Evaluation for Cytopreservability of Manual Liquid-Based Cytology $Liqui-PREP^{TM}$ and its Application to Cerebrospinal Fluid Cytology: Comparative Study with Cytospin

수기 액상세포검사 $Liqui-PREP^{TM}$의 세포보존력 평가 및 뇌척수액 세포검사에의 적용: 세포원심분리법과의 비교

  • Park, Gyeong-Sin (Department of Hospital Pathology, College of Medicine, The Catholic University of Korea) ;
  • Lee, Kyung-Ji (Department of Hospital Pathology, College of Medicine, The Catholic University of Korea) ;
  • Jung, Chan-Kwon (Department of Hospital Pathology, College of Medicine, The Catholic University of Korea) ;
  • Lee, Dae-Hyoung (Department of Pediatrics, College of Medicine, The Catholic University of Korea) ;
  • Cho, Bin (Department of Pediatrics, College of Medicine, The Catholic University of Korea) ;
  • Lee, Youn-Soo (Department of Hospital Pathology, College of Medicine, The Catholic University of Korea) ;
  • Shim, Sang-In (Department of Hospital Pathology, College of Medicine, The Catholic University of Korea) ;
  • Lee, Kyo-Young (Department of Hospital Pathology, College of Medicine, The Catholic University of Korea) ;
  • Kang, Chang-Suk (Department of Hospital Pathology, College of Medicine, The Catholic University of Korea)
  • 박경신 (가톨릭대학교 의과대학 병원병리학교실) ;
  • 이경지 (가톨릭대학교 의과대학 병원병리학교실) ;
  • 정찬권 (가톨릭대학교 의과대학 병원병리학교실) ;
  • 이대형 (가톨릭대학교 의과대학 소아과학교실) ;
  • 조빈 (가톨릭대학교 의과대학 소아과학교실) ;
  • 이연수 (가톨릭대학교 의과대학 병원병리학교실) ;
  • 심상인 (가톨릭대학교 의과대학 병원병리학교실) ;
  • 이교영 (가톨릭대학교 의과대학 병원병리학교실) ;
  • 강창석 (가톨릭대학교 의과대학 병원병리학교실)
  • Published : 2007.03.30

Abstract

Cerebrospinal fluid (CSF) cytology is an effective tool for evaluating diseases involving the central nervous system, but this technique is usually limited by its low cellularity and poor cellular preservation. Here we compared the manual liquid-base $Liqui-PREP^{TM}$ (LP) to the cytospin (CS) with using a mononuclear cell suspension and we applied both methods to the CSFs of pediatric leukemia patients. The cytopresevability, in terms of cell yield and cell size, and the clinical efficacy were evaluated. When 2000 and 4000 mononuclear cells were applied, LP was superior to CS for the cell yield, 16.8% vs 1.7% (P=0.001) and 26.2% vs 3.5% (P=0.002), respectively. The mean size of the smeared cells was 10.60 ${\mu}m$ in the CS, 5.01 ${\mu}m$ in the LP and 6.50 ${\mu}m$ in the direct smear (DS), and the size ratio was 1.7 (CS to DS), 0.8(LP to DS) and 2.1 (CS to LP), respectively. As compared to the cells in the DS, the cells in the CS were significantly enlarged, but those in the LP were slightly shrunken. Upon application to 109 CSF samples, 4 were diagnosed as positive for leukemia (positive), 4 had atypical cells and 101 were negative by CS; 6 were positive, one had atypical cells and 102 were negative by LP. For six cases, in which 4 were positive for leukemia and 2 of 4 had atypical cells by CS, they were positive by LP and they were also confirmed as positive according to the follow-up study. Three cases diagnosed as atypical cells (two by CS and one by LP), were confirmed as negative. In conclusion, these results suggest that LP is superior to CS for the cytopresevability and for rendering a definite diagnosis of cerebrospinal fluid.

Keywords

References

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