KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Detection of C-Reactive Protein Using Direct-binding Quartz Crystal Microbalance Immunosensor

직접결합방식 수정진동자 면역센서에 의한 C-Reactive Protein 검출

  • Kim, N. (Food Nano-Biotechnology Research Center, Korea Food Research Institute) ;
  • Kim, D.K. (Food Nano-Biotechnology Research Center, Korea Food Research Institute) ;
  • Cho, V.J. (Food Nano-Biotechnology Research Center, Korea Food Research Institute)
  • Published : 2007.12.31
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Abstract

A prognostic indicator of coronary heart disease, C-reactive protein, was tried to be determined by a batch-type quartz crystal microbalance immunosensor. The sensor was operated by direct-binding mode and the optimum concentration for the corresponding antibody for immobilization was $50{\mu}g/ml$. The reaction buffer for the system was 0.1 M sodium phosphate (pH 7.0) and system operation was performed in the order of baseline stabilization, analyte addition and measurement, and regeneration of the sensor chip with 10 mM NaOH. When plotted in double-logarithmic scale, the sensor showed a linear detection range of 0.27-106.00 nM for rat C-reactive protein with the limit of detection of 0.53 nM. It also showed a good reusability.

관상심장질환 바이오마커의 하나인 CRP를 batch형 수정진동자 면역센서에 의하여 다음과 같이 분석하였다. 센서시스템의 작동은 직접결합방식에 의하여 행하였으며 CRP에 대한 항체의 최적 고정화농도는 $50{\mu}g/ml$이었다. 시스템의 반응완충용액으로 0.1 M 인산완충용액 (pH 7.0)을 사용하였고 시스템 작동은 baseline 안정화, 시료첨가 및 측정, 10 mM NaOH에 의한 센서 칩 재생의 순으로 행하였다. 이중로그척도로 표시하였을 때 0.27-106.00 nM 범위의 쥐 유래 CRP에 대하여 센서반응과 직선상의 관계를 이루었으며 센서의 검출한계는 0.53 nM이었고 재사용성도 양호하였다.

Keywords

References

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