Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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Effect of Dietary Supplementation of Ground Grape Seed on Growth Performance and Antioxidant Status in the Intestine and Liver in Broiler Chickens

천연 항산화제로서 포도씨 분말 첨가가 육용계의 성장 및 항산화 작용에 미치는 영향

  • Jang, I.S. (Department of Animal Science & Biotechnology, RAIC, Jinju National University) ;
  • Ko, Y.H. (Department of Animal Science & Biotechnology, RAIC, Jinju National University) ;
  • Kang, S.Y. (Department of Animal Science & Biotechnology, RAIC, Jinju National University) ;
  • Moon, Y.S. (Department of Animal Science & Biotechnology, RAIC, Jinju National University) ;
  • Shon, S.H. (Department of Animal Science & Biotechnology, RAIC, Jinju National University)
  • 장인석 (진주산업대학교 동물생명과학과.동물생명산업지역협력연구센터) ;
  • 고영현 (진주산업대학교 동물생명과학과.동물생명산업지역협력연구센터) ;
  • 강선영 (진주산업대학교 동물생명과학과.동물생명산업지역협력연구센터) ;
  • 문양수 (진주산업대학교 동물생명과학과.동물생명산업지역협력연구센터) ;
  • 손시환 (진주산업대학교 동물생명과학과.동물생명산업지역협력연구센터)
  • Published : 2007.03.31
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Abstract

A total of twenty, 2-wk-old male broiler chickens were allotted into control diet(CON) or a diet supplemented with 1% ground grape seed(GGS). They had free access to feed and water for 3 wk. Growth performance and antioxidant markers in plasma, intestine and liver were determined. Dietary addition of 1% GGS did not affect weight gain, feed intake, feed conversion and organ weight in 35 day-old broiler chickens significantly. There was no difference in plasma levels of glucose, triglyceride, cholesterol, AST, ALT and LDH activity. However, total antioxidant status(TAS) in blood increased(P<0.05) in chickens fed the diet supplemented with 1% GGS compared to those fed the control diet. In addition, the specific activity of intestinal superoxide dismutase(SOD) increased(P<0.05) in birds fed the diet supplemented with GGS. However, the activities of intestinal gluthathione peroxidase(GSHPx) and gluthathione -S- transferase(GST) and hepatic SOD, GSHPx and GST were not affected by the dietary GGS. The levels of reduced glutathione and lipid peroxidation in the small intestine and liver were not different between the two groups. In conclusion, dietary supplementation of 1% GGS did not result in a negative effect on growth performance. In addition, some antioxidant indicators including blood TAS and intestinal SOD were markedly elevated in response to dietary GGS. Therefore, dietary addition of 1% GGS may be beneficial to improve antioxidant capacity in broiler chicken.

본 연구는 포도씨 분말 첨가 사료가 육계의 성장, 사료 이용성 및 체 조직내 항산화 효소 및 관련 생화학 지표 인자에 미치는 영향을 조사하기 위하여 수행되었다. 전체 50수 3일령 육계를 2주간 예비 실험 기간을 거친 다음 각 군당 10수씩 선발하여 대조구 및 포도씨 분말 1% 첨가구 등 2개 구를 설정하여 3주간 사양 시험을 실시하고 혈액 생화학 성분 및 체조직의 항산화 지표를 분석하였다. 그 결과는 다음과 같다. 1. 육계 사료내 1% 수준의 포도씨 분말 첨가는 체중, 일당 증체량, 사료 섭취량, 사료 요구율 및 장기 무게 등에 유의적인 영향을 미치지 않았다. 2. 혈액내 total antioxidant status(TAS)는 포도씨 첨가구에서 유의적(P<0.05)으로 증가하였으며, 혈중 생화학적 성분과 조직 손상을 나타내는 AST, ALT, LDH 등의 수준은 모든 구에서 비슷한 수준을 보였다. 3. 포도씨 분말 첨가는 소장 점막 세포내 SOD 활성도를 유의적으로(P<0.05) 증가시켰다. 그러나 간 조직내 GSHPx, SOD, GST 등과 같은 항산화 효소, 환원형 glutathione 및 지질 과산화(MDA) 수준은 대조구 및 포도씨 분말 급여구간에 차이는 없었다. 이상의 결과를 종합해 볼 때 천연 항산화제로서 포도씨 분말 1% 첨가 사료는 성장 및 사료 이용성에 특이적 영향을 미치지 않았으나, 혈액내 TAS 및 소장내 SOD활성도를 현저히 증가시키므로 육계에서 천연 항산화원으로서의 사용이 가능할 것으로 사료된다.

Keywords

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