Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Cytoprotective Activity of Belamcanda chinensis Rhizome Against Glutamate-Induced Oxidative Injury in HT22 Cells

  • Jeong, Gil-Saeng (X-ray Microscopy Research Center, Wonkwang University) ;
  • An, Ren-Bo (College of Pharmacy, Wonkwnag University, College of Pharmacy, Yanbian University) ;
  • Oh, Seung-Hwan (Central R&D Center, Kwang Dong Pharmaceutical Co.) ;
  • Kang, Dae-Gill (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee, Ho-Sub (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Kim, Youn-Chul (College of Pharmacy, Wonkwang University)
  • Published : 2007.06.30
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Abstract

Four isoflavones including tectorigenin (1), irisflorentin (2), irigenin (3), and tectoridin (4) have been isolated from the 70% EtOH extract of Belamcanda chinensis rhizome. The evaluation for protective effect of compounds 1-4 against glutamate-induced cytotoxicity in hippocampal HT22 cell line was conducted. Compound 1 showed significant protective effect with an EC$_{50}$ value of 67.25 ${\pm}$ 1.2 ${\mu}$M, whereas compounds 2-4 were inactive. These results suggest that compound 1 may possess the neuroprotective activity against oxidative cellular injures.

Keywords

References

  1. Behl, C., Davis, J.B., Lesley, R., and Schubert, D., Hydrogen peroxide mediates amyloid b protein toxicity. Cell 77, 817-827 (1994) https://doi.org/10.1016/0092-8674(94)90131-7
  2. Colye, J.T. and Puttfarcken, P., Oxidative stress, glutamate and neurodegenerative disorders. Science 262, 689-695 (1993) https://doi.org/10.1126/science.7901908
  3. Davis, J.B. and Maher, P., Protein kinase C activation inhibits glutamateinduced cytotoxicity in a neuronal cell line. Brain Res. 652, 169-173 (1994) https://doi.org/10.1016/0006-8993(94)90334-4
  4. Eu, G.H., Woo, W.S., Chung, H.S., and Woo, E.H., Isoflavonoids of Belamcanda chinensis (II). Kor. J. Pharmacogn. 22, 13-17 (1991)
  5. Grundman, M., Grundman, M., and Delaney, P., Antioxidant strategies for Alzheimer's disease. Proc. Nutr. Soc. 61, 191-202 (2002)
  6. Kang, T.H., Baek, H.Y., and Kim, Y.C., Protective effect of Jakyak- Gancho-Tang extract and its constituents against t-BHP-induced oxidative damage in HT22 cells. Am. J. Chin. Med. 33, 181-189 (2005) https://doi.org/10.1142/S0192415X05002850
  7. Lee, H.U., Bae, E.A., and Kim, D.H., Hepatoprotective effect of tectoridin and tectorigenin on tert-butyl hydroperoxide-induced liver injury. J. Pharmacol. Sci. 97, 541-544 (2005) https://doi.org/10.1254/jphs.SCZ040467
  8. Lee, K.T., Sohn, I.C., Kim, D.H., Choi, J.W., Kwon, S.H., and Park, H.J., Hypoglycemic and hypolipidemic effects of tectorigenin and kaikasaponin III in the streptozotocin-induced diabetic rat and their antioxidant activity in vitro. Arch. Pharm. Res. 23, 461-466 (2000) https://doi.org/10.1007/BF02976573
  9. Lee, S.O., Woo, W.S., Woo, E.H., and Kim, K.S., Isoflavonoids of Belamcanda chinensis. Kor. J. Pharmacogn. 20, 219-222 (1989)
  10. Maher, P. and Davis, J.B., The role of monoamine metabolism in oxidative glutamate toxicity. J. Neurosci. 16, 6394-6401 (1996) https://doi.org/10.1523/JNEUROSCI.16-20-06394.1996
  11. Makkesbery, W.R., Oxidative stress hypothesis in Alzhiemer's disease. Free Radical Bio. Med. 23, 134-147 (1997) https://doi.org/10.1016/S0891-5849(96)00629-6
  12. Mosmann, T., Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods 65, 55-63 (1983) https://doi.org/10.1016/0022-1759(83)90303-4
  13. Park, E.K., Shin, Y.W., Lee, H.U., Lee, C.S., and Kim, D.H., Passive cutaneous anaphylaxis-inhibitory action of tectorigenin, a metabolite of tectoridin by intestinal microflora. Biol. Pharm. Bull. 27, 1099-1102 (2004) https://doi.org/10.1248/bpb.27.1099
  14. Shin, K.H., Kim, Y.P., Lim, S.S., Lee, S., Ryu, N., Yamada, M., and Ohuchi, K., Inhibition of prostaglandin E2 production by the isoflavones tectorigenin and tectoridin isolated from the rhizomes of Belamcanda chinensis. Planta Med. 65, 776-777 (1999) https://doi.org/10.1055/s-2006-960868
  15. Simonian, N.A. and Coly, J.T., Oxidative stress in neurodegenerative diseases. Ann. Rev. Pharmacol. 36, 83-106 (1996) https://doi.org/10.1146/annurev.pa.36.040196.000503
  16. Zhu, Y.-P., Chinese Materia Medica, Harwood Academic Publishers, Amsterdam, 1998