Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Growth and Phosphate Uptake of the Toxic Dinoflagellate Gymnodinium catenatum Isolated from Yeosuhae Bay, South Korea

여수해만산 유독 와편모조류 Gymnodinium catenotum (Graham)의 용존태 무기인에 대한 성장 및 흡수

  • Oh, Seok-Jin (Korea Inter-University Institute of Ocean Science, Pukyong National University) ;
  • Yoon, Yang-Ho (Faculty of Marine Technology, Chonnam National University) ;
  • Yang, Han-Soeb (Korea Inter-University Institute of Ocean Science, Pukyong National University)
  • 오석진 (부경대학교 해양과학공동연구소) ;
  • 윤양호 (전남대학교 해양기술학부) ;
  • 양한섭 (부경대학교 해양과학공동연구소)
  • Published : 2007.04.30
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Abstract

We investigated the growth and phosphate uptake of a toxic dinoflagellate, Gymnodinium catenatum, isolated from Yeosuhae Bay, South Korea. A short-term phosphate uptake experiment revealed that its maximum uptake and the half-saturation constant were 1.39 pmol/cell/hr and $2.65{\mu}M$, respectively. In a semicontinuous culture, the maximum specific growth rate and minimum phosphorus cell quota of G. catenatum were 0.39/day and 1.27 pmol/cell, respectively. Thus, G. catenatum is a poor competitor in terms of inorganic nutrient use and is unlikely to form blooms in Yeosuhae Bay.

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References

  1. Abe, H. 1985. Ouyo Sugaku Nyumon. Baifu Kan, Tokyo, 1-215
  2. Baba, T., S. Hiyama and T. Tainaka. 2001. Vertical migration of the toxic dinoflagellate Gymnodinium catenatum and toxicity of cultured oyster in Senzaki Bay, Yamaguchi Prefecture. Bull. Plankton Soc. Jap., 48, 95-99
  3. Blackburn, S.I, G.M. Hallegraeff and C.J. Bolch. 1989. Vegetative reproduction and sexual life cycle of the toxic dinoflagellate Gymnodinium catenatum from Tasmania. Austral. J. Phycol., 25, 577-590 https://doi.org/10.1111/j.1529-8817.1989.tb00264.x
  4. Carrada, G.C., R. Casotti, M. Modigh and V. Saggiomo. 1991. Presence of Gymnodinium catenatum (Dinophyceae) in a coastal Mediterranean lagoon. J. Plankton Res., 13, 229-238 https://doi.org/10.1093/plankt/13.1.229
  5. Doblin, M.A., S.I. Blackburn and G.M. Hallegreff. 1999. Growth and biomass stimulation of the toxic dinoflagellate Gymnodinium catenatum (Graham) by dissolved organic substances. J. Exp. BioI. Ecol., 236, 33-47 https://doi.org/10.1016/S0022-0981(98)00193-2
  6. Doblin, M.A., P.A. Thompson, A.T. Revill, E.C.V. Butler, S.I. Blackburn and G.M. Hallegraeff. 2006. Vertical migration of the toxic dinoflagellate Gymnodinium catenatum under different concentration of nutrients and humic substances in culture. Harm. Algae, 5, 665-677 https://doi.org/10.1016/j.hal.2006.02.002
  7. Droop, M.R. 1973. Some thoughts on nutrient limitation in algae. J. Phycol., 9, 264-272
  8. Dugdale, R.C. 1967. Nutrient limitation in the sea: dynamic, identification, and significance. Limnol. Oceanogr., 12, 685-695 https://doi.org/10.4319/lo.1967.12.4.0685
  9. Eppley, R.W., J.N. Rogers and J.J. McCarthy. 1969. Half-saturation constants for uptake of nitrate and ammonium by marine phytoplankton. Limnol. Oceanogr., 14, 912-920 https://doi.org/10.4319/lo.1969.14.6.0912
  10. Estrada, M., F.J. Sanchez and S. Fraga. 1984. Gymnodinium catenatum (Graham) en las rias galle gas (No de Espana). Inv. Pesq., 48, 31-40
  11. Fermin, E.G., F.G. Figueiras, B. Arbones and M.L. Villarino. 1996. Short-time scale development of Gymnodinium catenatum population in the Ria de Vigo (NW Spain). J. Phycol., 32, 212-221 https://doi.org/10.1111/j.0022-3646.1996.00212.x
