Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Suppressive Effect of Solanum Iyratum Aqueous Extract Via Down-regulation of $TNF-{\alpha}$ and $IFN-{\gamma}$ Production on Collagen-induced Arthritis in Mice

  • Kim, Seung-Hyung (Institute of Traditional Medicine & Bioscience, Daejeon University) ;
  • Seo, Chang-Woo (Department of Herbology and Prescription, Sangji University) ;
  • Kim, Chang-Min (Department of Herbology and Prescription, Sangji University) ;
  • Kim, Yang-Jin (Department of Herbology and Prescription, Sangji University) ;
  • Lee, Boo-Kyun (Department of Herbology and Prescription, Sangji University) ;
  • Choi, Yong-Sun (Department of Herbology and Prescription, Sangji University) ;
  • Oh, Hwang (Department of Herbology and Prescription, Sangji University) ;
  • Yoon, Ho-Sok (Department of Herbology and Prescription, Sangji University) ;
  • Lee, Seon-Goo (Department of Pathology, College of Oriental Medicine, Sangji University) ;
  • Lee, Jang-Cheon (Department of Herbology and Prescription, Sangji University) ;
  • Lee, Young-Cheal (Department of Herbology and Prescription, Sangji University)
  • Published : 2007.10.25
Download PDF
⟨ Previous Next ⟩

Abstract

Solanum Iyratum Thunb (Solanaceae) has multiple applications in korean traditional medicine because of its cytotoxic, immunological and anti-inflammatory activities. However, no study on the anti-arthritic activity of Solanum Iyratum Thunb has been reported in vivo. Rheumatoid arthritis (RA) is a systemic autoimmune disease with chronic inflammation characterized by hyperplasia of synovial cells in affected joints, which ultimately leads to the destruction of cartilage and bone. Cytokine production were assessed during CIA(collagen-induced arthritis) model mice in lymph node (LN), in knee joint and spleen, using ELISA. DBA/1j mice were immunized with bovine type II collagen. After a second collagen immunization, mice were treated with Solanum Iyratum Thunb (SLT) orally at 400, 200 mg/kg once a day for 4 weeks. The severity of arthritis within the knee joints was evaluated by histological assessment of cartilage destruction and pannus formation. SLT significantly suppressed the progression of CIA and inhibited the production of TNF-alpha and IFN-gamma in serum and spleen cell culture supernatant. The erosion of cartilage was dramatically reduced in mouse knees after treatment with SLT. In conclusion, our results demonstrates that SLT significantly suppressed the progression of CIA. This action was characterized by suppression of arthritis index, cartilage erosion and synovial cell infiltration and the decreased production of $TNF-{\alpha}$, $IFN-{\gamma}$, CD4+, CD19+, CD3+/CD69+ cells (in lymph node), CD11b+/Gr-1 + (in knee joint).

