Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Involvement of p53-Mediated Mitochondrial Stress in the Apoptosis Induced by Flavonoids Purified from Rhus verniciflua Stokes in Human Osteosarcoma Cells

  • Chung, Song-Woo (Laboratory of Cell Biology in Department of Orthodontins and Institute of Oral Bioscience, Chonbuk National University) ;
  • Lee, Seung-Ah (Department of Nursing, Chonnam Techno College, Department of Bioactive Materials, Research Center of Bioactive Materials, Chonbuk National University) ;
  • Park, Jong-Sun (Laboratory of Cell Biology in Department of Orthodontins and Institute of Oral Bioscience, Chonbuk National University) ;
  • Ryu, Kwon-Woo (Laboratory of Cell Biology in Department of Orthodontins and Institute of Oral Bioscience, Chonbuk National University) ;
  • Jang, Mun-Ju (Laboratory of Cell Biology in Department of Orthodontins and Institute of Oral Bioscience, Chonbuk National University) ;
  • Park, Song-Soo (Laboratory of Cell Biology in Department of Orthodontins and Institute of Oral Bioscience, Chonbuk National University) ;
  • Lee, Choon-Bong (Laboratory of Cell Biology in Department of Orthodontins and Institute of Oral Bioscience, Chonbuk National University) ;
  • Kim, Jong-Ghee (Laboratory of Cell Biology in Department of Orthodontins and Institute of Oral Bioscience, Chonbuk National University) ;
  • Jeon, Young-Mi (Laboratory of Cell Biology in Department of Orthodontins and Institute of Oral Bioscience, Chonbuk National University) ;
  • Lee, Jeong-Chae (Laboratory of Cell Biology in Department of Orthodontins and Institute of Oral Bioscience, Chonbuk National University, Department of Bioactive Materials, Research Center of Bioactive Materials, Chonbuk National University)
  • Published : 2007.03.31
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Abstract

Dietary flavonoids have antioxidant and antitumor promoting effects. Rhus verniciflua Stokes (RVS) is a flavonoid-rich herbal medicine and has long been used as a food additive and an antitumor agent in Korea. Previous study demonstrated that a purified flavonoid fraction prepared from RVS, herein named RCMF (the RVS chloroform-methanol fraction), exhibited growth inhibition and induced apoptosis in human osteosarcoma(HOS) cells. This study evaluated if p53-mediated pathway is associated with the RCMF-induced apoptosis in HOS cells. RCMF was shown to be capable of inducing apoptosis of the cells, as expected, and transparently increased p53 expression in the cells. However, the RCMF-induced cytotoxicity was suppressed by transfecting the cells with antisense p53 oligonucleotide, which also inhibited the decrease of Bcl-2 and the increase of Bax in mitochondria, and the release of cytochrome c into cytosol. This finding suggests that p53-mediated mitochondrial stress is required for RCMF-induced apoptosis in HOS cells.

Keywords

References

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