  12. Figueiras, F.G. and Y. Pazos. 1991. Hydrography and phytoplankton of the Ria de Vigo before and during a red tide of Gymnodinium catenatum Graham. J. Plankton Res., 13, 589-608 https://doi.org/10.1093/plankt/13.3.589
  13. Figueiras, F.G., K.J. Jones, A.M. Mosquera, X.A. Alvarez-Salgado, A. Edwards and M. MacDougall. 1994. Red tide assemblage formation in an estuaries upwelling ecosystem: Ria de Vigo. J. Plankton Res., 16, 857-878 https://doi.org/10.1093/plankt/16.7.857
  14. Flynn, K.J., K. Flynn, E.H. John, B. Reguera, M.l. Reyero and J.M. Franco. 1996. Changes in toxins, intracellular and dissolved free amino acids of the toxic dinoflagellate Gymnodinium catenatum in response to changes in inorganic nutrients and salinity. J. Plankton Res., 18, 2093-2111 https://doi.org/10.1093/plankt/18.11.2093
  15. Fraga, S., S.M. Gallager and D.M. Anderson. 1989. Chain-forming dinoflagellates: An adaptation to red tides. In: Red Tide: Biology, Environmental Science, and Toxicology. Okaichi, T., D.M. Anderson and T. Nemoto, eds. Elsevier, New York, 281-284
  16. Fraga, S. and A. Bakun, 1993. Global climate change and harmful algal blooms: the example of Gymnodinium catenatum on the Galician coast. In: Toxic Phytoplankton Blooms in the Sea. Smayda, T.J. and Y. Shimizu, eds. Elsevier, Amsterdam, 59-65
  17. Franca, S. and J.F. Almeida. 1989. Paralytic shellfish poisons in bivalve molluscs on the Portuguese coast caused by a bloom of the dinoflagellate Gymnodinium catenatum. In: Red Tides: Biology, Environmental Science, and Toxicology. Okaichi, T., D.M. Anderson and T. Nemoto, eds. Elsevier, New York, 93-96
  18. Graham, H.W. 1943. Gymnodinium catenatum, a new dinoflagellate from the Gulf of California. Trans. Am. Microsc. Soc., 62, 259-261 https://doi.org/10.2307/3223028
  19. Guillard, R.R.L. and D. Ryther. 1962. Studies of marine planktonic diatoms. l. Cyclotella nana Hustedt and Detonula confervacea (Cleve) Gran. Can. J. Microbiol., 8, 229-239 https://doi.org/10.1139/m62-029
  20. Guillard, R.R.L. and P.E. Hargraves. 1993. Strichochrysis immobilis is a diatom, not a chrysophyte. Phycologia, 32, 234-236 https://doi.org/10.2216/i0031-8884-32-3-234.1
  21. Guillard, R.R.L. 1995. Culture methods. In: Manual on Harmful Marine Microalage. Hallegraeff G.M., D.M. Anderson and A.D. Cembella, eds. UNESCO, Paris, 45-62
  22. Hada, Y. 1967. Protozoan plankton of the Inland Sea, Setonaikai. l. The mastigophora. Bull. Suzugamine Woman's Coll., Nat. Sci., 13, 1-26
  23. Hallegraeff, G.M. and C.E. Sumner. 1986. Toxic plankton blooms affect shellfish farms. Austral. Fish., 45, 15-18
  24. Hallegraeff, G.M. and C.J. Bolch. 1992. Transport of diatom and dinoflagellate resting spores in ships' ballast water: implications for plankton biogeography and aquaculture. J. Plankton Res., 14, 1067-1084 https://doi.org/10.1093/plankt/14.8.1067
  25. Hallegraeff, G.M. 1993. A review of harmful algal blooms and their apparent global increase. Phycologia, 32, 79-99 https://doi.org/10.2216/i0031-8884-32-2-79.1
  26. Harrison, P.J., J.S. Parslow and H.L. Conway. 1989. Determination of nutrient uptake kinetic parameters: a comparison of methods. Mar. Ecol. Prog. Ser., 52, 301-312 https://doi.org/10.3354/meps052301
