chinensis fructus on the immunoregulatory action and apoptosis of L1210 cells. Korean Journal of Food Science and Technology 33:384-388
  12. Kwon JH, Lee KD, Lee SJ, Jung SK & Choi JW (1998). Changes in chemical components and physical properties with freeze drying and hot air-drying of Dioscorea batatas. Journal of the Korean Society of Food Science and Nutrition 27:908-913
  13. Lee BY & Kim HG (1998). Quality properties of korean yam by various drying methods. Korean Journal of Food Science and Technology 30:877-882
  14. Lee BY, Lee YC, Kim HM, Kim CJ & Park MH (1992). Rheological properties of the gelatinized yam starch solution. Korean Journal of Food Science and Technology 24:619-622
  15. Lee BY, Park DJ, Ku KH, Kim HG & Mok CK (1994). Mucilage separation of Korean yam using microparticulation / air classification process. Korean Journal of Food Science and Technology 26:596-602
  16. Lee CB (1982). IIIustrated of Flora of Korea, p.225. Hyung-Moon Publishing Co., Seoul. Republic of Korea
  17. Lee HY & Lee HS (1998). Stimulatory effect of Korean red-ginseng extract on the proliferation and cellular activity of lymphocytes. Journal of Ginseng Research 22:60-65
  18. Lee IM (1996). Characteristics and functionalities of biologically active substances from yam (dioscorea batatas DECENE). Kyung Hee University
  19. Lee J, Ametani A, Enomoto A, Sato Y, Motoshima H, Ike F & Kaminogawa S (1993). Screening for the immunopotentiation activity of food microorganisms and enhancement of the immune response by Bifidobacterium adolescentis M101-4. Biosci Biotechnol Biochem 57:2127-2132 https://doi.org/10.1271/bbb.57.2127
  20. Lim SD, Seong KS, Kim KS & Han DU (2007). Effects of fermented milk containing herb extract from Acanthopanax divaricatus var. albeofructus and Codonopsis Ianceolata on the immune status of mouse. Korean Journal for Food Science of Animal Products 27:95-101 https://doi.org/10.5851/kosfa.2007.27.1.95
  21. Medicine Botany Society for the Research (1993). New Medicine Botany, p.228-229. Hak-Chang Publishing Co., Seoul. Republic of Korea
  22. Misaki A, Ito T & Harada T (1972). Constitutional studies on the mucilage of "Yamanoimo" Dioscorea batatas Decne, forma Tsukune. Agricultural and Biological Chemistry 36:761-771
  23. Mosmann T (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 16:55-63
  24. Ohtani K & Murakami K (1991). Structure of mannan fractionated from water-soluble mucilage Nagaimo (Dioscorea batatas DECNE). Agricultural and Biological Chemistry 55:2413-2414
  25. Sekine K, Watanabe-Sekine E, Toida T, Kawashima T, Kataoka T & Hashimoto Y (1994). Adjuvant activity of the cell wall of Bifidobacterium infantis for in vivo immune responses in mice. Immunopharmacol Immunotoxicol 16:589-609 https://doi.org/10.3109/08923979409019741
  26. Sminia T, Wilders MM, Janes EM & Hoefsmit EC (1983). Characteristics of non-lymphoid cells in Peyer's patches of the rat. Immunobiology 164:136-143 https://doi.org/10.1016/S0171-2985(83)80005-9
  27. Suzuki I, Hashimoto K & Yadomae T (1990). Rapid preparation of functional murine Peyer's patch cells. Bioscience Microflora 9:87-98
  28. Tanoue H & Simozono H (1991). Chemical and rheological properties of viscous polysaccharides from three species of yam (Dioscorea). Nippon Shokuhin Kyogo Gakkaishi 38:751-757 https://doi.org/10.3136/nskkk1962.38.751
  29. Tomoda M, Ishikawa K & Yokoi, M (1981). Plant mucilages. XXX. isolation and characterization of a mucilage, "Dioscorea-mucilage B" from the rhizophors of Dioscorea batatas. Chem Pharm Bull 29:3256-3261 https://doi.org/10.1248/cpb.29.3256