Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Risk Assessment of Nonylphenol using Sex Ratio, Sexual Maturation, Intersex and Lipofuscin Accumulation of the Equilateral Venus Gomphina veneriformis (Bivalvia: Veneridae)

대복 Gomphina veneriformis의 성비, 성 성숙, intersex 및 지방갈색소 침적을 이용한 nonylphenol의 위해성 평가

  • Lee, Jung-Sick (Department of Aqualife Medicine, College of Fisheries and Ocean Science Chonnam National University) ;
  • Park, Jung-Jun (Department of Aqualife Medicine, College of Fisheries and Ocean Science Chonnam National University)
  • 이정식 (전남대학교 수산해양대학 수산생명의학과) ;
  • 박정준 (전남대학교 수산해양대학 수산생명의학과)
  • Published : 2007.02.28
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Abstract

Nonylphenol (NP) is an estrogen-mimicking compound or xenoestrogen. This study investigated the effects of nonylphenol on the reproductive status of the equilateral venus Gomphina veneriformis. The experiment lasted 24 weeks, Experimental groups consisted of a control and three nonylphenol exposures ($1.0,\;2.5,\;and\;5.0\;{\mu}g\;NP/L$). Mortality did not differ significantly between the control and the exposure groups. The sex ratio (F:M) was 1:1 in nature and 1:1.03 in the control group. However, it changed to 1:3.5 with $5.0\;{\mu}g\;NP/L$ exposure. Gonad maturity in females was higher in the nonylphenol exposure groups than in the control group. By contrast, in males, it was lower in the nonylphenol exposure groups. Intersex individuals constituted 0% in nature, 3.08% in the control group, and 23.6% in the group exposed to nonylphenol, with female characteristics more prevalent than male. As the concentration of nonylphenol increased, the accumulation of lipofuscin increased in the mid-gut gland.

