Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Plant Regeneration through Leaf Explant Culture of Boxthorn (Lycium chinense Mill.)

  • Ham, In-Ki (Chungcheongnam-do Agricultural Research & Extension Services) ;
  • Park, Sang-Kyu (Chungcheongnam-do Agricultural Research & Extension Services) ;
  • Lee, Bong-Chun (Chungcheongnam-do Agricultural Research & Extension Services) ;
  • Lee, Mi-Ae (Chungcheongnam-do Agricultural Research & Extension Services) ;
  • Kwon, Kyeong-Hak (Chungcheongnam-do Agricultural Research & Extension Services) ;
  • Lee, Eun-Mo (Chungcheongnam-do Agricultural Research & Extension Services) ;
  • Jo, Man-Hyun (Chungcheongnam-do Agricultural Research & Extension Services)
  • Published : 2007.06.30
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Abstract

This study was conducted to investigate the optimal plant growth regulator level for the shoot formation of Lycium chinense Mill. In vitro plant propagation was developed for leaf explants of boxthorn. Leaf explants were cultured on MS medium supplemented with cytokinins (BA and 2-iP) alone. Plants were successfully regenerated through in vitro culture by using leaf explants of boxthorn grown in the field. After 4 weeks of culture, 58% of shoot formation had developed from the leaf explants. The shoot formation rate of 'Jangmyeong' was highest followed by 'Myeongan', ;Cheondae', and 'Bullo'. The use of 0.2mg/L BA was critical for enhanced production of shoot formation and resulted in 58% of the culture producing shoot formations. Regenerated plantlets transplanted to pots were developed and successfully acclimatized to greenhouse.

Keywords

References

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