Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

A Cytoplasmic Polyhedrosis Virus Isolated from the Pine Processionary Caterpillar, Thaumetopoea pityocampa

  • Ince, Ikbal Agah (Department of Biology, Faculty of Arts and Sciences, Karadeniz Technical University) ;
  • Demir, Ismail (Department of Biology, Faculty of Arts and Sciences, Karadeniz Technical University) ;
  • Demirbag, Zihni (Department of Biology, Faculty of Arts and Sciences, Karadeniz Technical University) ;
  • Nalcacioglu, Remziye (Department of Biology, Faculty of Arts and Sciences, Karadeniz Technical University)
  • Published : 2007.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

A cytoplasmic polyhedrosis virus (CPV) was isolated from the larvae of Thaumetopoea pityocampa and shown to cause an infection of midgut cells. This viral infection revealed several important diagnostic symptoms, including discoloration of the posterior midgut, reduced feeding, and extended development time of the larvae. The virus infection is lethal to Thaumetopoea pityocampa, and with the increasing doses kills the larvae within 4-5 days post infection. Electron microscopy studies showed typical cytoplasmic polyhedral inclusion bodies that are icosahedral, and ranged from 2.4 to $5.3{\mu}m$ in diameter. Electrophoretic analysis of the RNA genome showed that the virus has a genome composed of 10 equimolar RNA segments with the sizes of 3,907, 3,716, 3,628, 3,249, 2,726, 1,914, 1,815, 1,256, 1,058, and 899 bp, respectively. Based on morphology and nucleic acid analysis, this virus was named Thaumetopoea pityocampa cytoplasmic polyhedrosis virus (TpCPV), and belongs to the genus Cypovirus, family Reoviridae.

