Physiological Changes in Rooting Zone of Dwarf Apple Rootstocks (Malus domestica Borkh.) after Stem Etiolation Treatment

사과 왜성대목들의 줄기 황화처리에 따른 발근 부위의 생리적 변화

  • Published : 2007.06.30

Abstract

This work was conducted to investigate the important rooting factors through comparative analysis of a physiological differentiation after layering treatment using four apple rootstocks of different rooting abilities; M.26, M.9, O.3, and Mo.84. Mo.84 showed the highest rooting rate in from rootstocks, while O.3 was the lowest. Mo.84 also found to have the highest indole-3-acetic acid (IAA) content, although the fluctuation of IAA contents was not consistent with layering treatment. In contrast, abscisic acid (ABA) content of Mo.84 which showed highest rooting was lowest among rootstocks regardless of layering treatment. And ABA contents of all rootstocks were decreased after layering treatment than before layering treatment. O.3 which showed poor rooting rate revealed lowest in boron content. Carbohydrate/nitrogen (C/N) ratio of Mo.84 was the highest in all rootstocks. Therefor, we assumed that he IAA contents in layering treated rootstocks were not seemed to be a major rooting factor, but the changes in ABA contents and boron levels limit rooting in dwarf apple rootstocks.

본 시험은 주요 왜성대목인 M.26 과 M.9, 발근력이 낮은 O.3, 발근력이 높은 Mo.84 등 4 종류의 사과 대목 품종을 재료로 휘묻이 번식시 황화처리에 따른 발근 부위의 생리적 변화를 시기별로 각각 비교, 분석하여 부정근 발생에 관여하는 요인을 구명하고자 하였다. 휘묻이 번식시 대목별 발근율은 Mo.84가 가장 높았고, O.3가 가장 낮았다. 대목 별 휘묻이 전 후의 IAA함량은 황화 처리 전 발근력이 높은 Mo.84에서 가장 높았으나 휘묻이 일수가 경과할 수록 IAA 힘량 변화는 일정한 경향이 없었다. 반면 발근력이 기장 좋은 Mo.84의 ABA 함량은 휘묻이 처리 전 후 모두 가장 낮게 나타났다. 또한 휘묻이 처리전에 비하여 처리후에서 ABA 함량은 모든 대목 품종에서 감소하였다. Boron의 함량은 발근율이 낮은 O.3에서 가장 낮게 나타났으며, C/N율은 모든 대목들에서 발근율이 높은 Mo.84 대목이 가장 높게 나타났다. 그러므로, 대목의 휘묻이 처리에 따른 IAA 함량은 대목의 발근율에 미치는 주요한 요인이 아닌 것으로 판단되지만 ABA 함량과 boron의 수준은 사과왜성대 목들의 발근을 제한하는 요인으로 추정된다.