Keywords

References

  1. Ackermann, G.E., J. Schwaiger, R.D. Negele and K. Fent. 2002. Effects of long-term nonylphenol exposure on gonadal development and biomarkers of estrogenicity in juvenile rainbow trout, Oncorhynchus mykiss. Aquat. Toxicol., 60, 203-221 https://doi.org/10.1016/S0166-445X(02)00003-6
  2. Barbeau, C.R. and et J.-E. Cote Bougie, 1981. Temporal and spatial variations of mercury, lead, zinc, and copper in sediments of the Saguenay Fjord. Can. J. Earth Sci., 18, 1065-1074 https://doi.org/10.1139/e81-102
  3. Blaise, C., J. Pellerin-Massicote and P.D. Hansen. 1996. Molluscan shellfish biomarker study of the Saguenay fjord, Quebec, Canada. Fourth European Conference on Ecotoxicology and Environmental Safety, Metz., France, 25-28 August, Abstract C41
  4. Bortone, S.A. and W.P. Davis. 1994. Fish intersexuality as indicator of environmental stress: Monitoring fish reproductive systems can serve to alert humans to potential harm. BioSci., 44, 165-172 https://doi.org/10.2307/1312253
  5. Byrne, P.A. and J. O'Halloran. 1999. Aspects of assaying sediment toxicity in Irish estuarine ecosystems. Mar. Pollut. Bull., 39, 97-105 https://doi.org/10.1016/S0025-326X(99)00121-6
  6. Byrne, P.A. and J. O'Halloran. 2001. The role of bivalve molluscs as tools in estuarine sediment toxicity testing: a review. Hydrobiologia, 465, 209-217 https://doi.org/10.1023/A:1014584607501
  7. Drysdale, D.T. and S.A. Bortone. 1989. Laboratory induction of inter-sexuality in the mosquitofish, Gambusia affinis, using paper mill effluent. Bull. Environ. Contam. Toxicol., 43, 611-617 https://doi.org/10.1007/BF01701943
  8. Eversole, A.G. 1997. Gametogenesis of Mercenaria mercenaria, M. campechiensis and their hybrids. Nautilus, 110, 107-110
  9. Gagne, F., C. Blaise, J. Pellerin, E. Pelletier, M. Douville, S. Gauthier-Clerc and L. Viglino. 2003. Sex alteration in soft-shell clams (Mya arenaria) in an intertidal zone of the Saint Lawrence River (Quebec, Canada). Comp. Biochem. Physiol. C, 134, 189-198 https://doi.org/10.1016/S1096-4959(02)00250-6
  10. Gauthier-Clerc, S., J. Pellerin, C. Blaise and F. Gagne. 2002. Delayed gametogenesis of Mya arenaria in the Saguenay Fjord (Canada): a consequence of endocrine disruptors? Comp. Biochem. Physiol. C, 131, 457-467 https://doi.org/10.1016/S1095-6433(01)00485-8
  11. Gibbs, P.E. and P.A. Byrne. 1986. Reproductive failure in populations of the dog-whelk, Nucella lapillus, caused by imposex induced by tributyltin from antifouling paints. J. Mar. Biol. Ass. U.K., 66, 767-777 https://doi.org/10.1017/S0025315400048414
  12. Gibbs, P.E., P.L. Pascoe and G.R. Burt. 1988. Sex change in the female dog-whelk Nucella lapillus induced by tributyltin from antifouling paints. J. Mar. Biol. Ass. U.K., 68, 715-731 https://doi.org/10.1017/S0025315400028824
  13. Gimeno, S., H. Komen, S. Jobling, J. Sumpter and T. Bowmer. 1998. Demasculinisation of sexually mature male common carp, Cyprinus carpio, exposed to 4-tert-pentylphenol during spermatogenesis. Aquat. Toxicol., 43, 93-109 https://doi.org/10.1016/S0166-445X(98)00060-5
  14. Gray, M.A. and C.D. Metcalfe. 1997. Induction of testisova in Japanese medaka Oryzias latipes exposed to p-nonyiphenoi. Environ. Toxicol. Chem., 16, 1082-1086 https://doi.org/10.1897/1551-5028(1997)016<1082:IOTOIJ>2.3.CO;2
  15. Greak, B.F. and P.L. Layman. 1989. Higher costs spur new detergent formulations. Chem. Eng. News, 23, 29-49
  16. Hole, L.M., M.N. Moore and D. Bellamy. 1995. Agerelated cellular and physiological reactions to hypoxia and hyperthermia in marine mussels. Mar. Ecol. Prog. Ser., 122, 173-178 https://doi.org/10.3354/meps122173
  17. Horiguchi, T., H. Shiraishi, M. Shimizu, and M. Morita. 1994. Imposex and organotin compounds in Thais clavigera and T. bronni in Japan. J. Mar. Biol. Ass. U.K., 74, 651-669 https://doi.org/10.1017/S002531540004772X
  18. Horiguchi, T., N. Takiguchi, H.S. Cho, M. Kojima, M. Kaya, H. Shiraishi, M. Morita, H. Hirose, and M. Shimizu. 2000. Ovo-testis and disturbed reproductive cycle in the giant abalone Haliotis madaka: possible linkage with organotin contamination in a site of population decline. Mar. Environ. Res., 50, 223-229 https://doi.org/10.1016/S0141-1136(00)00071-4
  19. Ju, S.M. 2006. Biological response of the equilateral venus, Gomphina veneriformis (Bivalvia: Veneridae) with zinc bioaccumulation. MS Thesis, Yosu Nat'l Univ., Yeosu, Korea, pp. 50
  20. Kahng, S.H., J.G. Je, J.R. Oh, W.J Shim and J.H. Shim. 1996. Imposex of Thais clavigera and T. luteostoma (Muricidae) as an evidence of organotin pollution in Chinhae Bay. Kor. J. Malacol., 12, 123-131
  21. Khim, J.S., D.L. Villeneuve, K. Kannan, K.T. Lee, S.A. Snyder, C.H. Koh and J.P. Giesy. 1999. Characterization and distribution of trace organic contaminants in sediment from Masan Bay, Korea. Inst. Analy. Environ. Sci. Technol., 33, 4199-4205
  22. Krishnakumar, P.K., E. Casillas and U. Varanasi. 1994. Effect of environmental contaminants on the health of Mytilus edulis from Puget Sound, Washington, USA: I. Cytochemical measures of lysosomal responses in the digestive cells using automatic image analysis. Mar. Ecol. Prog. Ser., 106, 249-261 https://doi.org/10.3354/meps106249
