Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Heterochromatic Knob Number And Karyotype in Korean Indigenous Waxy Corn by Giemsa C-banding Pattern of Mitotic Chromosome

C-banding 패턴에 의한 한국 재래종 찰옥수수 염색체의 Heterochromatic knob 수와 핵형

  • Published : 2007.06.25
Download PDF
⟨ Previous Next ⟩

Abstract

A Giemsa C-banding method was used for the identification of somatic chromosomes and heterochromatic knob position in Korean indigenous waxy com (Zea mays L.). 5 inbred stocks were examined and their heterochromatic knob numbers ranged from 6 to 12. In comparison of homologous chromosomes of two stocks of YS-1 and MY-1, knob numbers, knob positions, arm ratios and relative length of chromosomes were different between the genotypes. The length of homologous chromosomes in YS-1 were generally larger than those of MY-1. The Giemsa method was proved to be useful for the identification of somatic chromosome and a C-banded diagram showing knob positions, arm ratios and relative length of chromosome could be used as a good tool to compare the characteristics of chromosomes of Korean indigenous waxy corn stocks.

Giemsa C-banding방법으로 한국 재래종 찰옥수수의 핵형 및 염색체 상에 존재하는 knob의 수와 위치 등을 확인하고 모식도를 통하여 염색체의 특성을 나타내고자 재래종 찰옥수수 5개의 자식 계통 (inbred stock)을 조사하였다. knob의 수는 6-12개이었고 평균 9.2개이었으며 계통별로 차이가 있었으며, knob을 가지고 있는 염색체도계통별로 차이가 있었다. YS-1의 경우 6번, 7번, 8번 그리고 9번 염색체에서 knob을 가지고 있었는데, MY-1의경우 1번, 2번, 3번, 6번, 「번 그리고 9번 염색체에서 knob을 가지고 있었다. 염색체의 장완과 단완의 비율, 염색체의 상대적 길이 등을 비교해 보기 위하여 YS-1과 MY-1 두 계통을 조사해 본 결과 두 계통 간에 차이가 있었고, 염색체의 상대적 길이는 YS-1이 큰 것으로 나타났다. Giemsa C-banding법은 재래종 찰옥수수의 계통별 특성과 이를 이용한 계통 분류에도 효과적으로 이용할 수 있을 것으로 생각된다.

Keywords

References

  1. Aguiar-Perecin, de, M. R. L. and C. G. Vosa. 1985. C-banding in maize; II. Identification of somatic chromosomes. Heredity 54, 37-42 https://doi.org/10.1038/hdy.1985.6
  2. Bennet, M. D., J. P. Gustafson and B. Smith. 1977. Variation in nuclear DNA in the genus Secale. Chromosoma 61, 149-176 https://doi.org/10.1007/BF00327398
  3. Bianch, A., M. V. Chatnekar and A. Ghidoni. 1963. Knobs in Italian maize. Chromosoma 14, 601-617 https://doi.org/10.1007/BF00326514
  4. Brown, W. L. 1949. Numbers and distribution of chromosome knobs in the United States maize. Genetics 34, 524-536
  5. Chen, C. C. 1969. The somatic chromosomes of maize. Can. J. Genet. 11, 752-754 https://doi.org/10.1139/g69-088
  6. Filion, W. G. and D. B. Walden. 1973. Karyotype analysis : The detection of chromosomal alternations in the somatic karyotypes of Zea mays L. Chromosoma 41, 183-194 https://doi.org/10.1007/BF00319695
  7. Gendel, S. M. and D. E. Fosket. 1979. The role of chromosomal proteins in the C-banding of Allium cepa chromosomes. Cytobios 22, 155-168
  8. Hadlaczky, G. Y. and L. Kalman L. 1975. Discrimination of homologous chromosomes of maize with Giemsa staining. Heredity 35, 317-374 https://doi.org/10.1038/hdy.1975.103
  9. Horn, J. D. and D. B. Walden. 1971. Fluorescent staining of euchromatin and heterochromatin in maize (Zea mays). Can. J. Genet. Cytol. 13, 811-815 https://doi.org/10.1139/g71-115
  10. Hsu, T. C. 1975. A possible function of constitutive heterochromatin : the body guard hypothesis. Genetics 79 (suppl), 137-150
  11. Lee, I. S., B. H. Choe and J. P. Gustafson. 1996. C-banding pattern of mitotic chromosome in Korean indigenous maize. Korean J. Crop Sci. 41 (4), 429-433
  12. Lee, I. S. and H. B. Lee. 1997. Heterochromatic knob number and karyotype in Korean indigenous maize. Korean J. Crop Sci. 42 (4), 446-451
  13. Mastenbroek, I., C. C. Cohen and J. M. J. de Wet. 1981. Seed protein and seedling isozyme patern of Zea mays and its closest relatives. Biochem. Syst. Ecol. 9, 179-183 https://doi.org/10.1016/0305-1978(81)90038-7
  14. Reeves, R. G. 1944. Chromosome knobs in relation to the origin of maize. Genetics 29, 141-147
  15. Rhoades, M. M. 1978. Genetic effects of heterochromatin in maize. In Maize breeding genetics. Edited by Balden D. B. Wiley. New York. pp. 641-671
  16. Sachan, J. K. S., R. Tanaka. 1977. Variation in pattern of C-banding in Zea chromosomes. Nucleus 20, 61-62
  17. Smith, J. S. C., M. M. Goodman and R. N. Lester. 1981. Variation within teosinte. I. Numerical analysis of morphological data. Econ. Bot. 35, 187-203 https://doi.org/10.1007/BF02858686
  18. Vosa, C. G. and M. L. R. de Aguiar-Perecin. 1972. New techniques for knob detection in mitotic chromosomes in maize and teosinte. Maize Genet. Coop. Newsl. 46, 165-167
  19. Ward, E. J. 1980. Banding patterns in maize mitotic chromosomes. Can. J. Genet. 22, 61-67 https://doi.org/10.1139/g80-009