Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Conditioning of the Extraction of Acidic Polysaccharide from Red Ginseng Marc

홍삼박으로부터 산성다당체의 최적 추출 조건 분석

  • Chang, Eun-Ju (Bio-Food and Drug Research Center, Konkuk University) ;
  • Park, Tae-Kyu (Bio-Food and Drug Research Center, Konkuk University) ;
  • Han, Yong-Nam (Natural Products Research Institute, Seoul National University) ;
  • Hwang, Keum-Hee (Bio-Food and Drug Research Center, Konkuk University)
  • 장은주 (건국대학교 바이오식의약연구센터) ;
  • 박태규 (건국대학교 바이오식의약연구센터) ;
  • 한용남 (서울대학교 천연물과학연구소) ;
  • 황금희 (건국대학교 바이오식의약연구센터)
  • Published : 2007.03.31
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Abstract

This study was carried out to investigate the optimum conditions for extraction of acidic polysaccharides from red ginseng marc produced by manufacturing alcoholic extract from red ginseng. Method of carbazole-sulfuric acid was applied to determine the amount of acidic polysaccharides in red ginseng marc. The amounts of acidic polysaccharides in water extract of red ginseng marc were increased with increasing extraction temperature. The contents of acidic polysaccharides were not significantly different despite of the extraction time increasing from 6 hours to 48 hours. The contents of starch in water-extract of red ginseng marc were increased with increasing extraction temperature. The starch amounts in water extract of red ginseng marc extracted for 48 hours were increased. The yields of polysaccharide precipitated from water-extract of red ginseng marc were increased with increasing extraction temperature. The hydration rate of acidic polysaccharides and starch from water-extract of red ginseng marc were decreased with increasing extraction temperature. The contents of starch were not significantly different despite of the extraction time increasing from 6 hours to 48 hours at $8^{\circ}C$. However, the rehydration rate of acidic polysaccharide for 48 hours were decreased at $8^{\circ}C$. The rehydration rate of acidic polysaccharide and starch extracted from 6 hours to 24 hours at $25^{\circ}C$ were not significantly different, but those extracted for 48 hours were increased. From the above results, we suggest that by altering the extraction conditions in red ginseng marc it is possible to develop optimum conditions for extraction that modulate the proportions of acidic polysaccharide and starch.