Keywords

References

  1. Abbott, W. S. 1925. A method of computing the effectiveness of an insecticide. J. Econ. Entomol. 18: 265-267
  2. Belloncik, S. 1989. Cytoplasmic polyhedrosis viruses - Reoviridae. Adv. Vir. Res. 37: 173-209 https://doi.org/10.1016/S0065-3527(08)60835-0
  3. Belloncik, S. and H. Mori. 1998. Cypoviruses, pp. 337-369. In L. K. Miller and L. B. Andrew (eds.), The Insect Viruses. Plenum Press, New York and London
  4. Biliotti, E. 1959. Oservations epizootiologiques sur la processionnare du pin. Rev. Pathol. Veget. d'Entomol. Agric. France 38: 149-155
  5. Ducombs, G., M. Lamy, S. Mollard, J. M. Guillard, and J. Maleville. 1981. Contact dermatitis from processionary caterpillar (Thaumetopoea pityocampa Schiff Lepidoptera). Contact Dermatitis 7: 287 https://doi.org/10.1111/j.1600-0536.1981.tb04081.x
  6. Foillaud, M. and G. Morel. 1994. Characterisation of cytoplasmic and nuclear polyhedrosis viruses recovered from the nest of Polistes hebraeus F. (Hymenoptera; Vespidae). J. Inverteb. Pathol. 64: 89-95 https://doi.org/10.1006/jipa.1994.1075
  7. Fujii-Kawata, I., K. Miura, and M. Fuke. 1970. Segments of genome of viruses containing double stranded ribonucleic acid. J. Mol. Biol. 51: 247-253 https://doi.org/10.1016/0022-2836(70)90140-3
  8. Granados, R. R. and K. A. Williams. 1986. In vivo infection and replication of baculoviruses, pp. 89-108. In R. R. Granados and B. A. Federici (eds.), The Biology of Baculoviruses. Vol. I. Biological Properties and Molecular Biology. CRC Press, Boca Raton
  9. Green, T. B., A. Shapiro, S. White, S. Rao, P. P. C. Mertens, G. Carner, and J. J. Becnel. 2006. Molecular and biological characterization of a Cypovirus from the mosquito Culex restuans. J. Inverteb. Pathol. 91: 27-34 https://doi.org/10.1016/j.jip.2005.10.007
  10. Grison, P. 1959. Reflexiones sobre la utilization de Smithiavirus pytiocampa Schiff. Boln. Serv. Plagas For. Madrid 12: 105-112
  11. Grison, P. and C. Vago. 1959. La lutte contre la processionnarie du pin 'Thaumetopoea pityocampa' Schiff dans le massif du ventoux. Essai d'utilisation pratique d'un virus specifique. Rev. Fores. France 353-370
  12. Hagiwara, K., M. Tomita, K. Nakai, J. Kobayashi, S. Miyajima, and T. Yoshimura. 1998. Determination of the nucleotide sequence of Bombyx mori cytoplasmic polyhedrosis virus segment 9 and its expression in BmN4 cells. J. Virol. 72: 5762-5768
  13. Hukuhara, T. and J. R. Bohami. 1991. Reoviridae, pp. 394-430. In J. R. Adams and J. R. Bohami (eds.), Atlas of Invertebrates. CRC Press, Boca Raton
  14. Kanat, M. and M. H. Alma. 2003. Insecticidal effects of essential oils from various plants against larvae of pine processionary moth (Thaumetopoea pityocampa Schiff) (Lepidoptera: Thaumetopoeidae). Pest Manag. Sci. 60: 173-177 https://doi.org/10.1002/ps.802
  15. Martouret, D. and G. Dusaussoy. 1959. Multiplication et extraction des corps d'inclusion de la virose intestinale de Thaumetopoea pityocampa Schiff. Entomophaga 4: 253-259 https://doi.org/10.1007/BF02374881
  16. Mertens, P. P. C., N. E. Crook, R. Rubinstein, S. Pedley, C. C. Payne. 1989. Cytoplasmic polyhedrosis virus classification by electropherotype; validation by serological analyses and agarose gel electrophoresis. J. Gen. Virol. 70: 173-185 https://doi.org/10.1099/0022-1317-70-1-173
  17. Mertens, P. P. C., S. Pedley, N. E. Crook, R. Rubinstein, and C. C. Payne. 1999. A comparison of six cypovirus isolates by cross-hybridisation of their dsRNA genome segments. Arch. Virol. 144: 561-576 https://doi.org/10.1007/s007050050525
  18. Mertens, P. P. C., S. Rao, and H. Zhou. 2005. Cypovirus, pp. 522-533. In C. M. Fauquet, M. A. Mayo, J. Maniloff, U. Desselberger, and L. A. Ball (eds.), Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses. Elsevier/Academic Press, London, England
  19. Park, S.-W, D.-H. Chung, B.-Y. Ahn, and P.-W Lee. 2006. Generation and segregation of hantaviral RNA genomic diploid; implications of reassortant generation mechanism. J. Microbiol. Biotechnol. 16: 1017-1025
  20. Payne, C. C. 1981. Cytoplasmic polyhedrosis viruses, pp. 61-100. In E. W. Davidson, N. J. Totowa, and A. Omsun (eds.), Pathogenesis of Invertebrate Microbial Diseases
  21. Payne, C. C. and C. F. Rivers. 1976. A provisional classification of cytoplasmic polyhedrosis viruses based on the sizes of the RNA genome segments. J. Gen. Virol. 33: 71-85 https://doi.org/10.1099/0022-1317-33-1-71
  22. Payne, C. C. and P. P. C. Mertens. 1983. The cytoplasmic polyhedrosis viruses, pp. 425-503. In W. K. Joklik (ed.), The Reoviridae. Plenum, New York
  23. Shapiro, A., T. Green, S. Rao, S. White, G. Cartner, P. P. C. Mertens, and J. Becnel. 2005. Morphological and molecular characterization of a cypovirus from the mosquito Uranotaenia sapphirina (Diptera: Culicidae). J. Virol. 79: 9430-9438 https://doi.org/10.1128/JVI.79.15.9430-9438.2005
  24. Sidor, C., B. Zamola, A. Zega, and L. Dusanic. 1982. A disease of pine processionary (Thaumetopoea pityocampa) caused by a nuclear-type polyhedral virus. Redia 65: 89-96
  25. Tsankov, G., L. Encheva, and P. Kaitazova. 1979. A virus disease of larvae of the pine processionary moth, Thaumetopoea pityocampa. Gorskostopanska Nauka 16: 68-71
  26. Xia, D., Y. K. Sun, M. A. McCrae, and M. G. Rossmann. 1991. X-ray powder pattern analysis of cytoplasmic polyhedrosis virus inclusion bodies. Virology 180: 153-158 https://doi.org/10.1016/0042-6822(91)90018-7
  27. Vago, C. 1953. La polyedrie de Thaumetopoea pityocampa. Ann. Epiphy. 319-332
  28. Vago, C. 1958. Virose intestinale chez la processionnarie du pin Thaumetopoea pityocampa Schiff (Lepidoptera). Entomophaga 3: 35-37 https://doi.org/10.1007/BF02372197
  29. Vago, C. 1959. Sur le mode d'infection de la virose intestinale de Thaumetopoea pytiocampa Schiff. Entomophaga 4: 311-414 https://doi.org/10.1007/BF02373367
  30. van Regenmortel, M. H. V., C. M. Fauquet, D. H. L. Bishop, E. B. Carstens, M. K. Estes, S. M. Lemon, J. Maniloff, M. A. Mayo, D. J. McGeoch, C. R. Pringle, and R. B. Wickner (eds.). 2000. pp. 445-455. Virus Taxonomy. Classification and Nomenclature of Viruses. Seventh Report of the International Committee on Taxonomy of Viruses. Academic Press, San Diego, U.S.A
  31. Vega, J. M., I. Moneo, A. Armentia, A. Fernandez, J. Vega, J. R. De La Fuente, P. Sanchez, and M. E. Sanchis. 1999. Allergy to the pine processionary caterpillar (Thaumetopoea pityocampa). Clin. Exp. Aller. 29: 1418-1423 https://doi.org/10.1046/j.1365-2222.1999.00626.x
  32. Vega, J. M., I. Moneo, A. Armentia, R. Lopez-Rico, G. Curiel, B. Bartolome, and A. Fernandez. 1997. Anaphylaxis to a pine caterpillar. Allergy 52: 1244-1245 https://doi.org/10.1111/j.1398-9995.1997.tb02532.x
  33. Werno, J. and M. Lamy. 1990. Pollution atmospherique d'originale animale: les poils urticans de la chenille processionnaire du pin (Thaumetopoea pityocampa Schiff., Insectes, Lepidopteres). C. R. Acad. Sci. Paris 310: 325-331
  34. Werno J., M. Lamy, and P. Vincendeau. 1993. Caterpillars hairs as allergens. Lancet 342: 936-937
  35. Wigley, P. J. 1980. Practical: Diagnosis of virus infections - staining of insect inclusion body viruses, pp. 35. In J. Kalmakoff and J. F. Longworth (eds.), Microbial Control cf Insect Pests. New Zealand Department of Science and Industrial Research, Bulletin 228. Wellington, New Zealand
  36. Zeddam, J. L., J. A. Cruzado, J. L. Rodriguez, M. Ravallec, and E. C. Subilette. 2003. A cypovirus from the South American oil palm pest Norape argyrrhorea and its potential as a microbial control agent. Biocontrol 48: 101-112 https://doi.org/10.1023/A:1021234700472