International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
권호 표지

Cloning and Characterization of 6-Phosphogluconolactonase Gene in Silkworm Bombyx mori

  • Yang, HuaJun (Institute of Life Sciences, Jiangsu University) ;
  • Chen, KePing (Institute of Life Sciences, Jiangsu University) ;
  • Yao, Qin (Institute of Life Sciences, Jiangsu University) ;
  • Guo, ZhongJian (Institute of Life Sciences, Jiangsu University)
  • Published : 2007.06.30
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Abstract

As the genome of B. mori is available in GenBank and the EST database of B. mori is expanding, identification of novel genes of B. mori was conceivable by datamining techniques and bioinformatics tools. In this study, we used the in silico cloning method to get the 6-Phosphogluconolactonase (6PGL) gene of B. mori and analysed with bioinformatics tools. The result was confirmed by RT-PCR and prokaryotic expression. The 6PGL cDNA comtains a 702 bp ORF. The deduced protein has 233 amino acid residues, with the predicted molecular weight of 25946. 72 Da, isoelectric point of 5.41, and contains conserved NagB domains. This gene has been registered in GenBank under the accession number EF198104.

Keywords

References

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