Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Inhibition of Seed Germination and Induction of Systemic Disease Resistance by Pseudomonas chlororaphis O6 Requires Phenazine Production Regulated by the Global Regulator, GacS

  • Kang, Beom-Ryong (Agricultural Plant Stress Research Center and Environmental-Friendly Agricultural Research Center, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Han, Song-Hee (Agricultural Plant Stress Research Center and Environmental-Friendly Agricultural Research Center, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Zdor, Rob E. (Department of Biology Andrews University) ;
  • Anderson, Anne J. (Department of Biology, Utah State University) ;
  • Spencer, Matt (Department of Biology, Utah State University) ;
  • Yang, Kwang-Yeol (Agricultural Plant Stress Research Center and Environmental-Friendly Agricultural Research Center, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Kim, Yong-Hwan (National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Lee, Myung-Chul (National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Cho, Baik-Ho (Agricultural Plant Stress Research Center and Environmental-Friendly Agricultural Research Center, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Kim, Young-Cheol (Agricultural Plant Stress Research Center and Environmental-Friendly Agricultural Research Center, College of Agriculture and Life Sciences, Chonnam National University)
  • Published : 2007.04.30
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Abstract

Seed coating by a phenazine-producing bacterium, Pseudomonas chlororaphis O6, induced dose-dependent inhibition of germination in wheat and barley seeds, but did not inhibit germination of rice or cucumber seeds. In wheat seedlings grown from inoculated seeds, phenazine production levels near the seed were higher than in the roots. Deletion of the gacS gene reduced transcription from the genes required for phenazine synthesis, the regulatory phzI gene and the biosynthetic phzA gene. The inhibition of seed germination and the induction of systemic disease resistance against a bacterial soft-rot pathogen, Erwinia carotovora subsp. carotovora, were impaired in the gacS and phzA mutants of P chlororaphis O6. Culture filtrates of the gacS and phzA mutants of P. chlororaphis O6 did not inhibit seed germination of wheat, whereas that of the wild-type was inhibitory. Our results showed that the production of phenazines by P. chlororaphis O6 was correlated with reduced germination of barley and wheat seeds, and the level of systemic resistance in tobacco against E. carotovora.

Keywords

References

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