YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Molecular Analysis of Spontaneous Mutations in erm(A) and erm(C) Selected In vitro as a Constitutive MLS$_B$ Resistant Staphylococci

MLS$_B$계 항생물질 유도 내성 세균에서 In vitro로 선발된 지속성 내성형 erm(A)와 erm(C)의 분자적 특성 규명

  • Yoon, Eun-Jeong (College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University) ;
  • Jin, Sung-Hye (Department of Life Science, University of Seoul) ;
  • Choi, Eung-Chil (College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University) ;
  • Shim, Mi-Ja (Department of Life Science, University of Seoul)
  • 윤은정 (서울대학교 약학대학 종합 약학 연구소) ;
  • 진성혜 (서울시립대학교 생명과학과) ;
  • 최응칠 (서울대학교 약학대학 종합 약학 연구소) ;
  • 심미자 (서울시립대학교 생명과학과)
  • Published : 2007.04.30
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Abstract

The predominant Macrolides-Lincosamide-Streptogramin B (MLS$_B$) antibiotics resistance genes in staphylococci are erm(A) and erm(C). There is the phenomenon that the ratio of constitutively MLS$_B$ antibiotics resistance (cMLS) in erm(A) is much higher than in erm(C). Thus, we confirmed that the difference of the mutation ratio between erm(A) and erm(C) makes the phenomenon. We examined 8 staphylococci carrying inducibly expressed (iMLS) erm(A) or erm(C) genes. After overnight incubation in the presence of the non-inducer MLS$_B$ antibiotics, spontaneous mutants constitutively expressed MLS$_B$ resistance were selected. Against our expectation, the mutation ratio of erm(A) was lower than erm(C). Therefore, possibilities of other factors determining the ratio of cMLS phenotype might be concerned. All the mutants showed sequence alterations in translational attenuator and all the alterations seemed to give rise to change the second structure of mRNA to express constitutively. For erm(A), 4 different types of sequence deletions ranging from 72 bp to 122 bp and 3 different types of duplications ranging 24 bp to 93 bp were detected. Also, there were 9 different types of duplications ranging 15bp to 154bp in erm(C).

Keywords

References

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