KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Reuse of Eluent by Controlling its Specific Gravity during the Chromatographic Purification of Paclitaxel

크로마토그래피를 이용한 Paclitaxel 정제에서 비중 조절에 의한 Eluent 재사용

  • Kim, Jin-Hyun (Department of Chemical Engineering, Kongju National University)
  • 김진현 (공주대학교 화학공학부)
  • Published : 2007.10.30
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Abstract

In this study, the feasibility of reusing the eluent was confirmed by monitoring its specific gravity during the chromatographic purification of paclitaxel from plant cell cultures. The specific gravity of the eluent (methanol/water = 70/30, v/v) was measured prior to its elution through the hydrophobic resin column. The measurement showed a specific gravity of 0.853. The discharged eluent from the column outlet was first evaporated under vacuum pressure. The evaporated eluent was collected and condensed into a liquid eluent again, followed by the HPLC analysis in order to check the presence of any trace of impurity. Even if the specific gravity of the liquid eluent is varied from 0.853 as a result of the evaporation and condensation, the eluent can still be reused after it specific gravity is adjusted by the addition of methanol or water. The reuse of the eluent resulted in the paclitaxel yield of 86% with a purity of 95% which were closely similar to those of before the eluent reuse. These results indicate that the strategy of reusing the eluent on the basis of the specific gravity analysis was successfully implemented in this study.

본 연구에서는 식물세포배양으로부터 회수한 식물세포, 즉 biomass로부터 항암제 paclitaxel를 정제하기 위한 크로마토그래피 공정에서 컬럼을 통하여 용리되는 eluent의 비중 (specific gravity) 조절에 의한 eluent 재사용 방법을 개발하였다. 크로마토그래피 조업 전에 eluent (methanol/water = 70/30, v/v)의 비중을 측정한 결과 0.853을 얻었다. 컬럼으로부터 분취한 용액을 증발 건조하여 건조된 제품과 증발된 용액인 eluent를 각각 회수하였다. HPLC 분석을 통하여 증발/회수된 eluent에 불순물이 포함되어 있지 않음을 확인하고, 회수된 eluent의 비중을 측정하여 비중이 0.853에서 벗어났을 경우에는 메탄올 또는 물을 첨가하여 eluent의 비중을 0.853으로 조정하여 eluent로 재사용하였다. 회수된 eluent를 재사용하여 크로마토그래피 정제를 실시한 결과, 고순도 ($\sim$95%), 고수율 ($\sim$86%)의 paclitaxel을 얻을 수 있었다. 이는 eluent를 재사용하지 않을 경우, 즉 재사용 전과 동일한 수준의 결과로 비중 조절에 의한 eluent의 재사용이 성공적임을 알 수 있었다.

Keywords

References

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