Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Bone regenerative effects of recombinant human bone morphogenetic protein-2 employed protein transduction domain

Protein transduction domain을 이용한 recombinant human bone morphogenetic protein-2의 골재생효과

  • Jung, Sung-Won (Department of periodontology, Research institute for periodontal Regeneration, College of Dentistry, Yonsei University) ;
  • Kim, Nam-Hee (Department of Oral pathology, Oral Cancer Research institute, College of Dentistry, Yonsei University) ;
  • Yook, Jong-In (Department of Oral pathology, Oral Cancer Research institute, College of Dentistry, Yonsei University) ;
  • Kim, Chang-Sung (Department of periodontology, Research institute for periodontal Regeneration, College of Dentistry, Yonsei University) ;
  • Kim, Hyung-Jun (Department of Oral & Maxillofacial surgery, College of Dentistry, Yonsei University) ;
  • Cho, Kyoo-Sung (Department of periodontology, Research institute for periodontal Regeneration, College of Dentistry, Yonsei University)
  • 정성원 (연세대학교 치주과학교실, 치주조직재생연구소) ;
  • 김남희 (연세대학교 구강병리학교실, 구강조양연구소) ;
  • 육종인 (연세대학교 구강병리학교실, 구강조양연구소) ;
  • 김창성 (연세대학교 치주과학교실, 치주조직재생연구소) ;
  • 김형준 (연세대학교 구강악안면외과학교실) ;
  • 조규성 (연세대학교 치주과학교실, 치주조직재생연구소)
  • Published : 2007.09.30
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Abstract

Bone morphogenetic proteins(BMPs) are regarded as members of the transforming growth $factor-{\beta}$ superfamily with characteristic features in their amino acid sequences. A number of studies have demonstrated the biologic activities of BMPs, which include the induction of cartilage and bone formation. Recently there was a attempt to overcome a limitation of mass production, and economical efficieny of rh-BMPs. The method producing PTD by using bacteria have advantages of acquiry a mass of proteins. Hences, a new treatment which deliver protein employed by protein transduction domain(PTD) has been tried. The purpose of this study was to evaluate the bone regenerative effect of TATBMP-2 and TAT-HA2-BMP-2 employed by PTD from HlV-1 TAT protein for protein translocation in the rat calvarial model. An 8mm calvarial, critical size osteotomy defect was created in each of 32 male Spraque-Dawley rats(weight $250{\sim}300g$). The animals were divided into 4 groups of 32 animals each (4 animals/group/healing interval). The defect was treated with TATBMP-2/ACS(Absorbable collagen sponge) (TATBMP-2 0.1mg/ml), TAT-HA2-BMP-2/ACS(TAT-HA2-BMP-2 0.1mg/ml), ACS alone or left untreated for surgical control(negative control). The rats were sacrificed at 2 or 8 weeks postsurgery, and the results were evaluated histologically. The results were as follows: New bone formation were not significantly greater in the TATBMP-2/ACS group relative to negative, and positive control groups. New bone was evident at the defect sites in TAT-HA2-BMP-2/ACS group relative to negative, positive control and TATBMP-2 groups. There were a little bone regeneration in TATBMP-2 groups. While, enhanced local bone formation were observed in TAT-HA2-BMP-2 group. But, The results was not the same in all rat defects. Therefore, further investigations are required to develop a method. which disperse homogenously, and adhere to target cells.

Keywords

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