Journal of Biosystems Engineering

Korean Society for Agricultural Machinery (한국농업기계학회)
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Preparation and Biocompatibility of Composite Bone Scaffolds Using Gnotobiotic Pig Bones

무균돼지뼈를 이용한 복합 골지지체의 제조와 생체적합성 평가

  • Im, Ae-Lee (Dept. of Biosystems & Biomaterials Science and Engineering, Seoul National University) ;
  • Chung, Jong-Hoon (Dept. of Biosystems & Biomaterials Science and Engineering, Seoul National University) ;
  • Lim, Ki-Taek (Dept. of Biosystems & Biomaterials Science and Engineering, Seoul National University) ;
  • Choung, Pill-Hoon (Tooth Bioengineering National Research Lab., Dept. of Oral and Maxillofacial Surgery, College of Dentistry) ;
  • Hong, Ji-Hyang (Research Institute for Agriculture and Life Sciences, Seoul National University)
  • Published : 2007.02.25
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Abstract

Highly porous composite bioceramic bone scaffolds were developed using sintered gnotobiotic pig bones. These scaffolds consisted of poly-D,L-lactic acid (P(D,L)LA) and bioceramic materials of pig bone powder. The bone scaffolds were able to promote biocompatibility and possess interconnected pores that would support cell adhesion and proliferation adequately. The composite scaffolds were tested with dental pulp stem cells for cytotoxicity test. Cells seeded on the composite scaffolds were readily attached, well proliferated, as confirmed by cytotoxicity test, and cell adhesion assessment. The composite bone scaffold had no toxicity in cytotoxicity test on the extract of 0.013 g scaffold to 2 ml culture medium. The cells on the composite bone scaffold proliferated better than cells on the P(D,L)LA scaffolds.

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