Keywords

References

  1. Brekhman, I. I. and Dardymov, I. V. (1969) New substances of plant origin which increase nonspecpic resistance. Ann. Rev. Pharmacol. 9: 419-430 https://doi.org/10.1146/annurev.pa.09.040169.002223
  2. Park, C. K., Jeon, B. S. and Yang, J. W. (2003) The chemical components of korean ginseng. Food industry and Nutrition. 8: 10-23
  3. Konno, C., Sugiyama, K., Kano, M., Takahashi, M. and Hikino, H. (1984) Isolation and hypoglycemic activity of panaxans A, B, C, D and E, glycans of Panax ginseng roots. Planta Medica. 50: 434-436 https://doi.org/10.1055/s-2007-969757
  4. Oshima, Y., Konno, C. and Hikino, H. (1985) Isolation and hypoglycemic activity of panaxans I, J, K and L, glycans of Panax ginseng roots. J. Ethnopharmacol. 14: 255-259 https://doi.org/10.1016/0378-8741(85)90091-1
  5. Konno, C., Murakami, M., Oshima, Y. and Hikino, H. (1985) Isolation and hypoglycemic acitivity of panaxans Q, R, S, T and U, glycans of Panax ginseng roots. J. Ethnopharmacol. 14: 69-74 https://doi.org/10.1016/0378-8741(85)90030-3
  6. Gao, Q. P., Kiyohara, H., Cyong, J. C. and Yamada, H. (1991) Chemical properties and anti-complementary activities of heteroglycans from the leaves of Panax ginseng. Plant Medica. 57: 132-136 https://doi.org/10.1055/s-2006-960049
  7. Kim, K. H., Jung, I. S., Chung, H. Y., Jo, S. K. and Yun, Y. S. (1997) Preclinical evaluation of polysaccharides extracted from korean red ginseng as an antineoplastic immunostimulator. Korean J. Ginseng Sci. 21: 78-84
  8. Park, K. M., Jeong, T. C., Kim, Y. S., Shin, H. J., Nam, K. Y. and Park, J. D. (2000) Immunomodulatory effect of acidic polysaccharide from korean red ginseng (Panax ginseng). Natural product sciences. 6: 31-35
  9. Park, K. M., Kim, Y. S., Jeong, T. C., Joe, C. O., Shin, H. J., Lee, Y. H., Nam, K. Y. and Park, J. D. (2001) Nitric oxide is involved in the immunomodulating acitivies of acidic polysaccharide from Panax ginseng. Planta Med. 67: 122- 126 https://doi.org/10.1055/s-2001-11508
  10. Kim, Y. S., Park, K. M., Shin, H. J., Song, K. S., Nam, K. Y. and Park, J. D. (2002) Anticancer activities of red ginseng acidic polysaccharide by acitivation of macrophasges and natural killer cells. Yakhak Hoeji. 46: 113-119
  11. Kwak, Y. S., Kim, Y. S., Shin, H. J., Song, Y. B. and Park, J. D. (2003) Anticancer activities by combined treatment of red ginseng acidic polysaccharide (RGAP) and anticancer agents. J. Ginseng Res. 27: 47-51 https://doi.org/10.5142/JGR.2003.27.2.047
  12. Shin, H. J., Kim, Y. S., Kwak, Y. S., Song, Y. B., Kyung, J. S., Wee, J. J. and Park, J. D. (2004) A futher study on the inhibition of tumor growth and metastasis by red ginseng acidic polysaccharide (RGAP). Natural Product Sciences. 10: 284-288
  13. Kong, Y. C., Fong, W. P., Song, M. E., Ng, K. H., Ho, D. D. and Ng, P. C. (1990) Partial purification and characterization of a glycoprotein factor from fresh ginseng. Korean J. Ginseng Sci. 14: 221-227
  14. Lee, S. D., Kameda, K., Takaku, T., Sekiya, K., Hirose, K., Ohtani, K., Tanaka, O. and Okuda, H. (1990) Effect of acidic polysaccharide of red ginseng on lipolitic aciton of toxohormone- L from cancerous ascites fluid. Korean J. Gkinseng Sci. 14: 1-5
  15. Okuda, H., Lee, S. D., Matsuura, Y., Zheng, Y., Sekiya, K., Takaku, T., Kamdea, K., Hirose, K., Ohtani, K. and Tanaka, O. (1990) Biological activities of non-saponin compounds isolated from Korean red ginseng. Korean J. Ginseng Sci. 14: 157-161
  16. Lee, J. K., Choi, J. W., Kim, S. H., Kim, H. K. and Han, Y. N. (1998) Biological activity of acidic polysaccharide of Korean red ginseng. I. Effects on alcohol detoxification system in the liver of accohol-intoxicated rats. J. Ginseng Res. 22: 260-266
  17. Lee, J. K., Choi, J. W., Kim, H. K. and Han, Y. N. (1999) Biological activity of acidic polysaccharide of korean red ginseng. II. Effects on hyperlipidemia induced by alcohol. J. Ginseng Res. 23: 8-12
  18. Lee, J. K., Choi, J. W., Kim, S. H., Kim, H. K. and Han, Y. N. (1998) Biological activity of acidic polysaccharide of korean red ginseng. III. Effects on metabolizing activitites in acetaminophen-treated rats. J. Ginseng Res. 22: 167-273
  19. Miller, G. L. (1972) Use of dinitro salycylic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426-428 https://doi.org/10.1021/ac60147a030
  20. Bitter, T. and Muir, H. M. (1962) A modified uronic acid carbazole reaction. Anal. Biochem. 4: 330-334 https://doi.org/10.1016/0003-2697(62)90095-7
  21. Do, J. H., Lee, H. O., Lee, S. K., Noh, K. B., Lee, S. D. and Lee, K. S. (1993) Comparisons of acidic polysaccharide content in various ginseng species and parts. Korea J. Ginseng Sci. 17: 145-147
  22. Lee, J. W. and Do, J. H. (2002) Extraction condition of acidic polysaccharide from Korean red ginseng marc. J. Ginseng Res. 26: 202-205 https://doi.org/10.5142/JGR.2002.26.4.202
  23. Do, J. H., Lee, H. O., Lee, S. K., Jang, J. K., Lee, S. D. and Sung, H. S. (1993) Colorimetric determination of acidic polysaccharide from Panax ginseng, its extraction condition and stability. Korea J. Ginseng Sci. 17: 139-144
  24. Belitz, H. D. and Grosch, W. (Eds) (1999) Food chemistry. 301. Apringer-Verlag, Berlin