Effects of Follicle Stimulating Hormone and Human Chorionic Gonadotrophin on the In Vitro Maturation of Canine Oocytes

  • Kim, Min-Kyu (Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University) ;
  • Oh, Hyun-Ju (Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University) ;
  • Jang, Goo (Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University) ;
  • Hong, So-Gun (Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University) ;
  • Park, Jung-Eun (Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University) ;
  • Kim, Hye-Jin (Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University) ;
  • Lee, Hyung-Suk (Woosong Information College) ;
  • Kim, Sang-Cheol (Department of Animal Science, Shingu College) ;
  • Kang, Sung-Keun (Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University) ;
  • Lee, Byeong-Chun (Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University)
  • 발행 : 2007.03.31

초록

The present study investigated the effects of follicle stimulating hormone (FSH) and human chorionic gona-dotrophin (hCG) on the nuclear maturation of canine oocytes. Oocytes were recovered from mongrel female ovaries in various reproductive states; follicular, luteal or anestrous stage. Oocytes were cultured in sserum-free tissue culture medium (TCM)-199 supplemented with various concentrations of FSH (Exp. 1: 0, 0.5, 1.0 or 10 IU) or hCG (Exp.2:0, 0.5, 1.0 or 10 IU) or both (Exp. 3:1 IU FSH +1 IU hCG) for 72 hr to determine the effective concentration of these hormones, and to examine their combined effect. After maturation culture, oocytes were denuded in PBS containing 0.1% (w/v) hyaluronidase by gentle pipetting. The denuded oocytes were stained with $1.9\;{\mu}M$. Hoechst 33342 in glycerol and the nuclear state of oocytes was evaluated under UV light. More (p<0.05) oocytes matured to MII stage when follicular stage oocytes were supplemented with 1 IU FSH (6.2%) compared with the control, 0.1 or 10.0 IU FSH (0 to 1.2%). Significantly higher (p<0.05) maturation rate to MII stage was observed in follicular stage oocytes supplemented with 1.0 IU hCG (7.2%) compared with the control or other hCG supplemented groups (0 to 1.5%). However, the combination of FSH and hCG did not improve the nuclear maturation rate of canine oocyte (2.4 %) compared with FSH (6.2%) and hCG alone (7.2%). In conclusion, FSH or hCG alone significantly increased the maturation of canine oocytes to MII stage.

