Antijuvenoid Action of Terpenoid Imidazole Compound on Larval - Pupal - Adult Development of Silkworm, Bombyx mori L.

  • Published : 2007.06.30

Abstract

Precocious metamorphosis was induced in two popular bivoltine breeds i.e. YB and $NB_4D_2$ by an imidazole compound having anti-juvenile hormone activity. The chemical was administered by feeding treatment with mulberry leaf to freshly moulted (0-6 hrs) IV instar larvae. The dose of the chemical was found to be breed specific being 650 ppm for YB and 500 ppm for $NB_4D_2$. The chemical caused complete skipping up of the fifth instar larvae which is most susceptible to diseases. But IV instar was somewhat prolonged. As a result the total larval period was reduced by 4-5 days. However, some of the rearing and reeling parameters like cocoon yield, cocoon weight, shell weight, shell %, filament length and fecundity were reduced for that particular generation only. The effective rate of rearing (ERR %) was significantly increased in trimoulters during the most unfavourable August - September seed crop season. Number of cocoons / kg and number of male moth were significantly higher in trimoulters. These may be useful for preparation of $multi{\times}bi$ hybrid seed. Fine denier was also found in trimoulter cocoon which is the cause of getting fine silk filament from trimoulter cocoons. Normal mating behaviour and emergence pattern was recorded in trimoulters. Bivoltine trimoulter males also showed competence for mating with multivoltine females. These results suggest the possibility of getting trimoulter males during hot and humid seasons when rearing of bivoltine is almost impossible particularly in Eastern and North Eastern India.

