Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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An Efficient Method for the Extraction of Astaxanthin from the Red Yeast Xanthophyllomyces dendrorhous

  • Choi, Seok-Keun (Department of Biotechnology, School of Life Sciences and Biotechnology, Korea University) ;
  • Kim, Jeong-Hwan (Department of Physiology, College of Medicine, Pusan National University) ;
  • Park, Young-Sam (Department of Biotechnology, School of Life Sciences and Biotechnology, Korea University) ;
  • Kim, Young-Jin (Korea Food Research Institute) ;
  • Chang, Hyo-Ihl (Department of Biotechnology, School of Life Sciences and Biotechnology, Korea University)
  • Published : 2007.05.31
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Abstract

This study investigated an efficient method for the extraction of astaxanthin from the red yeast Xanthophyllomyces dendrorhous. The extraction process comprised three steps: 1) cultivating the yeast; 2) treating the yeast culture suspension with microwaves to destroy the cell walls and microbodies; and 3) drying the yeast and extracting the astaxanthin pigment using ethanol, methanol, acetone, or a mixture of the three as the extraction solvent. Ultimately, various treatment tests were performed to determine the conditions for optimal pigment extraction, and the total carotenoid and astaxanthin contents were quantified. A frequency of 2,450 MHz, an output of 500 watts, and irradiation time of 60 s were the most optimum conditions for yeast cell wall destruction. Furthermore, optimal pigment extraction occurred when using a cell density of 10g/l at $30^{\circ}C$ over 24 h, with a 10% volume of ethanol.

Keywords

References

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