Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Cytoprotective Activity of Carpinus tschonoskii against H$_2$O$_2$ Induced Oxidative Stress

  • Zhang, Rui (Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University) ;
  • Kang, Kyoung-Ah (Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University) ;
  • Piao, Mei-Jing (Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University) ;
  • Park, Jae-Woo (Department of Nuclear and Energy Engineering and Applied Radiological Science Research Institute, Cheju National University) ;
  • Shin, Taek-Yun (Department of Veterinary Medicine, Cheju National University) ;
  • Yoo, Byoung-Sam (Cosmetic R&D Center, COSMAX Inc.) ;
  • Yang, Young-Taek (Jeju-do Agricultural Research & Extension Services) ;
  • Hyun, Jin-Won (Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University)
  • Published : 2007.06.30
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Abstract

We have studied the cytoprotective effect on H$_2$O$_2$ induced oxidative stress from leaves of Carpinus tschonoskii. The methanol extract of Carpinus tschonoskii was found to scavenge intracellular reactive oxygen species (ROS) using flow cytometry and confocal microscope. This extract prevented lipid peroxidation and thus reduced cell death of Chinese hamster lung fibroblast (V79-4) induced by H$_2$O$_2$ treatment. The extract increased catalase activity and phosphorylation of extracellular signal regulated kinase (ERK). Taken together, the results suggest that Carpinus tschonoskii protects V79-4 cells against oxidative damage by H$_2$O$_2$ through scavenging ROS.

Keywords

References

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