Initial tissue response of biodegradable membrane in rat subcutaneous model

백서 피하층에서 흡수성 차단막의 초기 조직 반응

  • Lim, Hyun-Chang (Department of Periodontology, College of Dentistry, Yonsei University) ;
  • Chae, Gyung-Joon (Department of Periodontology, College of Dentistry, Yonsei University) ;
  • Jung, Ui-Won (Department of Periodontology, College of Dentistry, Yonsei University) ;
  • Kim, Chang-Sung (Department of Periodontology, College of Dentistry, Yonsei University) ;
  • Lee, Yong-Keun (Department and Research Institute of Dental Biomaterial and Bioengineering, College of Dentistry, Yonsei University) ;
  • Cho, Kyoo-Sung (Department of Periodontology, College of Dentistry, Yonsei University) ;
  • Kim, Chong-Kwan (Department of Periodontology, College of Dentistry, Yonsei University) ;
  • Choi, Seong-Ho (Department of Periodontology, College of Dentistry, Yonsei University)
  • 임현창 (연세대학교 치과대학 치주과학교실) ;
  • 채경준 (연세대학교 치과대학 치주과학교실) ;
  • 정의원 (연세대학교 치과대학 치주과학교실) ;
  • 김창성 (연세대학교 치과대학 치주과학교실) ;
  • 이용근 (연세대학교 치과대학 치과생체재료학교실) ;
  • 조규성 (연세대학교 치과대학 치주과학교실) ;
  • 김종관 (연세대학교 치과대학 치주과학교실) ;
  • 최성호 (연세대학교 치과대학 치주과학교실)
  • Published : 2007.12.31

Abstract

Purpose: Various kinds of biodegradable membranes are currently used in dental clinics. And the frequency and the necessity of their usage are increasing due to their numerous advantages. Therefore it is important to understand the difference of various membranes and histological reaction against implanted membranes. Materials and Methods: Biodegradable membranes of $Biogide^{(R)}$, $Resolute^{(R)}$, and $Tutodent^{(R)}$ were cut into small pieces by $1.0{\times}0.5cm$. The membranes were implanted 1.5cm apart from each other under the epithelium on the skull of 18 Sprague Dawley rats. The animals were sacrificed at 3, 7, and 14 days after surgical procedure. The specimens were examined by histological analysis. Results: 1. Early period after implantation of the membranes showed connective tissues surrounding membranes and there were a few inflammatory cells present. 2. In $Biogide^{(R)}$ and $Tutodent^{(R)}$ specimens, inflammatory cells and surrounding tissues were shown to infiltrate from outside with slight density difference inside. In $Resolute^{(R)}$ specimens, membranes were fragmented. Inflammatory cells and connective tissues were also observed inside. 3. In $Resolute^{(R)}$ specimen, giant cells were present which implicates that foreign body reaction has occurred. 4. $Biogide^{(R)}$ had lower integrity than other membranes and is not enough to be used alone in defect area. However, $Resolute^{(R)}$ had superior firmness than others. $Tutodent^{(R)}$ had middle level of integrity. Conclusion: This experimental model enabled to observe early inflammatory reactions and morphological changes of materials and can be used to develop and evaluate the efficacy of biodegradable membranes. Duplication of standardized human oral environment will be required in future experiments.

Keywords

References

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