Keywords

References

  1. Akai, H. (1988) Hormonal regulation of larval development and its utilization in silk production by Bombyx silkmoth. JARQ 22(2), 128-134
  2. Akai, H., K.Kimura, M. Kiuchi, and A. Shibukawa (1984) Effect of anti-juvenoid treatment on cocoon and cocoon filaments in Bombyx mori L. J. Seric. Sci., Jpn. 53, 545-546
  3. Akai, H., M Kiuchi, and K. Kimura (1986) Effect of anti-JH treatment on the size and fine structure of cocoon filament of B. mori J. Seric. Sci., Jpn. 55, 388-396
  4. Akai, H. and B. Mauchamp (1989). Supperssive effects on an Imidazole derivative, KK-42 on JH levels in hemolymph of Bombyx larvae. J. Seric. Sci. Jpn., 58(1), 73-74
  5. Asano, S., E. Kuwano and M. Eto (1984a) Anti juvenile hormone activity of l-citronellyl-5-phenylimidazole in the $3^{rd}$ instar silkworm, Bombyx mori L. (Lepidoptera,Bombycidae). Appl. Ent. Zool. 19, 212-220 https://doi.org/10.1303/aez.19.212
  6. Asano, S., E. Kuwano, and M. Eto (1984b) Induction of precocious metamorphosis by dietary administration of l-citronellyl-5-phenyllmidazole in the $4^{th}$ instar larvae of the silkworm, Bombyx mori L. (Lepidoptera, Bombycidae). Appl. Ent. Zool. 21, 63-69
  7. Asano, S., E. Kuwano and M. Eto (1987).Threshold size for precocious metamorphosis by an anti-juvenile hormone in silkworm larvae. Bombyx mori L .Appl. Ent.. Zool. 22(4), 424-433 https://doi.org/10.1303/aez.22.424
  8. Banerjee, K. (1998) Reproductive performance and cocoon characters of Bombyx mori L with reference to regulated feeding and treatments with methoprene and imidazole compounds. Ph D Thesis; University of North Bengal, India
  9. Banerjee, K. and D. C. Deb (1999) Effects of KK-42, an imidazole derivative, on Rearing and Reproduction performance of Bombyx mori L. during the hot, wet season. Insect Sci. Applic. 19(1), 85-90
  10. Ding Nong, Z. X., M.Q. Jiang, H.W. XU, Z. Wang and M.K Ku (1991) Studies on the ditary efficiency of the silkworm Bombyx mori L. on the physiological factors and the leaf - silk ratio. Sci. Seri., 17(4), 193-199
  11. Gu, S. H., Y. Iwashita and H. Kawasaki (1988), Endocrinological changes of trimoulter silkworms induced by an Imidazole compound. J. Seric. Sci., Jap. 57(3), 210-215
  12. Gu, S. H., Y. S. Chow (1996) Regulation of juvenile hormone biosynthesis by ecdysteriod levels during the early stages of the last two larval instars of Bombyx mori. Journal of Insect Physiology 42, 625-632 https://doi.org/10.1016/0022-1910(96)00025-X
  13. Gu, S. H., Y. S. Chow (1997). Analysis of precocious metamorphosis induced by application of an Imidazolederivative to early third instar larval of the silkworm B.mori. Archives of Insect Bio-chemistry Physiology 36, 349-361 https://doi.org/10.1002/(SICI)1520-6327(1997)36:4<349::AID-ARCH7>3.0.CO;2-M
  14. Hemantharaj M. T., Yungen Miao and Junliang. Xu (1996) Studies on the effect of AJH and MH on the larval development, cocoon characters and silk quality in silkworm Bombyx mori L. J. Zhejiang Agric. Univ. 22(6), 566
  15. Hemantharaj M. T., R. Meenal, R. Singh and R. K. Rajan (1999) Anti- Juvenile hormones and their application in sericulture for quality silk production. Bull. Sericult. Res. 10, 1-8
  16. He, Y. ands. He (1994)- Application of inducing trimoulters in breeding Bombyx mori Canye Kexue, 20(1), 30-34
  17. Iwashita, Y. (1963) Studies on the relationships between changes in moultinism and changes in some characters and morphology in the silkworm, Bombyx mori L. Mem. Coll. Agric. Utsnomiya Univ., 17, 1-93
  18. Kadono-okuda, K., E. Kuwano, M. Eto and A. Yamashita (1987) Inhibitory action of an imadizole compound on ecdysone synthesis in prothoracic glands of the silkworm Bombyx mori . Dev Growth Differ 29, 527-533 https://doi.org/10.1111/j.1440-169X.1987.00527.x
  19. Kataoka, K., T. Imai, M. Kiuchi and H. Akai (1985) Physical properties of filaments of trimoulters induced by anti-juvenoid in Bombyx mori. Seric. Sci. Jpn. 54(5), 377-381
  20. Kawaguchi,Y., S. Akagi, K. Koga, H. Fujii and E. Kuwano (1993) Egg formation in KK-42 induced trimoulter of B. mori. Sci. Bull. Fac.Agric.Kyushu Univ. 47, 1-6
  21. Kimura, K., M. Kiuchi and H. Akai (1986) Effects of JH analogue and anti-JH treatments on the number and size of silkworm eggs J. Seric. Sci. Jpn, 55(4), 35-337
  22. Kimura, K., M. Kiuchi and H Akai (1986a) Effect of juvenile hormone analogue and anti-jh treatments on the number and size of the silkworm eggs. J. Seric. Sci. Jpn, 55(4), 335-337
  23. Kiuchi, M., S. Abe and H. Akai (1986) Growth and food efficiency in precocious trimoulter silkworms induced by administration of an anti- JH compound. J. Seric. Sci. Jpn, 55, 246-251
  24. Kiuchi, M. and H. Akai (1988) In vivo and in vitro inhibition of prothoracic gland activity by 1-benzyl-5-[(E)-2,6-dimethyl-1,5-heptadienyl] imidazole (KK-42) in Bombyx mori. In, Kuroda, E., Kurstak, E., Maramorosch, K. (Eds). Invertebrate and Fish Tissue Culture. Japan Scientific Societies Press/pringer- Verlag. Tokyo/Barlin, pp. 60-63
