KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Influence of Surfactant on the Hydrolysis of Used Newspaper

폐 신문지의 가수분해에 미치는 계면활성제의 영향

  • Kim, Sung-Bae (Department of Chemical & Biological Engineering and ERI, Gyeongsang National University) ;
  • Shin, Hae-Joong (Department of Chemical & Biological Engineering and ERI, Gyeongsang National University) ;
  • Kim, Chang-Joon (Department of Chemical & Biological Engineering and ERI, Gyeongsang National University) ;
  • Bak, Young-Cheol (Department of Chemical & Biological Engineering and ERI, Gyeongsang National University)
  • 김성배 (경상대학교 공과대학 생명화학공학과 및 공학원) ;
  • 신해중 (경상대학교 공과대학 생명화학공학과 및 공학원) ;
  • 김창준 (경상대학교 공과대학 생명화학공학과 및 공학원) ;
  • 박영철 (경상대학교 공과대학 생명화학공학과 및 공학원)
  • Published : 2007.02.28
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Abstract

The effect of surfactant on the hydrolysis of used newspaper was investigated. The most suitable surfactant for the pretreatment stage was found to be NP-series surfactants among 9 kinds of non ionic surfactants. Process parameters such as surfactant concentration, mixing speed, pretreatment temperature and time were tested to optimize for maximum digestibility and 0.5%, 100rpm, 30$^{\circ}C$, and 1 h were found to be optimum, respectively. In order to maximize digestibility, substrate was pretreated with NP-20 and then the pretreated substrate was hydrolyzed by adding TW-80. The effect of surfactant on the hydrolysis of previously surfactant-pretreated newspaper was marginal. Therefore, the digestibility with the addition order of enzyme and surfactant was investigated by using surfactant only in hydrolysis stage. The results show that digestibility was more lowered as the surfactant addition after adding enzyme to substrate was more delayed.

신문지와 같은 폐지의 가수분해에 미치는 계면활성제의 영향을 조사하였다. 전처리 공정에 적합한 계면활성제는 사용한 9종의 비이온 계면활성제 중 NP 계열 계면활성제가 가장 좋은 전처리 효과를 나타내었다. NP-20을 사용하여 전처리 공정을 최적화한 결과 계면활성제 농도, 교반속도, 전처리 온도와 시간이 0.5%, 100 rpm, 30$^{\circ}C$와 1시간일 때 최적조건이었다. 당화율을 최대화하기 위하여 기질을 NP-20으로 전처리하고 전처리된 기질을 TW-80을 첨가하여 가수분해하였다. 계면활성제를 사용하여 전처리된 기질을 계면활성제를 첨가하여 가수분해하면 가수분해에서 계면활성제의 효과가 거의 없었다. 그래서 가수분해 공정에서만 계면활성제를 사용하여 효소와 계면활성제의 첨가 순서에 따른 당화율을 조사하였다. 조사결과 기질에 효소를 넣고 난 후 계면활성제의 첨가시기가 늦을수록 당화율이 낮아지는 경향을 나타내었다.