Keywords

References

  1. Christine, A. W. and O.P. Jones. 1991. Micropropagation of some cold-hardy dwarfing rootstock for apple. J. Hort. Sci. 66(1): 1-6
  2. Gaspar, T., C. Penel, F.J. Castillo, and G. Greppin. 1985. A two-step control of basic and acidic peroxidases and its significance for growth and development, Physiol. Plant. 64:418 https://doi.org/10.1111/j.1399-3054.1985.tb03362.x
  3. Goo Y.B. 1995. A study on mass propagation of Larix leptolepis gordon by cutting and its rooting mechanism. Ph.D. Thesis, Seoul Univ, Suwon
  4. Gorecki, R.S. 1979. The effect of an auxin(IBA), fungicide(captan) and wounding on the rooting of softwood apple(Malus Mill) cuttings. Acta Agrobotanica 32:223-232
  5. Greenwood, M.S. and G.P. Berlyn. 1973. Sucroseindol-3-acetic acid interactions on root regeneration by Pinus lambertiana embryo cuttings. Amer. Jour. Bot. 60(1):42-47 https://doi.org/10.2307/2441321
  6. Hansen, O.B. 1990. Rapid production of apple rootstocks by softwood cuttings. Scientia Hort., 42:277-287 https://doi.org/10.1016/0304-4238(90)90051-F
  7. Harrison-Murray, R.S. 1982, Etiolation of stock plants for improved rooting of cuttings: . Opportunities suggested by work with apple, in: 'Proc, Int. Plant Prop. Soc., 31 :386
  8. Hartmann, H.T., D.E. Kester, and F.T. Davies, Jr. 1990. Plant propagation principles and practices. 5th ed. Prentice Hall, Englewood cliffs, NJ, USA
  9. Karhu, S.T. and S.K. Ulvinen. 1995. The effect of different carbohydrates on the rooting of micropropagated apple shoots and their adaption after transplantation. Bulletin des Recherches Agrononiques de Gemgloux.30(1/2):87-101
  10. Kim, I.Y. 2004. Changes in calcium contents of leaf and fruit during growth, effect of foliar sprays of calcium on fruit quality of 'niitaka' pear. Ph. D. thesis, Kyungpook Univ, Daegu
  11. Kwon, S.I., K.R. Kim, H.Y. Kim, and M.J. Kim. 1999. Effect of various mixed-treatment by some growth regulators, sugars and inorganic matters with IBA on rooting in hardwood cuttings of M.26 apple rootstock. J. Kor. Soc. Hort. Sci. 40(4):447-450
  12. Lee K.H., I.J. Lee, S.W. Jang, and C.K. Sang. 2004. Growth and flowering responses and changes of gibberellins and abscisic acid contents by cold treatment and cultivation temperature on chrysanthemum cv. 'Daewhakang', J. Kor. Soc. Hort. Sci. 45:354-358
  13. Majumber, P.K. and B.H. Howard. 1973. The response of M.9 hardwood cuttings to various rooting inducing treatment. Report of East Mailing Research Station for 1972. 67-69
  14. Maynard, B.K. and Bassuk, N.L. 1988. Etiolation and banding effects on adventitious root formation, in: 'Adventitious Root Formation by Cuttings,' T.D. Davis, B.E. Haissig, and N. Sankhla, eds., Adv. in Plant Sci. Ser., vol 2, Dioscorides Press, Portland. p. 29
  15. Olieman, van der Meer, R.L.M. Pierik, and S. Reest. 1971. Effects of sugar, auxin, and light on adventitious root formation in isolated stem explants of Phaseolus and Rhododendron. Med. Facult. Landbouw, Weten. Gent. 36(1):511-18
  16. Osborne, R.H. 1983. Propagation by softwood cuttings from root pieces to reintroduce juvenility in a new dwarf rootstock (Ottawa 3). Proc. Intl. Plant Prop. Soc. 33:301-365
  17. Seo, H.S. 1983. Study on cutting propagation of apple. Annual Report of Horticultural Experiment Station p. 245-247
  18. Shawky, I., H.M. El-Hennawy and H.F. El-Wakeel. 1993. Effect of IBA and cold storage on rooting of hardwood cuttings of MM.106 apple rootstock. Annals Agric. Sci., Air Shams Univ., Cairo, 38(2): 683-690
  19. Son, M.R. 1998. Application of near infrared spectroscopy for quality evaluation of apple fruit. Ph.D. Thesis, Kyungpook, Daegu
  20. Thimann, K.V. and F.W. Went. 1934. On the chemical nature of the root-forming hormones. Proc. Kon. Akad. Wetensch. Amsterdam. 37;456-459
  21. Vasek, J. and B.H. Howard. 1984. Effects of selective and biennial havesting on the production of apple stoolbeds. J. Hort. Sci. 59:477-485
  22. Wen-Quan S. and N.L. Bassuk. 1991. Stem banding enhances rooting and subsequent growth of M.9 and MM.106 apple rootstock cuttings. Hort. Sci. 26(11): 1368-1370
  23. Yoo, Y.K. and K.S. Kim. 1996. Seasonal variation in rooting ability, plant hormones, carbohydrate, nitrogen, starch and Soluble Sugar contents in cuttings of white forsythia. J. Kor. Soc. Hort. Sci. 37(4):554-560
  24. Tsugawa, C. 1984. The cultivation technology of apple. Yokendo (in Japan). p. 390-391