Keywords

References

  1. Koch, A.E. Angiogenesis: implications for rheumatoid arthritis. ARTHRITIS & RHEUMATISM 41: 951-962, 1998 https://doi.org/10.1002/1529-0131(199806)41:6<951::AID-ART2>3.0.CO;2-D
  2. Wolfe, F., Hawley, D.J. The longterm outcomes of rheumatoid arthritis: work disability: a prospective 18 year study of 823 patients. Journal of Rheumatology 25: 2108-2117, 1998
  3. Feldmann, M., Brennan, F.M., Maini, R.N. Role of cytokines in rheumatoid arthritis. Annu Rev Immunol 14: 397-440, 1996 https://doi.org/10.1146/annurev.immunol.14.1.397
  4. Chinh, N., Tran, Steven, K. Lundy, David, A. Fox. Synovial biology and T cells in rheumatoid arthritis. Pathophysiology 12: 183-189, 2005 https://doi.org/10.1016/j.pathophys.2005.07.005
  5. Mary, E., Morgan, Roel of Flierman, Leonie M. van Duivenvoorde, Hendrik J. Witteveen, Willem van Ewijk, Jacob M. van Laar, Rene R.P. de Vries, and Rene E.M. Toes. Effective Treatment of Collagen-Induced Arthritis by Adoptive Transfer of CD25 Regulatory T Cells. arthritis & rheumatism 52: 2212-2221, 2005 https://doi.org/10.1002/art.21195
  6. Kim, H.M., Kim, M.J., Li, E., Lyu, Y.S., Hwang, C.Y., An, N.H. The nitric oxide-producing properties of Solanum lyratum. Journal of Ethopharmacology 67: 163-169, 1999 https://doi.org/10.1016/S0378-8741(99)00011-2
  7. Lee, Y.Y., Hsu, F.L., Nohara, T. Two new soladulcidine glycosides from Solanum lyratum. Chemical Pharmaceutical Bullutin 45: 1381-1382, 1997 https://doi.org/10.1248/cpb.45.1381
  8. Kang, B., Lee, E., Hong, I., Lee, J., Kin, H. Abolition of anaphylactic shock by Solanum lyratum Thunb. Int. J. Immunopharmacol. 19: 729, 1997 https://doi.org/10.1016/S0192-0561(97)00035-0
  9. Sun, L.X., Fu, W.W., Ren, J., Xu, L., Bi, K.S., Wanq, M.W. Cytotoxic constituents from Solanum lyratum. Arch Pharm Res. 29: 135-139, 2006 https://doi.org/10.1007/BF02974274
  10. Sun, L.X., Fu, W.W., Li, W., Bi, K.S., Wanq, M.W. Diosgenin glucuronides from Solanum lyratum and their cytotoxicity against tumor cell lines.. Z Naturforsch 61(3-4):171-176, 2006
  11. Kremer, J.M., Westhovens, R., Leon, M., Di Giorgio, E., Alten, R., Steinfeld, S. et al.. Treatment of rheumatoid arthritis by selective inhibition of T-cell activation with fusion protein CTLA4 Ig. The New England Journal of Medicine 349: 1907-1915, 2003 https://doi.org/10.1056/NEJMoa035075
  12. Luross, J.A., Williams, N.A. The genetic and immunopathological processes underlying collagen-induced arthritis. Immunology 103: 407-416, 2001 https://doi.org/10.1046/j.1365-2567.2001.01267.x
  13. Wooley, P.H., Chapedelaine, J.M. Immunogenetics of collagen-induced arthritis. Critical Reviews in Immunology 8: 1-22, 1987
  14. Janossy, G., Duke, O., Poulter, L.W., Panayi G., Bofill M. and Goldstein G. rheumatoid arthritis : a disease of t-lymphocyte/macrophage immunoregulation Lancet 318: 839, 1981 https://doi.org/10.1016/S0140-6736(81)91107-7
  15. Quayle, A.J., Chomarat, P., Miossec, P., Kjeldsen-Kragh, J., Forre, O. and Natvig, J.B. Rheumatoid inflammatory T-cell clones express mostly Th1 but also Th2 and mixed (Th0-like) cytokine patterns. Scandinavian Journal of Immunology. 38: 75-82, 1993
  16. Dolhan, R.J. E.M., van der Heiden, A.N., ter Haar, N.T., Breedveld, F.C. and Miltenburg, A.M.M. Shift toward T lymphocytes with a T helper 1 cytokine-secretion profile in the joints of patients with rheumatoid arthritis. ARTHRITIS & RHEUMATISM. 39: 1961-1969, 1996 https://doi.org/10.1002/art.1780391204
  17. Kadowaki, K.M., Matsuno, H., Tsuji, H., Tunru, I. CD4+ T cells from collagen-induced arthritic mice are essential to transfer arthritis into severe combined immunodeficient mice. Clinical andExperimental Immunology 97: 212-218, 1994
  18. Williams, R.O., Whyte, A., Waldmann, H. Resistance to collageninduced arthritis in DBA/1 mice by intraperitoneal administration of soluble type II collagen involves both CD4+ and CD8+ T lymphocytes. Autoimmunity 4: 237-245, 1989 https://doi.org/10.3109/08916938909014700
  19. Takemura, S., Klimiuk, P.A., Braun, A. et al. T cell activation in rheumatoid synovium is B cell dependent. Journal of Immunology 167: 4710-4718, 2001 https://doi.org/10.4049/jimmunol.167.8.4710
  20. Paolo Serafini, Carmela De Santo, Ilaria Marigo, Sara Cingarlini, Luigi Dolcetti, Giovanna Gallina, Paola Zanovello, Vincenzo Bronte. Derangement of immune responses by myeloid suppressor cells. Cancer Immunol Immunother 53: 6472, 2004
  21. Jean-Michel Dayer. The process of identifying and understanding cytokines: from basic studies to treating rheumatic diseases. Best Practice & Research Clinical Rheumatology 18(1):3145, 2004