Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antioxidant Activity of Heated Licorice (Glycyrrhiza uralensis Fisch) Extracts in Korea

열처리한 국산 감초추출물의 항산화활성

  • Woo, Koan-Sik (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Hwang, In-Guk (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Noh, Young-Hee (Dept. of Beauty Industry, Konyang University) ;
  • Jeong, Heon-Sang (Dept. of Food Science and Technology, Chungbuk National University)
  • Published : 2007.06.30
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Abstract

Antioxidative activity and polyphenol contents of heated licorice in Korea extracted by ethyl.acetate (EtOAc) and ethyl.alcohol (EtOH) were evaluated at various heating temperatures (110, 120, 130, 140, and $150^{\circ}C$), times (1, 2, 3, 4, and 5 hr), and moisture contents (10, 20, 30, 40, and 50%). Maximum extraction yields of EtOAc extract was 10.9% at $130^{\circ}C$, 3 hr, and 50% moisture content and that of EtOH extract was 25.0% at $120^{\circ}C$, 2 hr, and 20% moisture content, whereas those of control were 0.8 and 15.8%, respectively. The highest total polyphenol content was 845.67 mg/100 g in EtOH extract at $120^{\circ}C$, 2 hr, and 20% moisture content (control: 277.00 mg/100 g). The antioxidative activity ($IC_{50}$) was the highest value of 0.53 mg/mL in EtOAc extract at $120^{\circ}C$, 2 hr, and 20% moisture content (control: 12.34 mg/mL). The highest ascorbic acid equivalent antioxidant activity value of 1,584 mg ascorbic acid (AA) eq was obtained from EtOAc extract at $120^{\circ}C$, 2hr, and 40% moisture content (control: 1,263 mg AA eq). Optimum heating conditions for the improvement of antioxidative activity of licorice in Korea was $120^{\circ}C$, 2 hr, and $20{\sim}40%$ moisture content.

국산 감초를 처리온도, 시간 및 첨가수분함량을 변수로하여 열처리한 다음 에틸아세테이트와 에탄올로 추출하여 성분 및 생리활성 변화를 분석하고 반응표면분석으로 최적화 조건을 조사하였다. 에틸아세테이트 추출물의 추출수율은 $130^{\circ}C$, 3시간, 가수량 50%일 때 10.90%로 무처리구의 0.80%보다 높았으며, 에탄올 추출물은 $120^{\circ}C$, 2시간, 가수량 20%로 처리한 시료가 25.00%로 무처리구의 15.80%보다 높았다. 총 폴리페놀 함량은 열처리구가 무처리구보다 높았으며, 에탄올 추출물의 $120^{\circ}C$, 2시간, 가수량 20% 처리구가 845.67 mg/100 g으로 무처리구의 277.00 mg/100 g보다 높았다. 항산화활성($IC_{50}$)은 열처리구가 무처리구보다 높게 나타났으며, 에틸아세테이트 추출물 $120^{\circ}C$, 2시간, 가수량 20% 처리구에서 0.53 g/L로 무처리구의 12.34 g/L보다 매우 높게나타났다. 총항산화력(AEAC)은 모든 처리구에서 무처리구보다 높게 나타났는데 에틸아세테이트 추출물 $120^{\circ}C$, 2시간, 가수량 40% 처리구에서 1,584 mg AA eq로 무처리구의 1,263 mg AA eq보다 높게 나타났다. 본 연구결과 감초의 항산화활성, 총 폴리페놀 함량 등을 증가시키기 위한 최적의 열처리 조건은 $120^{\circ}C$, 2시간, 가수량 $20{\sim}40%$로 판단되었다.

Keywords

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