Journal of Biosystems Engineering

Korean Society for Agricultural Machinery (한국농업기계학회)
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Biomechanical Properties and Cytotoxicity of Chitosan Patch Scaffold for Artificial Eardrum

인조고막용 키토산 패치 지지체의 생체역학적 특성 및 독성 평가

  • Chung, Jong-Hoon (Dept. of Biosystems & Biomaterials Science and Engineering, Seoul National University) ;
  • Kim, Jang-Ho (Dept. of Biosystems & Biomaterials Science and Engineering, Seoul National University) ;
  • Choung, Yun-Hoon (Dept. of Otolaryngology, Ajou University School of Medicine) ;
  • Im, Ae-Lee (Dept. of Biosystems & Biomaterials Science and Engineering, Seoul National University) ;
  • Lim, Ki-Taek (Dept. of Biosystems & Biomaterials Science and Engineering, Seoul National University) ;
  • Hong, Ji-Hyang (Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Choung, Pill-Hoon (Dept. of Oral and Maxillofacial Surgery, Tooth Bioengineering National Research Lab, School of Dentistry, Seoul National University)
  • Published : 2007.02.25
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Abstract

The objectives of this study were to prepare a new artificial eardrum patch using water-insoluble chitosan for healing the tympanic membrane perforations and to investigate biomechanical properties and cyotoxicity of the chitosan patch scaffold (CPS). Tensile strength and elongation at the rupture point of CPSs were 2.49-74.05 MPa and 0.11-107.06%, respectively. As the biomechanical properties or CPSs varied with the concentration of chitosan and glycerol, the proper conditions for the CPS were found out. SEM analysis showed very smooth and uniform surface of CPSs without pores at x1000. The result of MTT test showed that CPSs had no cytotoxicity.

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References

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  1. Regeneration of Chronic Tympanic Membrane Perforation Using an EGF-Releasing Chitosan Patch vol.19, pp.17-18, 2013, https://doi.org/10.1089/ten.tea.2012.0617
  2. Regeneration of chronic tympanic membrane perforation using 3D collagen with topical umbilical cord serum vol.62, 2013, https://doi.org/10.1016/j.ijbiomac.2013.08.049