  27. Ikeda, T., S. Matsuno, S. Sato, T. Ogata, M. Kodama, Y. Fukuyo and H. Takayama. 1989. First report on paralytic shellfish poisoning caused by Gymnodinium catenatum Graham (Dinophyceae) in Japan. In: Red Tides: Biology, Environmental Science and Toxicology. Okaichi, T., D.M. Anderson and T. Nemoto, eds. Elsevier, New York, 411-414
  28. Jeon, J.K. and M.S. Han. 1998. Monitoring of intoxication and toxin composition on wild mussels (Mytilus corsucus) from coastal waters near Koje Island, Korea in 1996 and 1997. J. Kor. Fish. Soc., 31, 817-822
  29. Keller, M.D., R.C. Selvin, W. Claus and R.R.L. Guillard. 1987. Media for the culture of oceanic ultraphytoplankton. J. Phycol., 23, 633-638 https://doi.org/10.1111/j.1529-8817.1987.tb04217.x
  30. Kim, H.G., K. Matsuoka, S.G. Lee and K.H. An. 1996. The occurrence of a dinoflagellate Gymnodinium catenatum from Chinhae Bay, Korea. J. Kor. Fish. Soc., 29, 837-842
  31. Kim, C.H. 1998. Development of PSP toxigenic dinoflagellates and toxin production in Korean coastal waters. In: Harmful algal blooms in Korea and China. Kim, H.G., S.G. Lee and C.K. Lee, eds. National Fisheries Research and Development Institute, Pusan, 1-20
  32. Lee, C.K., S.G. Lee and H.G. Kim. 1999. Occurrence and abundance of three morphologically similar dinoflagellates, Cochlodinium polykrikoides, Gyrodinium impudicum and Gymnodinium catenatum in the southern coast of Korea. Bull. Natl. Fish. Res. Dev. Int. Korea, 57, 131-139
  33. Lee, C.K., H.C. Kim, S.G. Lee, C.S. Jung, H.G. Kim and W.A. Lim. 2001. Abundance of harmful algae, Cochlodinium polykrikoides, Gyrodinium impudicum and Gymnodinium catenatum in the coastal area of South Sea of Korea and their effects of temperature, salinity and irradiance and nutrient on the growth in culture. J. Kor. Fish. Soc., 34, 536-544
  34. MacIsaac, J.J., G.S. Grunseich, H.E. Glover and C.M. Yentsch. 1979. Light and nutrient limitation in Gonyaulax excavata: nitrogen and carbon trace results. In: Toxic Dinoflagellate Blooms. Taylor, D.L. and H.H. Seliger, eds. Elsevier, New York, 107-110
  35. Matsuda, A., T. Nishijima and K. Fukami. 1999. Effects of nitrogenous and phosphorus nutrients on the growth of toxic dinoflagellate Alexandrium catenella. Nippon Suisan Gakkaishi, 65, 847-855 https://doi.org/10.2331/suisan.65.847
  36. Matsuoka, K. and Y. Fukuyo. 1994. Geographical distribution of the toxic dinoflagellate Gymnodinium catenatum Graham in Japanese coastal waters. Bot. Mar., 37, 495-503 https://doi.org/10.1515/botm.1994.37.6.495
  37. Margalef, R. 1978. Life-forms of phytoplankton as survival alternatives in an unstable environment. OceanoI. Acta, 1, 493-509
  38. Morel, F.M.M. 1987. Kinetics of nutrient uptake and growth in phytoplankton. J. PhycoI., 23, 137-150
  39. Morey-Gaines, G. 1982. Gymnodinium catenatum Graham (Dinophyceae): morphology and affinities with armoured forms. Phycologia, 21, 154-163 https://doi.org/10.2216/i0031-8884-21-2-154.1
  40. Munk, W.H. and G.A. Riley, 1952. Absorption of nutrients by aquatic plants. J. Mar. Res., 11, 215-240
  41. Nakamura, Y. 1985. Ammonium uptake kinetics and interactions between nitrate and ammonium uptake in Chattonella antiqua. J. Oceanogr. Soc. Jap., 41, 33-38 https://doi.org/10.1007/BF02109929
  42. Nishioka, J., Y Wada and Y Imanishi. 1993. On the occurrences of Gymnodinium catena tum (Dinophyceae) in Kumihama Bay. Bull. Kyoto. Inst. Ocean. Fish., 16, 43-49
  43. Oh, S.J., T. Yamamoto, Y. Kataoka, O. Matsuda, Y. Matsuyama and Y. Kotani. 2002. Utilization of dissolved organic phosphorus by the two toxic dinoflagellates, Alexandrium tamarense and Gymnodinium catenatum (Dinophyceae). Fish. Sci., 68, 416-424 https://doi.org/10.1046/j.1444-2906.2002.00440.x