  23. Krishnakumar, P.K., E. Casillas and U. Varanasi. 1997. Cytochemical responses in the digestive tissue of Mytilus edulis complex exposed to microencapsulated PAHs or PCBs. Comp. Biochem. Physiol. C, 118, 11-18
  24. Krishnakumar, P.K., P.K. Asokan and V.K. Pillai. 1990. Physiological and cellular responses to copper and mercury in the green mussel Perna viridis (Linnaeus). Aquat. Toxicol., 18, 1630-174
  25. Lagadic, L., T. Caquet and F. et Ramade. 1994. The role of biomarkers in environmental assessment. Invertebrate population and communities. Ecotoxicology., 3, 193-208 https://doi.org/10.1007/BF00117084
  26. Lomovasky, B.J., E. Morriconi, T. Brey and J. Calvo. 2002. Individual age and connective tissue lipofuscin in the hard clam Eurhomalea exalbida. J. Exp. Mar. Biol. Ecol., 276, 83-94 https://doi.org/10.1016/S0022-0981(02)00240-X
  27. Martel L., M.J. Gagnon, R. Masse, A. Leclerc and L. Tremblay. 1986. Polycyclic aromatic hydrocarbons in sediments from the Saguenay Fjord; Canada. Bull. Environ. Contam. Toxicol., 37, 133-140 https://doi.org/10.1007/BF01607740
  28. Matozzo, V. and M.G. Marin. 2005. Can 4-nonylphenol induce vitellogenin-like proteins in the clam Tapes philippinarum? Environ. Res., 97, 43-49 https://doi.org/10.1016/j.envres.2004.03.002
  29. Mori, K., T. Muramatsu and Y. Nakamura. 1969. Effect of steroid on oyster. III. Sex reversal from male to female in Crassostrea gigas by estradiol-17$\beta$. Bull. Jap. Soc. Sci. Fish., 35, 1072-1076 https://doi.org/10.2331/suisan.35.1072
  30. Munkittrick, K.R. and G. Van Der Kraak. 1999. Appropriate uses of physiological techniques for endocrine studies. In: Standardization of Biomarkers for Endocrine Disruption and Environmetntal Assessment, 8th Vol., Environmental Toxicology and Risk Assessment, Henshel, D.S., M.C. Black and M.C. Harrass, eds. ASTM, West Conshohocken, pp. 95-118
  31. Nimrod, A.C. and W.H. Benson. 1998. Reproduction and development of Japanese medaka following an early life stage exposure to xenoestrogens. Aquat. Toxicol., 44, 141-156 https://doi.org/10.1016/S0166-445X(98)00062-9
  32. Park, J.J., J.Y. Lee, J.S. Lee and Y.J. Chang. 2003. Gonadal development and gametogenic cycle of the equilateral venus, Gomphina veneriformis (Bivalvia: Veneridae). J. Kor. Fish. Soc., 36, 352-357
  33. Pickford, K.A., E. Thomas-Jones, B. Wheals, C.R. Tyler and J.P. Sumpter. 2003. Route of exposure affects the oestrogenic response of fish to 4-tert-nonylphenol. Aquat. Toxicol., 65, 267-279 https://doi.org/10.1016/S0166-445X(03)00149-8
  34. Quinn, B., F. Gagne, C. Blaise, M.J. Costello, J.G. Wilson and C. Mothersill. 2006. Evaluation of the lethal the sub-lethal toxicity and potential endocrine disrupting effect of nonylphenol on the zebra mussel (Dreissena polymorpha). Comp. Biochem. Physiol. C, 142, 118-127
  35. Quinn, B., F. Gagne, M. Costello, C. McKenzie, J. Wilson and C. Mothersill. 2004. The endocrine disrupting effect of municipal effluent on the zebra mussel (Dreissena polymorpha). Aquat. Toxicol., 66, 297-292
  36. Sarasquete, M.C., M.L. Gonzales de Canales and S. Gimeno. 1992. Comparative histopathological alterations in the digestive gland of marine bivalves exposed to Cu and Cd. Eur. J. Histochem., 36, 223-232
  37. Servos, M.R. 1999. Review of the aquatic toxicity, estrogenic responses and bioaccumulation of alkylphenols and alkylphenol polyethoxylates. Water Qual. Res. J. Can., 34, 123-177
  38. Siah, A., J. Pellerin, J.-C. Amiard, E. Pelletier and L. Viglino. 2003. Delayed gametogenesis and progesterone levels in soft-shell clams (Mya arenaria) in relation to in situ contamination to organotins and heavy metals in the St. Lawrence River (Canada). Camp. Biochem. Physiol. C, 135, 145-156
  39. Soto, A.M., H. Justicia, J.W. Wray and C. Sonnenschein. 1991. p-Nonylphenol: An estrogenic xenobiotic released from modified polystyrene. Environ. Health Perspect. 92, 167-173 https://doi.org/10.2307/3431154
  40. Sukhotin, A.A, D. Abele and H.O. Portner. 2002. Growth, metabolism and lipid peroxidation in Mytilus edulis L.: age and size effects. Mar. Ecol. Prog. Ser., 226, 223-234 https://doi.org/10.3354/meps226223
  41. Sundaram, K.M.S. and S. Szeto. 1981. The dissipation of nonylphenol in stream and pond water under simulated field conditions. J. Envrion. Sci. Health B, 16, 767-776 https://doi.org/10.1080/03601238109372293
  42. Tanghe, T., G. Devriese and W. Verstraeta. 1999. Nonylphenol and estrogenic activity in aquatic environmental samples. J. Environ. Qual., 28, 702-707 https://doi.org/10.2134/jeq1999.00472425002800020039x
  43. Tolls, J., P. Kloepper-Sams and D.T.H.M. Sijm. 1994. Surfactant bioconcentration - A critical review. Chemosphere, 29, 693-719 https://doi.org/10.1016/0045-6535(94)90040-X
  44. Viarengo, A., L. Canesi, M. Pertica, G. Poli, M.N. Moore and M. Orunesu. 1990. Heavy metal effects on lipid peroxidation in the tissues of Mytilus galloprovincialis Lam. Comp. Biochem. Physiol. C, 97, 37-42 https://doi.org/10.1016/0742-8413(90)90168-9
  45. Zorita, I., M. Ortiz-Zarragoitia, M. Soto and M.P. Cajaraville. 2006. Biomarkers in mussels from a copper site gradient (Visnes, Norway): An integrated biochemical, histochemical and histological study. Aquat, Toxicol., 78S, S109-S116

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