  25. Kiuchi, M., K. Kimura. And H. Akai (1985). Induction of trimoulters from a tetramoulter strain of B.mori by anti-juvenoid treatment. J. Seric. Sci. Jpn., 54(1) 77-81
  26. Krishnaswami, S. (1986b) New Technology of Silkworm Rearing. Bulletin No.2, Central Silk Board Publication, pp. 1- 23
  27. Kuwano, E. and M. Eto (1983) Terpenoid imidazoles, New anti-juvenile hormones and insecticidal compounds. Proc. $10^{th}$ Int. congr. Plant Prot. Brighton, 1, 278
  28. Kuwano, E., R.Takeya and M. Eto (1983), Agric. Biol. Chem., 47, 921-923 https://doi.org/10.1271/bbb1961.47.921
  29. Kuwano, E., R. Takeya and M. Eto (1984). Synthesis and anti-juvenile hormone activity of 1-citronellyl-5-substituted imidazoles. Agric. Biol. Chem., 48(12), 3115-3119 https://doi.org/10.1271/bbb1961.48.3115
  30. Kuwano, E., R. Takeya and M. Eto (1985) Synthesis and anti-juvenile hormone activity of 1-substituted-5-L(E)-2,6-dimethyl-1,5 heptadienyl imidazoles Agric. Biol. Chem. 49, 483-486 https://doi.org/10.1271/bbb1961.49.483
  31. Kuwano, E., M. Kiuchi and M. Eto (1988) Induction of precocious metamorphosis by 1-5 disubstituted imidazoles with a non-terpene chain. Agric. Biol. Chem. 52, 1619-1620 https://doi.org/10.1271/bbb1961.52.1619
  32. Lu Xuefang. and Ronggi. Li (1987) Studies on the super fine silk production of induced trimoulting silkworm by SM-1. Sci. Seric. 13(2), 71-76
  33. Matsumura, S. and Y Takeuchi (1950) Experiment on the nutrition of silkworm Bombyx mori. J. Seric. Sci. Tokyo, 19, 192-200
  34. Miyajima, T., T. Yamamoto, K. Mase, T. Iizuka, M. Nozaki and M. Kiuchi, (2001) Induction of trimoulting larvae by the imidazole compound, triflumizole, in the silkworm races with a thin cocoon filament, Hakugin and Honobono, and the resulted cocoon characters. J.Seric. Sci. jpn. 70(1), 37-42
  35. Murakoshi, S. (1972) Jap. J. Appl. Ent, Zool., 16, III, In, The silkworm an important lab tool, Ed. Y. Tazima, Kodanshe, Tokyo
  36. Murakoshi, S. and L. Ichimoto (1972) Jap. J. Appl. Ent, Zool., 16, III, In, The silkworm an important lab tool, Ed. Y. Tazima, Kodanshe, Tokyo
  37. Murakoshi, S., T. Kamikado and S. Tamure (1977) Change in moltinism and integument colour of silkworm larvae, Bombyx mori L. by some quinolone alkaloids. Jap. J. appl. Ent Zool. 21, 230-232 https://doi.org/10.1303/jjaez.21.230
  38. Nagata, M. and J. Kobayashi (1990) Effect of nutrition on storage protein concentration in the larval haemolymph of the silkworm Bombyx mori L. J. Seric. Sci. Jpn., 59(6), 469-474
  39. Okuda, K. K., E. Kuwano, M. Eto, and O. Yamashita (1987) Anti-ecdysteriod action of some Imidazolederivatives on pupal-adult development of the silkworm, B.mori (Lepidoptera, Bombycidae). Appl. Ent. Zool. 22, 370-379 https://doi.org/10.1303/aez.22.370
  40. Riddiford L. M. (1980) Interaction of ecdysteroids and juvenile hormone in the regulation of larval growth and metamorphosis of the tobacco hornworm. In. Progress in Ecdysone Research (Ed. by Hoffmann J. A.), pp. 409-430. Elsevier/North - Holland, Amsterdam
  41. Sakurai, S. (1983) Temporal organization of endocrine events underlying larval ecdysis in the silkworm, Bombyx mori. J. Insect. Physiol., 29, 919-932 https://doi.org/10.1016/0022-1910(83)90055-0
  42. Staal, G. B., C. A. Henrick, B. J. Bergot, D. C. Cerf, J. P. Edwards and S. J. Kramer(1981) Relationship and interactions between JH and anti analogues in Lepidoptera. In Regulation of Insect Development and Behavior. Pr. Nauk. Inst. Chem. Org. Fiz. Politech-Wroclaw 20, 323-340
  43. Trivedy, K., R. K. Datta, O.K.Remadevi and S.B Magadum (1994) Effect of anti-juvenoied KK-42 on the silkworm Bombyx mori L. G. Ital. Entomol., 7, 123-139
  44. Tsukuda, M., K. Kimura, A Shibukawa,A. Shimazaki and H. Akai (1987) Effect of treatment with juvenile hormone analogue and anti- juvenoid on the size of the cocoon filament. J.Seric.Sci. Jpn., 56(3), 231-234
  45. Wigglesworth, V. B. (1985) Historical perspectives. In : Comprehensive Insect Physiology, Biochemistry and Pharmaclogy, Vol. 7, Endocrinology I, Kerkut, G. A. and Gilbert, L. T. (Eds.), Pergamon Press, Oxford, pp. 1-24
  46. Xu, Junliang, Lu. shunlin, Miao. Yuangen, Zhang. Chuanxi, Wu. Xiaofeng, Shi, Zuhua and Zhang Fangyue (1994). In silkworm physiology, editorial deparment, Bulletin of sericulture, Hangzhou, China. pp. 239-342
  47. Xue, Fenglu. (1992) the production of superfine cocoon filaments by insect growth regulators. Proceedings XIX International Congress of Entomology, Beijing, china, $28^{th}$ June - $4^{th}$ july: 650
  48. Xue, Fenglu., Y.C Wu and E.S. Wu (1990) Application of trimoulter inducer to silkworm egg production II. Induction of trimoulting silkworm for hybrid parent and propagation of their offspring. Canye Kexue 16(2), 65-70
  49. Xue, Fenglu., Zhang Xueming and Ye Xiuzheing (1994) Produce F-1 hybrid seed by inducing sex limited races to trimoulting. Canye Kexue 20(1), 60-61