  44. Oh, S.J. and Y.H. Yoon. 2004. Effects of water temperature, salinity and irradiance on the growth of the toxic dinoflagellate, Gymnodinium catenatum (Graham) isolated from Yeosuhae Bay, Korea. Algae, 19, 293-301 https://doi.org/10.4490/ALGAE.2004.19.4.293
  45. Park, T.G., C.H. Kim and Y. Oshima. 2004. Paralytic shellfish toxin profiles of different geographic population of Gymnodinium catena tum (Dinophyceae) in Korean cosatal waters. PhycoI. Res., 52, 300-305 https://doi.org/10.1111/j.1440-1835.2004.tb00339.x
  46. Provasoli, L., K. Shiraishi and J.R. Lance. 1959. Nutritional idiosyncrasies of Artemia and Tigriopus in monoxenic culture. Ann. NY Sci., 77, 250-261 https://doi.org/10.1111/j.1749-6632.1959.tb36905.x
  47. Smayda, T.J. 1970. The suspension and sinking of phytoplankton in the sea. Oceanogr. Mar. BioI. Ann. Rev., 8, 353-414
  48. Sommer, U. 1989. The role of competition for resources in phytoplankton succession. In: Plankton Ecology. Sommer, U., ed. Springer-Verlag, Berlin, 57-106
  49. Strickland, J.D.H. and T.R. Parsons. 1972. A Practical Handbook of Seawater Analysis, 2nd ed. Bull. Fish. Res. Bd. Canada, 1-310
  50. Tarutani, K. and T. Yamamoto. 1994. Phosphate uptake and growth kinetics of Skeletonema costatum isolated from Hiroshima Bay. J. Fac. Appl. Biol. Sci. Hiroshima Univ., 33, 59-64
  51. Tarutani, K. 1997. Ecophysiological studies on the population dynamics of toxic dinoflagellate Alexandrium tamarense. Ph.D. Thesis, Hiroshima University, Hiroshima, Japan, 1-119
  52. Tilman, D. and S.S. Kilham. 1976. Phosphate and silicate growth and uptake kinetics of the diatoms Asterionella formosa and Cyclotella meneghiniana in batch and semicontinuous culture. J. Phycol., 12, 375-383
  53. Tilman, D. 1982. Resource Competition and Community Structure, Princeton University Press, Princeton, 1-269
  54. Watanabe, M.M., Y. Nakamura, S. Mori and S. Yamochi. 1982. Effect of physico-chemical factors and nutrients on the growth of Heterosigma akashiwo Hada from Osaka Bay, Japan. Jap. J. Phycol., 30, 279-288
  55. Yamaguchi, M. and S. Itakura. 1999. Nutrition and growth kinetics in nitrogen- or phosphorus-limited cultures of the noxious red tide dinoflagellate Gymnodinium mikimotoi. Fish. Sci., 65, 367-373 https://doi.org/10.2331/fishsci.65.367
  56. Yamamoto, T. and K. Tarutani. 1999. Growth and phosphate uptake kinetics of the toxic dinoflagellate Alexandrium tamarense from Hiroshima Bay in the Seto Inland Sea, Jap. Phycol. Res., 47, 27-32 https://doi.org/10.1111/j.1440-1835.1999.tb00280.x
  57. Yamamoto, T. and M. Okai. 2000. Effects of diffusion and upwelling on the formation of red tides. J. Plankton Res., 22, 363-380 https://doi.org/10.1093/plankt/22.2.363
  58. Yamamoto, T., S.J. Oh and Y. Kataoka. 2004. Growth and uptake kinetics for nitrate, ammonium and phosphate by the toxic dinoflagellate Gymnodinium catenatum isolated from Hiroshima Bay, Japan. Fish. Sci., 70, 108-115 https://doi.org/10.1111/j.1444-2906.2003.00778.x
  59. Yoon, Y.H. and S.A. Kim. 1996. Seasonal variations of phytoplankton population and primary productivity in the southern coastal waters of Korea. J. Kor. Environ. Sci., 5, 347-359
  60. Yuki, K. and S. Yoshimatsu. 1987. Morphology of the athecate dinoflagellate Gymnodinium catenatum in culture. Bull. Plankton Soc. Jap., 34, 109-117