Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Variation of Saponin Content in the Decoctions of Platycodi Radix

길경(桔梗)의 추출조건에 따른 사포닌 함량의 변화

  • Choi, Jae-Seok (Korea Research Institute of Chemical Technology) ;
  • Yoo, Dae-Seok (Korea Research Institute of Chemical Technology) ;
  • Choi, Yeon-Hee (Korea Research Institute of Chemical Technology) ;
  • Yon, Gyu-Hwan (Korea Research Institute of Chemical Technology) ;
  • Hong, Kyung-Sik (Korea Research Institute of Chemical Technology) ;
  • Lee, Byung-Hoe (Korea Research Institute of Chemical Technology) ;
  • Kim, Hye-Jin (Korea Research Institute of Chemical Technology) ;
  • Kim, Hyun-Ki (Korea Research Institute of Chemical Technology) ;
  • Kim, Eun-Ju (JangSaeng Doraji Research Institute of Biotechnology, JangSaeng Doraji Co. LTD.) ;
  • Roh, Seong-Hwan (JangSaeng Doraji Research Institute of Biotechnology, JangSaeng Doraji Co. LTD.) ;
  • Jeong, Young-Chul (Division of Food science, Jinju International University) ;
  • Kim, Young-Sup (Korea Research Institute of Chemical Technology) ;
  • Ryu, Shi-Yong (Korea Research Institute of Chemical Technology)
  • Published : 2007.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

We investigated the contents of crude saponin in the decoctions of Platycodi Radix prepared by the different extraction processes. Each lyophilized decoctions prepared by the different protocol were suspended in water and partitioned with ethylacetate (EtOAc) and n-butanol (BuOH), which gave the EtOAc fraction, BuOH fraction and the remaining water fraction, respectively. The content of crude saponin, which was estimated as the amount of BuOH fraction, and the HPLC profiles of the BuOH fraction were analyzed, and compared each others. The crude saponin content in the decoctions were increased proportionally to the increment of the extraction time, amount of water, extraction temperature and the number of repetition for extraction. Consequently, the optimized conditions were deduced to be suitable and recommendable for the preparation of Platycodi Radix ; the water amount needed for the extraction be 15-20 volumes to crude material, temperature for extraction be optimal at $85{\sim}100^{\circ}C$, extraction time be more than 5 hours and the repetition of extraction was 2 times.

Keywords

References

  1. 홍문화(1972) 한방처방의 통계적 연구(I), 생약학회지 3(2): 57-64
  2. Lee, S. H. and Lee, Y. C. (1991) Method of cultivating the perennial balloon flower, Patent No. 100045791, Korea
  3. Oka, M., Ota, N., Mino, Y., Iwashita, T. and Komura, H.(1992) Studies on the conformational aspects of inulin oligomers. Chem. Pharm. Bull. 40(5): 1203-1207 https://doi.org/10.1248/cpb.40.1203
  4. Ishii, H., Tori, K., Tozyo, T. and Yoshimura, Y. (1981) Saponins from roots of Platycodon grandiflorum. Part 1. Structure of prosaponins. J.C.S. Perkin 1: 1928-1933
  5. Ishii, H., Tori, K., Tozyo, T. and Yoshimura, Y. (1984) Saponins from roots of Platycodon grandiflorum. Part 2. Isolation and structure of new triterpene glycosides. J.C.S. Perkin 1: 661-668
  6. Saeki, T., Koike, K. and Nikaido, T.(1999) A Comparative study on commercial, botanical gardens and wild samples of the roots of Platycodon grandiflorum. Planta Med. 65: 428-431 https://doi.org/10.1055/s-1999-14021
  7. Nikaido, T., Koike, K., Mitsunagi, K. M. and Saeki, T.(1999) Two new triterpenoid saponins from Platycodon grandiflorum.. Chem. Pharm. Bull. 47(6): 903-904 https://doi.org/10.1248/cpb.47.903
  8. Kim, Y.P., Lee, E. B., Kim, S. Y., Li, D., Ban, H. S., Lim, S. S., Shin, K. H. and Ohuchi, K. (2001) Inhibition of prostaglandin E2 production by platycodin D isolated from the root of Platycodon grandiflorum.. Planta Med. 67(4): 362-364 https://doi.org/10.1055/s-2001-14317
  9. Shin, C. Y., Lee, W. J., Lee, E. B., Choi, E. Y. and Ko, K. H. (2002) Platycodin D and $D_3$ increase airway mucin release in vivo and in vitro in rats and hamsters. Planta Med. 68(3): 221-225 https://doi.org/10.1055/s-2002-23130
  10. Lee, K.J. and Jeong, H.G. (2002) Protective effect of Platycodi Radix on carbon tetrachloride-induced hepatotoxicity. Food Chem. Toxicol., 40: 517-525 https://doi.org/10.1016/S0278-6915(01)00104-1
  11. Lee, K.J., You, H.J., Park, S.J., Kim, Y.S., Chung, Y.C., Jeong, T.C. and Jeong, H.G.(2001) Hepatoprotective effects of Platycodon grandiflorum on acetaminophen-induced liver damage in mice. Cancer Lett., 174: 73-81 https://doi.org/10.1016/S0304-3835(01)00678-4
  12. Choi, C.Y., Kim, J.Y., Kim, Y.S., Chung, Y.C., Seo, J.K. and Jeong, H.G. (2001) Aqueous extract isolated from Platycodon grandiflorum elicts release of nitric oxide and tumor necrosis factor-${\alpha}$ from murine macrophages. International Immunopharmacology, 1: 1141-1151 https://doi.org/10.1016/S1567-5769(01)00047-9
  13. Choi, C.Y., Kim, J.Y., Kim, Y.S., Chung, Y.C., Hahm, K.S. and Jeong, H.G. (2001) Agumentation of macrophage functions by an aqueous extract isolated from Platycodon grandiflorum. Cancer Lett., 166: 17-25 https://doi.org/10.1016/S0304-3835(01)00440-2
  14. Kim, Y.S., Kim, J.S., Choi, S.U., Kim, J.S., Lee, H.S., Roh, S.H., Jeong, Y.C., Kim, Y.K. and Ryu, S.Y.(2005) Isolation of a new saponin and cytotoxic effect of saponins from the root of Platycodon grandiflorum on human tumor cell lines. Planta med. 71: 566-568 https://doi.org/10.1055/s-2005-864161