Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Effect of deproteinized bovine bone mineral soaked in inorganic polyphosphate on bone regeneration

무기인산염과 탈단백우골의 혼합사용이 골재생에 미치는 효과

  • Na, Seong-Yoon (Department of Periodontology and Institute of Oral Biology, School of Dentistry, Kyung Hee University) ;
  • Kwon, Young-Hyuk (Department of Periodontology and Institute of Oral Biology, School of Dentistry, Kyung Hee University) ;
  • Park, Joon-Bong (Department of Periodontology and Institute of Oral Biology, School of Dentistry, Kyung Hee University) ;
  • Herr, Yeek (Department of Periodontology and Institute of Oral Biology, School of Dentistry, Kyung Hee University) ;
  • Chung, Jong-Hyuk (Department of Periodontology and Institute of Oral Biology, School of Dentistry, Kyung Hee University)
  • 나성윤 (경희대학교 치의학전문대학원 치주과학교실, 구강생물학연구소) ;
  • 권영혁 (경희대학교 치의학전문대학원 치주과학교실, 구강생물학연구소) ;
  • 박준봉 (경희대학교 치의학전문대학원 치주과학교실, 구강생물학연구소) ;
  • 허익 (경희대학교 치의학전문대학원 치주과학교실, 구강생물학연구소) ;
  • 정종혁 (경희대학교 치의학전문대학원 치주과학교실, 구강생물학연구소)
  • Published : 2007.03.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was performed to evaluate the effect of deproteinized bovine bone mineral soaked in inorganic polyphosphate on bone regeneration in the calvaria of rabbit in the procedure of guided bone regeneration with titanium reinforced expanded polytetrafluoroethylene(TR-ePTFE) membrane. The rabbits were divided into four groups. Control group used TR-ePTFE membrane filled with de-proteinized bovine bone mineral, experimental group I used TR-ePTFE membrane and deproteinized bovine bone mineral soaked in 4% inorganic polyphosphate, experimental group II and III used TR-ePTFE membrane and deproteinized bovine bone mineral soaked in 8% or 16% inorganic poly-phosphate respectively. After decortication in the calvaria, GBR procedure was performed on 8 rabbits with only TR-ePTFE membrane or titanium reinforced ePTFE membrane filled with deproteinized bovine bone mineral soaked in inorganic polyphosphate. The animals were sacrificed at 4 weeks, and 8 weeks af-ter the surgery. Non-decalcified specimens were processed for histologic analysis, and new bone for-mation was assessed by histomorphometric as well as statical analysis. 1. Both control group and experirrental group dermnstrated increasing of new bone formation until 8weeks. 2. At 8 weeks, experimental group I and group II showed the significant difference compared to control group in new bone formation. Especially experimental group II showed the most in-creasing of new bone formation. 3. The higher concentration of inorganic polyphosphate filled, the more volume of bone formation pro-moted, but experimental group III did not reveal significant difference compared to contol group. 4. Deproteinized bovine bone mineral did not resorbed at all until 8 weeks. These results suggest that inorganic polyphosphate has a promoting effect on bone regeneration. possibly by enhancing osteoconductivity of the carrier and by increasing osteoinductivity of the defected alveolar bone tissue, but not as we respect.

