Prevalence of Cryptosporidium sp. infection in diarrheic and non-diarrheic humans in Iran

  • Mirzaei, Mohammad (Department of Pathobiology, School of Veterinary Medicine, Shahid Bahonar University of Kerman)
  • Published : 2007.06.30

Abstract

For evaluation of the prevalence of Cryptosporidium sp. infection in diarrheic and non-diarrheic humans in Iran, fecal specimens from diarrheic (n = 129) and non-diarrheic humans (n = 271) were collected and examined for the presence of Cryptosporidium sp. oocysts. The presence of Cryptosporidium sp. oocysts was determined by Ziehl-Neelsen acid-fast staining. Humans were grouped according to their age as follows: younger than 15, 16-25, 26-35, 36-50, and over 51 years. The results showed that the overall prevalence of infection in all 400 samples was 10.8%, but the prevalence (25.6%) in diarrheic humans was higher than that (3.7%) in non-diarrheic humans. Oocysts of Cryptosporidium sp. were detected in the feces of 21.4%, 9.3%, 8.8%, 6.7% and 5.7% of different age groups, respectively. The intensity of oocysts was significantly higher in diarrheic humans than in non-diarrheic ones. There was a significant association between Cryptosporidium sp. infection and occurrence of diarrhea (P < 0.05). The results indicate that Cryptosporidium sp. infection is prevalent in diarrheic humans in Iran.

