Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Identification of Novel Mitochondrial Membrane Protein (Cdf 3) from Arabidopsis thaliana and its Functional Analysis in a Yeast System

  • Kim, Kyung-Min (Department of Environmental Horticulture, Sangju National University) ;
  • Jun, Do-Youn (Institute of Genetic Engineering, Kyungpook National University) ;
  • Kim, Sang-Kook (Institute of Genetic Engineering, Kyungpook National University) ;
  • Kim, Chang-Kil (Department of Environmental Horticulture, Sangju National University) ;
  • Kim, Byung-Oh (Department of Applied Biology, Sangju National University) ;
  • Kim, Young-Ho (Institute of Genetic Engineering, Kyungpook National University) ;
  • Park, Wan (Institute of Genetic Engineering, Kyungpook National University) ;
  • Sohn, Jae-Keun (Department of Agronomy, College of Agriculture, Kyungpook National University) ;
  • Hirata, Aiko (Department of Integrate Biosciences, Graduate School of Frontier Sciences, The University of Tokyo) ;
  • Kawai-Yamada, Maki (Institute of Molecular & Cellular Biosciences, University of Tokyo) ;
  • Uchimiya, Hirofumi (Institute of Molecular & Cellular Biosciences, University of Tokyo) ;
  • Kim, Dai-Hee (Department of Environmental Horticulture, Sangju National University) ;
  • Sul, Ill-Whan (Department of Biotechnology, Daegu University of Foreign Studies)
  • Published : 2007.06.30
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Abstract

We screened the Arabidopsis cDNA library to identify functional suppressors of AtBI-1, a gene that suppresses cell death induced by Bax gene expression in yeast. Cdf 3 encodes a 118-amino-acid protein with a molecular mass of 25 kDa. This protein has two uncharacterized domains at amino acids residues 5-64 and 74-117. In the present study, CDF3 was found to induce growth defects in yeast and arrested yeast growth, although the cell-growth defect was somewhat less than that of Bax. Its localization in the inner mitochondria was essential for suppression of yeast-cell proliferation. The morphological abnormality of the intracellular network, which is a hallmark of AtBI-1, was attenuated by Cdf3 expression.

Keywords

References

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