키워드

참고문헌

  1. Abir R, Franks S, Mobberley MA, Moore PA, Margara RA, Winston RM (1997): Mechanical isolation and in vitro growth of preantral and small antral human follicles. Fertil Steril 68:682-688 https://doi.org/10.1016/S0015-0282(97)00264-1
  2. Albertini DF (1992): Cytoplasmic microtubular dynamics and chromatin organization during mammalian oogenesis and oocyte maturation. Mutat Res 296:57- 68 https://doi.org/10.1016/0165-1110(92)90032-5
  3. Albertini DF, Anderson E (1974): The appearance and structure of intercellular connections during the ontogeny of the rabbit ovarian follicle with particular reference to gap junctions. J Cell BioI 63: 234-250 https://doi.org/10.1083/jcb.63.1.234
  4. Anderson E, Albertini DF (1976): Gap junctions between the oocyte and companion follicle cells in the mammalian ovary. J Cell BioI 71:680-686 https://doi.org/10.1083/jcb.71.2.680
  5. Baker SJ, Spears N (1997): Follicle stimulating hormone inhibits apoptosis in pre- and early-antral murine follicles in vitro. J Reprod Fertil 19:21
  6. Camp TA, Rahal JO, Mayo KE (1991): Cellular localization and hormonal regulation of follicle-stimulating hormone and luteinizing hormone receptor messenger RNAs in the rat ovary. Mol. Endocrinol 5:1405-1417 https://doi.org/10.1210/mend-5-10-1405
  7. Chun SY, Billig H, Tilly JL, Furuta I, Tsafriri A, Hsueh AJ (1994): Gonadotropin suppression of apoptosis in cultured preovulatory follicles: mediatory role of endogenous insulin-like growth factor I. Endocrinology 135:1845-1853 https://doi.org/10.1210/en.135.5.1845
  8. Chun SY, Eisenhauer KM, Minami S, Billig H, Perlas E, Hsueh AJ (1996): Hormonal regulation of . apoptosis in early antral follicles: follicle stimulating hormone as a major survival factor. Endocrinology 137:1447-1456 https://doi.org/10.1210/en.137.4.1447
  9. Concannon PW, McCann JP, Temple M (1989): Biology and endocrinology of ovulation, pregnancy and parturition in the dog. J Reprod Fertil (Suppl) 39:3-25
  10. Concannon PW (1993): Biology of gonadotrophin secretion in adult and prepubertal female dogs. J Reprod Fertil (Suppl) 47:23-27
  11. Cortvrindt R, Smitz J, Van Steirteghem AC (1997): Assessment of the need for follicle stimulating hormone in early preantral mouse follicle culture in vitro. Hum Reprod 12:759-768 https://doi.org/10.1093/humrep/12.4.759
  12. Dekel N, Beers WH (1978): Rat oocyte maturation in vitro. relief of cyclic AMP inhibition by gonadotropins. Proc Nat! Acad Sci USA 75:4369-4373
  13. Dierich A, Sairam MR, Monaco L, Fimia GM, Gansmuller A, LeMeur M, Sassone-Corsi P (1998): Impairing follicle-stimulating hormone (FSH) signaling in vivo. targeted disruption of the FSH receptor leads to aberrant gametogenesis and hormonal imbalance. Proc Natl Acad Sci USA 95:13612-13617
  14. Downs SM, Daniel SA, Eppig JJ (1988): Induction of maturation in cumulus cell-enclosed mouse oocytes by follicle-stimulating hormone and epidermal growth factor: evidence for a positive stimulus of somatic cell origin. J Exp Zool 245:86-96 https://doi.org/10.1002/jez.1402450113
  15. Edwards RG (1965): Maturation in vitro of human ovarian oocytes. Lancet 7419:926-929
  16. Hewitt DA, England GC (1999): Synthetic oviductal fluid and oviductal cell coculture for canine oocyte maturation in vitro. Anim Reprod Sci 55:63-75 https://doi.org/10.1016/S0378-4320(98)00162-6
  17. Hillier SG. (2001): Gonadotropic control of ovarian follicular growth and development. Mol Cell Endocrinol 179:39-46 https://doi.org/10.1016/S0303-7207(01)00469-5
  18. Jeffcoat I (1998): Physiology and endocrinology of reproduction in female dogs. 1st ed. (Simpson GM, England GCW, Harvey M (eds), BSAVA manual of Small Animal Reproduction and Neonatology, British Small Animal Veterinary Association, Shurdington, pp. 1-9
  19. Kim MK, Fibrianto YH, Oh HJ, Jang G, Kim HJ, Lee KS, Kang SK, Lee BC, Hwang WS (2004): Effect of beta-mercaptoethanol or epidermal growth factor supplementation on in vitro maturation of canine oocytes collected from dogs with different stages of the estrus cycle. J Vet Sci 3:253-258
  20. Luvoni GC, Luciano AM, Modina S, Gandolfi F (2001) Influence of different stages of the oestrous cycle on cumulus-oocyte communications in canine oocytes: effects on the efficiency of in vitro maturation. J Reprod Fertil (Suppl) 57:141-146
  21. Mattioli M, Bacci M.L, Galeati G, Seren E (1991): Effects of LH and FSH on the maturation of pig oocytes in vitro. Theriogenology 36:95-105 https://doi.org/10.1016/0093-691X(91)90438-J
  22. McGee EA, Perlas E, LaPolt PS, Tsafriri A, Hsueh AJ (1997): Follicle-stimulating hormone enhances the development of preantral follicles in juvenile rats. BioI Reprod 57:990-998 https://doi.org/10.1095/biolreprod57.5.990
  23. Nayudu PL, Osborn SM (1992): Factors influencing the rate of preantral and antral growth of mouse ovarian follicles in vitro. J Reprod Fertil 95:349-362 https://doi.org/10.1530/jrf.0.0950349
  24. O'Shaughnessy PJ, Dudley K, Rajapaksha WR (1996): Expression of follicle stimulating hormonereceptor mRNA during gonadal development. Mol Cell Endocrinol 125:169-175 https://doi.org/10.1016/S0303-7207(96)03957-3
  25. Pincus G, Enzmann EV (1934): Can mammalian eggs undergo normal development in vitro. Proc Natl Acad Sci USA 20:121-122
  26. Roy SK, Treacy BJ (1993): Isolation and long-term culture of human preantral follicles. Fertil Steril 59: 783-790 https://doi.org/10.1016/S0015-0282(16)55860-9
  27. Singh B, Meng L, Rutledge JM, Armstrong DT (1997): Effects of epidermal growth factor and follicle-stimulating hormone during in vitro maturation on cytoplasmic maturation of porcine oocytes. Mol Reprod Dev 46:401-407 https://doi.org/10.1002/(SICI)1098-2795(199703)46:3<401::AID-MRD20>3.0.CO;2-#
  28. Songsasen N, Yu I, Leiba SP (2002): Nuclear maturation of canine oocytes cultured in protein-free media. Mol Reprod Dev 62:407-415 https://doi.org/10.1002/mrd.10130
  29. Spears N, Boland NI, Murray AA, Gosden RG (1994) Mouse oocytes derived from in vitro grown primary ovarian follicles are fertile. Hum Reprod 9:527-532 https://doi.org/10.1093/oxfordjournals.humrep.a138539
  30. Themmen APN, Huhtaniemi IT (2000): Mutations of gonadotropins and gonadotropin receptors: elucidating the physiology and pathophysiology of pitui- tary-gonadal function. Endocr Rev 21:551-583 https://doi.org/10.1210/er.21.5.551
  31. Tilly JL, Tilly KI (1995): Inhibitors of oxidative stress mimic the ability of follicle-stimulating hormone to suppress apoptosis in cultured rat ovarian follicles. Endocrinology 136:242-252 https://doi.org/10.1210/en.136.1.242
  32. Wen XH, Feng HL, Sun QY (1994): In vitro maturation of follicular oocytes of the silver fox (Vulpes fulva desm). Theriogenology 41:333 https://doi.org/10.1016/S0093-691X(05)80243-2
  33. Yamada S, Shimazu Y, Kawano Y, Nakazawa M, Naito K, Toyoda Y (1993): In vitro maturation and fertilization of preovulatory dog oocytes. J Reprod Fertil (Suppl) 47:227-229
  34. Zuelke KA, Brackette BG (1990): Luteinizing hormone-enhanced in vitro maturation of bovine oocytes with and without protein supplementation. BioI Reprod 43:784-787 https://doi.org/10.1095/biolreprod43.5.784
  35. Zuelke KA, Brackette BG (1993): Increased glutamine metabolism in bovine cumulus cell-enclosed and denuded oocytes after in vitro maturation with luteinizing hormone. Biol Reprod 48:815-820 https://doi.org/10.1095/biolreprod48.4.815