Keywords

References

  1. Abdel-Messih IA, Wierzba TF, Abu-Elyazeed R, Ibrahim AF, Ahmed SF, Kamal K, Sanders J, French R (2005) Diarrhea associated with Cryptosporidium parvum among young children of the Nile River Delta in Egypt. J Trop Pediatr 51: 154-159 https://doi.org/10.1093/tropej/fmh105
  2. Bezerra FSM, Troiani RM, Parente TML, Paiva ARA, Queiroz RMP, Coelho JR, Oliveira MF (2001) Incidência de criptosporidiose em crianças com desnutricao de moderada a grave internas no IPREDE (Instituto de Prevencao a Desnutricao e a Excepcionalidade) em Fortaleza-CE. Revista da Sociedade Brasileira de Medicina Tropical 34: 300
  3. Casemore DP, Armstrong M, Sands RL (1985) Laboratory diagnosis of cryptosporidiosis. J Clin Pathol 38: 1337- 1341 https://doi.org/10.1136/jcp.38.12.1337
  4. Castro-Hermida JA, Gonzalez-Losada YA, Ares-mazas E (2002) Prevalence of and risk factors involved in the spread of neonatal bovine cryptosporidiosis in Galicia (NW Spain). Vet Parasitol 106: 1-10 https://doi.org/10.1016/S0304-4017(02)00036-5
  5. Chai JY, Lee SH, Guk SM, Lee SH (1996) An epidemiological survey of Cryptosporidium parvum infection in randomly selected inhabitants of Seoul and Jeollanam-do. Korean J Parasitol 34: 113-119 https://doi.org/10.3347/kjp.1996.34.2.113
  6. Chen XM, LaRusso NF (1999) Human intestinal and biliary cryptosporidiosis. World J Gastroenterol 5: 424-429 https://doi.org/10.3748/wjg.v5.i5.424
  7. De la Fuente R, Luzon M, Ruiz-Santa-Quiteria JA, Garcia A, Cid D, Orden JA, Garcia S, Sanz R, Gomez-Bautista M (1999) Cryptosporidium and concurrent infections with other major enteropathogens in 1 to 30-day-old diarrheic dairy calves in central Spain. Vet Parasitol 80: 179-185 https://doi.org/10.1016/S0304-4017(98)00218-0
  8. Fallah M, Haghighi A (1996) Cryptosporidiosis in children with diarrhea submitted to health centers in the west of Iran (Hamadan). Med J Islam Repub Iran 4: 315-317
  9. Fayer R, Morgan U, Upton SJ (2000) Epidemiology of Cryptosporidium: transmission, detection and identification. Int J Parasitol 30: 1305-1322 https://doi.org/10.1016/S0020-7519(00)00135-1
  10. Fayer R, Ungar BLP (1986) Cryptosporidium spp. and cryptosporidiosis. Microbiol Rev 50: 458-483
  11. Hamedi Y, Safa?O, Haidari?M (2005) Cryptosporidium infection in diarrheic children in southeastern Iran. Pediatr Infect Dis J 24: 86-88 https://doi.org/10.1097/01.inf.0000148932.68982.ec
  12. Hoghooghi-Rad N (1994) Some epidemiological aspects of cryptosporidiosis in Ahvaz, Capital of Khoozestan province, Islamic Republic of Iran. Med J Islam Repub Iran 1: 17-22
  13. Houpt E, Bushen OY, Sam NE, Kohli A, Asgharpour A, Ng CT, Calfee DP, Guerrant RL, Maro V, Ole-Nguyaine S, Shao JF (2005) Short report: asymptomatic Cryptosporidium hominis infection among human immunodeficiency virus-infected patients in Tanzania. Am J Trop Med Hyg 73: 520-522
  14. Khoshzaban F, Dalimi-Asl A (1998) Epidemiological study on Cryptosporidiosis in diarrheatic children. Sci Res J Shahed Univ (Iran) 19: 26-21
  15. Lee JK, Song HJ, Yu JR (2005) Prevalence of diarrhea caused by Cryptosporidium parvum in non-HIV patients in Jeollanam-do, Korea. Korean J Parasitology 43: 111-114 https://doi.org/10.3347/kjp.2005.43.3.111
  16. Lefay D, Naciri M, Poirier P, Chermette R (2000) Prevalence of Cryptosporidium infection in calves in France. Vet Parasitol 89: 1-9 https://doi.org/10.1016/S0304-4017(99)00230-7
  17. Maleki F, Sadegh-Hasani S (2003) Prevalence of cryptosporidiosis in students of elementary schools in the west Tehran-Iran from 1999-2001. J Iran Univ Med Sci 33: 110-105
  18. Meamar AR, Rezaian M, Rezaie S, Mohraz M, Mohebali M, Mohammad K, Golestan B, Guyot K, Dei-Cas E (2006) SSU- rRNA Gene Analysis of Cryptosporidium spp. in HIV Positive and Negative Patients. Iranian J Publ Hlth 35: 1-7
  19. Naciri M, Lefay MP, Mancassola R, Poirier P, Chermette R (1999) Role of Cryptosporidium parvum as a pathogen in neonatal diarrhea complex in suckling and dairy calves in France. Vet Parasitol 85: 245-257 https://doi.org/10.1016/S0304-4017(99)00111-9
  20. Nahrevanian H, Assmar M, Samin MG (2007) Cryptosporidiosis among immunocompetent patients with gastroenteritis in Iran: a comparison with other enteropathogenic parasites. J Microbiol Immunol Infect 40: 154-156
  21. Nouri M, Moghadam A, Haghighatnia H (1991) Cryptosporidium infection in human diarrhea patients in West Azerbaijan, Iran. Med J Islam Repub Iran 2: 35-38
  22. O'Donoghue PJ (1995) Cryptosporidium and cryptosporidiosis in man and animals. Int J Parasitol 25: 139-195 https://doi.org/10.1016/0020-7519(94)E0059-V
  23. Park JH, Kim HJ, Guk SM, Shin EH, Kim JL, Rim HJ, Lee SH, Chai JY (2006) A survey of cryptosporidiosis among 2,541 residents of 25 coastal islands in Jeollanam-Do (Province), Republic of Korea. Korean J Parasitol 44: 367- 72 https://doi.org/10.3347/kjp.2006.44.4.367
  24. Perch M, Sodemann M, Jakobsen MS, Valentiner-Branth P, Steinsland H, Fischer TK, Lopes DD, Aaby P, Molbak K (2001) Seven years' experience with Cryptosporidium parvum in Guinea-Bissau, West Africa. Ann Trop Paediatr 21: 313-318 https://doi.org/10.1080/07430170120093490
  25. Seo M, Huh S, Chai JY, Yu JR (2001) An epidemiological survey on Cryptosporidium parvum infection of inhabitants in Chorwon-gun, Kangwon-do. Korean J Parasitol 39: 201-203 https://doi.org/10.3347/kjp.2001.39.2.201
  26. Sevinc F, Irmak K, Sevinc M (2003) The prevalence of Cryptosporidium parvum infection in the diarrhoiec and non- diarrheic calves. Revue Med Vet 154: 357-361
  27. Sturdee AP, Chalmers RM, Bull SA (1999) Detection of Cryptosporidium oocysts in wild mammals of mainland Britain. Vet Parasitol 80: 273-280 https://doi.org/10.1016/S0304-4017(98)00226-X
  28. Sulaiman IM, Hira PR, Zhou L, Al-Ali FM, Al-Shelahi FA, Shweiki HM, Iqbal J, Khalid N, Xiao L (2005) Unique endemicity of cryptosporidiosis in children in Kuwait. J Clin Microbiol 43: 2805-2809 https://doi.org/10.1128/JCM.43.6.2805-2809.2005
  29. Uga S, Matsuo J, Kono E, Kimura K, Inoue M, Rai SK, Ono K (2000) Prevalence of Cryptosporidium parvum infection and pattern of oocyst shedding in calves in Japan. Vet Parasitol 94: 27-32 https://doi.org/10.1016/S0304-4017(00)00338-1
  30. Yu JR, Lee JK, Seo M, Kim SI, Sohn WM, Huh S, Choi HY, Kim TS (2004) Prevalence of cryptosporidiosis among the villagers and domestic animals in several rural areas of Korea. Korean J Parasitol 42: 1-6 https://doi.org/10.3347/kjp.2004.42.1.1