Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Thrombin Inhibition Activity of Fructus Extract of Crataggus pinnatifida Bunge

산사자 추출물의 트롬빈 저해활성

  • Ryu, Hee-Young (Dept. of Food and Nutrition, Andong National University) ;
  • Kim, Yung-Kwan (Dept. of Food and Nutrition, Andong National University) ;
  • Kwun, In-Sook (Dept. of Food and Nutrition, Andong National University) ;
  • Kwon, Chong-Suk (Dept. of Food and Nutrition, Andong National University) ;
  • Jin, Ing-Nyol (Dept. of Microbiology, School of Life Science and Biotechnology Kyungpook National University) ;
  • Sohn, Ho-Yong (Dept. of Food and Nutrition, Andong National University)
  • 류희영 (안동대학교 식품영양학과) ;
  • 김영관 (안동대학교 식품영양학과) ;
  • 권인숙 (안동대학교 식품영양학과) ;
  • 권정숙 (안동대학교 식품영양학과) ;
  • 진익렬 (경북대학교 미생물학과) ;
  • 손호용 (안동대학교 식품영양학과)
  • Published : 2007.04.25
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Abstract

The fructus of Crataegus pinnatifida Bunge has been used as medicinal and food source in worldwide. In this study, a strong direct thrombin inhibition and antithrombosis activity were identified from the methanol extract of C. pinnatifida Bunge fructus. The solvent fractionation of fructus extract using hexane, ethylacetate, butanol revealed that the butanol fraction has a prominent antithrombin activity. Thrombin time(blood-clot formation time) and activated partial thromboplastin time(aPTT) extended to 835% and 315% by addition of the butanol fraction at concentration of 1.25 mg/mL, whereas thrombin time extended to 287% by addition of aspirin at concentration of 1,25 mg/mL. The butanol fraction showed anthrone-positive and weak ninhydrine-postive reaction. The thrombin inhibitory activity was not related to previously reported flavonoids or polyphenols. The activity was maintained against acid treatment(0.5 N HCl for 120 min), but rapidly lost by heat-treatment($100^{\circ}C$ for 30 min). Our results suggested that fructus of C. pinnatifida Bunge with non-heat treatment process could be developed as a natural source of antithrombosis.

식용 및 약용으로 광범위하게 이용되고 있는 산사자의 유용 생리활성 검토를 목적으로 산사자 추출물 및 분획물을 조제하여, 혈액내의 혈전 생성에 직접 관여하는 인간 트롬빈의 thrombin time(TT) 및 activated partial thromboplastin time(aPTT) 증가 활성을 측정하여 항혈전 활성을 평가하였다. 산사자의 항혈전 물질은 물 추출보다는 메탄을 추출이 적합하며(추출효율 40.4%), butanol 분획에서 가장 강력한 활성을 나타내었다. 산사자의 butanol 분획물은 1.25 mg/ml의 농도에서 835%의 TT 증가, 315%의 aPTT 증가를 보여 아스피린보다 2.9배 이상의 강력한 항혈전 활성을 나타내었다. 이러한 항혈전 활성은 기존의 보고된 quercetin, kaempferol, myricetin 및 rutin과는 무관하였다. 또한 산사자의 활성물질은 0.5 N HCl 처리시 120분 이상 안정하였으나, $100^{\circ}C$, 30분 열처리에는 85% 이상의 활성소실이 나타나 열에 매우 민감한 물질로 확인되었다. 본 연구결과는, 비열 처리와 적합한 가공 공정이 가능하다면 산사자 추출물이 혈액순환 장해 및 혈관계 질환 예방 및 치료제로 개발 가능함을 제시하고 있다.

Keywords

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