Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Selection of High Anthocyanin-Producing Cells from Habituated Callus Derived from Purple Sweetpotato

자색고구마 습관성캘러스로부터 안토시아닌 고생합성 세포주 선발

  • Park, Hye-Jeong (Department of Biology, College of Natural Science, Chosun University) ;
  • Kim, Yoon-Sil (Department of Biology, College of Natural Science, Chosun University) ;
  • Park, Hyeon-Yong (Department of Biology, College of Natural Science, Chosun University)
  • 박혜정 (조선대학교 자연과학대학 생물학과) ;
  • 김윤실 (조선대학교 자연과학대학 생물학과) ;
  • 박현용 (조선대학교 자연과학대학 생물학과)
  • Published : 2007.03.31
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Abstract

Callus induction using leaf of purple sweetpotato (PSP) was decreased when subcultured. So we selected habituated callus in MS medium supplemented with $1{\mu}M\;2,4-D$ (2,4-dichlorophenoxyacetic acid) after 6 months of cultures (without subculture). It grew faster and easier than any other callus. It was able to proliferate in MS hormone free solid and liquid medium without any growth regulators and subculture limits. During subculture in liquid medium, a purple mottled spot formed in one of habituated cell aggregates without any treatment. This purple cell aggregates were carefully separated from habituated cell aggregates, and then subcultured by selecting purple cell aggregates for more than 2 years to be isolated. The color value of the pigment extracted of culture was 1.0 mg/mL, which was close to that of a pigment extracted from storage root, which was 1.5 mg/mL. This purple cell aggregates could therefore be used for the industrial mass production of anthocyanin.

자색고구마 (Ipomoea batatas cv. Jami) 잎 조직배양으로 유도된 캘러스는 계대배양시 생장량이 감소하였다. 빠르고 간편하게 생장하는 습관성캘러스를 $1{\mu}M\;2,4-D$ 첨가된 MSBM에서 계대배양 없이 6개월간 지속배양하여 선발하였다. 습관성캘러스는 생장조절제가 첨가되지 않는 MSBM의 고체, 액체 배지에서 계대배양 횟수의 제한 없이 빠르게 지속생장이 가능하였다. 암조건의 액체배양 6 개월 후에는 자색생합성 부위가 생겼으며, 이를 2년 여간 분리하여 자색습관성세포괴를 선발하였다. 안토시아닌 함량은 자미의 괴근에서 추출되는 1.5 mg/ml에 근접한 1.0 mg/ml으로 나타났다. 자색습관성 세포괴는 안토시아닌 기내대량에 효과적 세포주로 사용 될 수 있을 것으로 기대한다.

Keywords

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