Keywords

References

  1. Reddi AH, Weintroub S, Muthukumaran N. Biologic principles of bone induction. Orthop Clin North Am 1987;18:207-212
  2. Burchardt H. The biology of bone graft repair. Clin Orthop Rel Res 1983;174:28-42
  3. Dahlin C, Linde A, Gottlow J, Nyman S. Healing of bone defects by guided tissue regeneration. Plastic Reconst Sur 1988;81: 672-677 https://doi.org/10.1097/00006534-198805000-00004
  4. Kostopoulos L, Karring T. Augmentation of the rat mandible using guided tissue regeneration. Clin Oral Impl Res 1994;5:75-82 https://doi.org/10.1034/j.1600-0501.1994.050203.x
  5. Urist MR. Bone Formation by autoinduction. Science 1965:150:893-899 https://doi.org/10.1126/science.150.3698.893
  6. Linkhart TA, Mohan S, Baylink DJ. Growth factors for bone growth and repair: IGF, TGF-$\beta$ and BMF. Bone 1996;19:1-12 https://doi.org/10.1016/8756-3282(96)00116-0
  7. Bostrom MFG, Asnis P. Transforming growth factor beta in fracture repair. Clin Orthop Rel Res 1998;355:124-131
  8. Ilizarov GA, The tension-stress effect on the genesis and growth of tissues: Part I. The influence of stability of fixation and soft tissue preservation. Clin Orthop 1989;238:249-281
  9. Ilizarov GA, The tension-stress effect on the genesis and growth of tissues: Part II. The influence of rate and frequency of distraction. Clin Orthop 1989;239:263-285
  10. Linkhart TA, Mohan S, Baylink DJ. Growth factors for bone growth and repair: IGF, TGF-$\beta$ and BMF. Bone 1996:19:1-12 https://doi.org/10.1016/8756-3282(96)00116-0
  11. Bostrom MPG, Asnis P, Transforming growth factor beta in fracture repair. Clin Orthop Rel Res 1998; 355:124-131
  12. Nyman S, Lindhe J, Karring T. New attachment in the human periodontium by guided tissue regeneration. J Clin Periodontol 1982;9:290-296 https://doi.org/10.1111/j.1600-051X.1982.tb02095.x
  13. Dahlin C, Linde A, Gottlow J, Nyman S. Healing of bone defects by guided tissue regeneration. Plastic Reconst Sur 1988;81:672-677 https://doi.org/10.1097/00006534-198805000-00004
  14. Sandberg E, Dahlin C, Linde A. Bone regeneration by the osteopromotive technique using bioabsorbable membranes. An experimental study in rats. Int J Oral Maxillofac Surg 1993;51:1106-1114 https://doi.org/10.1016/S0278-2391(10)80450-1
  15. Becker W, Becker B, Handelsman M, Ochsenbein C, Albrektsson T. Guided tissue regeneration for implant placed into extraction sockets: A study in dogs. J Periodontol 1991;62:703-709 https://doi.org/10.1902/jop.1991.62.11.703
  16. Bragger D, Hammerle CHF, Lang NP. Immediate transmucosal implants using the principle of guided tissue regeneration: A cross-sectional study comparing the clinical outcome 1 year after immediate to standard implant placement. Clin Oral Impl Res 1997;7: 268-276 https://doi.org/10.1034/j.1600-0501.1996.070309.x
  17. Buser D, Bragger D, Land NP, Nyman S. Regeneration and enlargement of jaw bone using guided tissue regeneration. Clin Oral Impl Res 1990;1:22-32 https://doi.org/10.1034/j.1600-0501.1990.010104.x
  18. Dahlin C, Lekholm U, Becker W, et al, Treatment of fenestration and dehiscence bone defects around oral implants using the guided tissue regeneration technique: A prospective multicenter study. Int J Oral Maxillofac Imp 1995; 10: 312-318
  19. Hammerle CHF, Olah AJ, Schmid J, et al. The biological effect of deproteinized bovine bone on bone neoformation on the rabbit skull. Clin Oral Impl Res 1997;8:198-207 https://doi.org/10.1034/j.1600-0501.1997.080306.x
  20. Lang NP, Hammerle CHF, Bragger D, Lehmann B, Nyman SR, Guided tissue regeneration in jaw bone defects prior to implant placement, Clin Oral Impl Res 1994;5:92-97 https://doi.org/10.1034/j.1600-0501.1994.050205.x
  21. Donos N, Kostopoulos L, Karring T. Augmentation of the rat jaw with autogenic corti co-cancellous bone grafts and guided tissue regeneration. Clin Oral Impl Res 2002; 13:192-202 https://doi.org/10.1034/j.1600-0501.2002.130210.x
  22. Donos N, Kostopoulos L, Karring T. Augmentation of the mandible with Gill and onlay cortical bone grafting. An experimental study in the rat. Clin Oral Impl Res 2002; 13:175-184 https://doi.org/10.1034/j.1600-0501.2002.130208.x