Keywords

References

  1. Bergeron, P. W. and C. J. Riley (1991), Wastepaper as a Feedstock for Ethanol Production, NREL/TP-232-4237, National Renewable Energy Laboratory, Golden, Colorado, USA
  2. Korea Paper Manufacture's Association (2003), Report for Production and Treatment of Paper Mill Waste, Seoul, Korea
  3. Scott, C. D., B. H. Davison. T. C. Scott, J. Woodward, C. Dees, and D. S. Rothrock (1994), An Advanced Bioprocessing Concept for the Conversion of Waste paper to Ethanol, Appl. Biochem. Biotechnol. 45/46, 641-653 https://doi.org/10.1007/BF02941836
  4. Holtzapple, M. T., J. E. Lundeen, R. Sturgis, J. E. Lewis, and B. E. Dale (1992), Pretreatment of Lignocellulosic Municipal Solid Waste by Ammonia Fiber Explosion (AFEX), Appl, Biochem. Biotechnol. 34/35, 5-21 https://doi.org/10.1007/BF02920530
  5. Moon, N. K. and S. D. Kim (2001), Pretreatment of Used Newspaper to Increase Enzymatic Digestibility, Korean J. Biotechnol. Bioeng. 16(5), 446-451
  6. Kim, S. B. and N. K. Moon (2003), Enzymatic Digestibility of Used Newspaper Treated with Aqueous Ammonia-Hydrogen Peroxide Solution, Appl. Biochem. Biotechnol. 105/108, 365-373
  7. Kim, S. B. and J. W. Chun (2004), Enhancement of Enzymatic Digestibility of Recycled Newspaper by Addition of Surfactant in Ammonia-Hydrogen Peroxide Pretreatment, Appl. Biochem. Biotechnol. 113/116, 1023-1031
  8. Kim, S. B. and J. W. Chun (2004), Pretreatment for Enzymatic Hydrolysis of Used Newspaper, In Lignocellulosic Biodegradation, Saha. B. C. and Hayashi, K., Eds., p36, ACS Books, Washington, USA
  9. Khan, A. W., J. Labrie, and J. McKeown (1987), Electron Beam Irradiation Pretreatment and Enzymatic Saccharification of Used Newsprint and Paper Mill Wastes, Radiat. Phys. Chem. 29, 117-120
  10. Zheng, Y., H. Lin, and G. T. Tsao (1998), Pretreatment for Cellulose hydrolysis by Carbon Dioxide Explosion, Biotechnol. Prog. 14, 890-896 https://doi.org/10.1021/bp980087g
  11. Kim, J. S., Y. Y. Lee, and S. C. Park (2000), Pretreatment of Wastepaper and Paper Mill Sludge by Aqueous Ammonia and Hydrogen Peroxide, Appl. Biochem. Biotechnol. 84/86, 129-139 https://doi.org/10.1385/ABAB:84-86:1-9:129
  12. Kim, S. B, H. J. Kim, and C. J. Kim (2006), Enhancement of the Enzymatic Digestibility of Waste Newspaper using Tween, Appl. Biochem. Biotechnol. 131, 486-495
  13. Kim, S. B., H. J. Kim, and C. J. Kim (2006), Effect of Nonionic Surfactant on the Pretreatment and Enzymatic Hydrolysis of Recycled Newspaper, submitted in Process Biochemistry
  14. Eriksson, T., J. Borjesson, and F. Tjerneld (2002), Mechanism of Surfactant Effect in Enzymatic Hydrolysis of Lignocellulose, Enzyme Micro. Technol. 6112, 1-12
  15. Helle, S. S., S. J. B. Duff, and D. G. Cooper (1993), Effect of Surfactants on Cellulose Hydrolysis, Biotechnol. Bioeng. 42, 611-617 https://doi.org/10.1002/bit.260420509
  16. Karr, W. E. and M. T. Holtzapple (1998), Benefits from Tween During Enzymic Hydrolysis of Corn Stover, Biotechnol. Bioeng. 59, 419-427 https://doi.org/10.1002/(SICI)1097-0290(19980820)59:4<419::AID-BIT4>3.0.CO;2-J
  17. Wu, J. and L. Ju (1998), Enhancing Enzymatic Saccharification of Waste Newsprint by Surfactant Addition, Biotechnol. Prog. 14, 649-652 https://doi.org/10.1021/bp980040v
  18. Castanon, M. and C. R. Wilke (1981), Effect of the Surfactant Tween 80 on Enzymatic Hydrolysis of Newspaper, Biotechnol. Bioeng. 23, 1365-1372 https://doi.org/10.1002/bit.260230615
  19. http://www.ott.doe.gov/biofuels/analytical_methods.html
  20. Lassus, A. (2006), Deinking Chemistry, In Recycled Fiber and Deinking Book 7, L. Gottsching, Ed., p241, Gummerus Printing, Filand