  23. Von Arx T, Cochran DL, Hermann JS, Schenk RK, Buser D. Lateral ridge augmentation using different bone fillers and barrier membrane application. A histologic and histomorphometric pilot study in the mandible. Clin Oral Impl Res 2001;12:260-269 https://doi.org/10.1034/j.1600-0501.2001.012003260.x
  24. Nevins M, Mellonig JT. Enhancement of the damaged edentulous ridge to receive dental implants: A combination of allograft and the GORE-TEX membrane. Int J Periodont Rest Dent 1992;12:97-111
  25. Becker W, Schenk R, Higuchi K, Lekholm U, Becker BE. Variations in bone regeneration adjacent to implants augmented with barrier membranes alone or with demineralized freeze -dried bone or autologous grafts: A study in dogs. Int J Oral Maxillofac Imp 1995;10: 143-154
  26. Jovanovic SA, Nevins M. Bone formation utilizing titanium-reinforced barrier membranes, Int J Periodont Rest Dent 1995;15:57-69
  27. Jovanovic SA, Schenk RK, Orsini M, Kenney Eb. Supracrestal bone formation around dental implants: An experimental dog study. Int J Oral .Maxillofac Implants 1995;10:23-31
  28. Jovanovic SA, Nevins M. Bone formation utilizing titanium-reinforced barrier membranes. Int J Periodont Rest Dent 1995;15:57-69
  29. Leckholm U, Becker W, Dahlin C, et al, The role of early versus late removal of Gemembrane on bone formation at oral implants placed into immediate extraction sockets. An experimental study in dogs. Clin Oral Impl Res 1993;4:121-129 https://doi.org/10.1034/j.1600-0501.1993.040302.x
  30. Simon M, Trisi P, Piattellli A. Vertical ridge augmentation using a membrane technique associated with osseointegrated implants. Int J Periodont Rest Dent 1994;14:496-511
  31. Jovanovic SA, Spiekermann H, Richter EJ. Bone regeneration around titanium implants in dehiscenced defect sites: A clinical study. Int J Oral Maxillofac Implants 1992;7: 233-245
  32. Donos N, Kostopoulos L, Karring T. Augmentation of the rat jaw with autogenic cortico-cancellous bone grafts and guided tissue regeneration. Clin Oral Impl Res 2002; 13:192-202 https://doi.org/10.1034/j.1600-0501.2002.130210.x
  33. Donos N, Kostopoulos L, Karring T. Augmentation of the mandible with GTR and onlay cortical bone grafting. An experimental study in the rat. Clin Oral Impl Res 2002;13:175-184 https://doi.org/10.1034/j.1600-0501.2002.130208.x
  34. Von Arx T, Cochran DL, Hermann JS, Schenk RK, Buser D. Lateral ridge augmentation using different bone fillers and barrier membrane application. A histologic and histomorphometric pilot study in the mandible. Clin Oral Impl Res 2001;12:260-269 https://doi.org/10.1034/j.1600-0501.2001.012003260.x
  35. Nevins M, Mellonig JT. Enhancement of the damaged edentulous ridge to receive dental implants: A combination of allograft and the GORE-TEX membrane. Int J Periodont Rest Dent 1992;12:97-111
  36. Donos N, Kostopoulos L, Karring T. Alveolar ridge augmentation by combining autogenous mandibular bone grafts and non-resorbable membranes. An experimental study in the rat. Clin Oral Impl Res 2002;13:185-191 https://doi.org/10.1034/j.1600-0501.2002.130209.x
  37. Malchiodi L, Scarano A, Quaranta M, Piattelli A. Rigid fixation by means of titanium mesh in edentulous ridge expansion for horizontal ridge augmentation in the maxilla. Int J Oral Maxillofac Implants 1998;13:701-705
  38. Slott C, Lundgren D. Augmentation of calvarial tissue using non-permeable silicone domes and bovine bone mineral. An experimental study in the rat. Clin Oral ImplRes 1999;10:468-476 https://doi.org/10.1034/j.1600-0501.1999.100605.x
  39. Piattelli A, Scarano M, Corigliano Piattelli M, Comparison of bone regeneration with the use of mineralized and demineralized freeze-dried bone allografts: A histologic and histochemical study in man, Biomaterials 1996;17:1127 -1131 https://doi.org/10.1016/0142-9612(96)85915-1
  40. Nevins M, Mellonig JT, Clem DSC III, Reiser GM, Buser DA, Implants in regenerated bone: long-term survival, Int J Periodont Rest Dent 1998;18:35-45