Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Comparison of Extraction Methods of Chondroitin Sulfate from Meat By-products

식육부산물에서의 Chondroitin Sulfate 추출방법간의 비교

  • Lim, D.G. (Department of Animal Science and Biotechnology, Seoul National University) ;
  • Oh, D.H. (Department of Animal Science and Biotechnology, Seoul National University) ;
  • Seol, K.H. (Department of Animal Science and Biotechnology, Seoul National University) ;
  • Lee, Moo-Ha (Department of Animal Science and Biotechnology, Seoul National University)
  • 임동균 (서울대학교 동물생명공학전공) ;
  • 오동훈 (서울대학교 동물생명공학전공) ;
  • 설국환 (서울대학교 동물생명공학전공) ;
  • 이무하 (서울대학교 동물생명공학전공)
  • Published : 2007.08.31
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Abstract

A total of 127 Korean native black pigs(gilt 30, boar 97) were used to investigate the carcass properties, yields of retail cut and meat quality. The average live weight, chilled carcass percentage and retail cut percentage used in this study were 74.69kg, 71.14% and 61.09% for boar, and 75.5kg, 73.88% and 56.25% for gilt, respectively. Although gilts were higher in live weight and chilled carcass percentage whereas lower in retail cut percentage when compared to boars, there was no significantly difference between gender(p<0.05). Gilts had higher fat contents than boars regardless of weight range groups. Protein contents increased as slaughter weight increased for gilt and boar groups. Gilts contained high intramuscular fat contents had significantly lower Warner-Bratzler shear force(WBS) and water holding capacity(WHC) when compared to boar in the same weight range group(p<0.05). There was no significantly different in meat color(p>0.05) although gilts had higher L(lightness), a(redness) and b(yellowness) values when compared to the same group of boar. From the result of this study can be used for the grading guideline and quality evaluation standard of Korean native black pigs by gender and live weight.

본 연구는 소, 돼지, 닭의 부산물인 신장, 간, 기관으로부터 황산콘드로이틴을 추출하고 추출수율을 극대화할 수 있는 추출조건을 탐색하고자 실시하였다. 추출방법은 일반추출방법, 열수추출방법 및 효소가수분해 추출방법을 이용하였으며 각 추출방법에 따른 수율 및 추출물내의 황산 콘드로이틴의 양을 측정하였다. 추출방법간의 비교에서 일반추출법(대조구) 보다 열수추출과 효소가수분해 추출의 수율이 더 높게 나타났으며 유의적인 차이를 보였다(P<0.05). 효소가수분해 추출에서 식육부산물 중 가장 높은 값을 나타낸 것은 닭 기관, 소 기관, 소 간 순이었다(P<0.05). 모든 추출방법 간에 있어서 소기관의 황산콘드로이틴 함량이 가장 높았으며 효소가수분해 추출방법에 의한 소기관의 황산콘드로이틴 함량이(21.19±1.82) 가장 높은 수치를 나타내었다(P<0.05). 추출물의 수율에 영향을 미치는 에탄올에 의한 추출물 분별침전에서는 에탄올 농도가 증가할수록 황산콘드로이틴 수율이 증가하였다. 결론적으로 식육부산물로부터 황산콘드로이틴을 추출하는데 있어 효소가수분해방법이 가장 좋은 결과를 나타내었다.

Keywords

References

  1. AOAC. 1995. Official methods of analysis. 16th ed, Association Official Analytical Chemists, Washington, DC
  2. Asher, R. A., Morgenstern, D. A., Moon, L. D. F. and Fawcett, J. W. 2001. Chondroitin sulfate proteoglycans:inhibitory components of the glial scar. Progress in Brain Research. 132:611-619 https://doi.org/10.1016/S0079-6123(01)32106-4
  3. Dietrich, C. P., Sampaio, L. O. and Olga, M. S. 1976. Characteristic distribution of sulfated mucopolysaccharides in different tissues and in their respective mitochondria. Biochemical and Biophysical Research Communications. 71:1-10 https://doi.org/10.1016/0006-291X(76)90241-2
  4. Gamjanagoonchorn, W., Wongekalak, L. and Engkagul, A 2007. Determination of chodroitin sulfate from different sources of cartilage. Chemical Engineering. Proc. 46:465-471
  5. Hui, Y. H., Nip, W. K., Rogers, R. W. and Young, O. A. 2001. Meat science and applications. Marcel Dekker, Inc., New York
  6. Hungerford, D. 2003. The role of Chondroitin sulfate in the management of osteoarthritis. //www.slackinc.com/bone/ortoday/supp/0603/symposi um.htm
  7. Hungerford, D. and Jones, L. C. 2003. Glucosamine and Chondroitin sulfate are effective in the management of osteoarthritis. The Journal of arthroplasty 18(3):1
  8. Jo, J. H., Do, J. R., Kim, Y. M., Kim, D. S., Lee, T. K., Kim, S. B., Cho, S. M., Kang, S. N. and Park, D. C. 2005. Optimization of shark cartilage hydrolysis for the preparation of Chondroitin sulfate. Food sci. biotechnol. 14(5): 651-655
  9. Kitagawa, H., Tanaka, Y., Tsuchida, K.. Goto, F., Ogawa, T., Lidholt, K, Lindahl, U. and Sugahara K 1995. N-acetylgalactosamine transfer to the common carbohydrate- protein Inkage region of sulfated glycosaminoglycans-identification of UDPGaINAc: chodro-oligosaccharide a N-acetylgalacto-saminyltransferase in fetal bovine serum. J. Bio. Chem. 270:22190-22195 https://doi.org/10.1074/jbc.270.38.22190
  10. Lignot, B, Lahogue, V. and Bourseau, P. 2003. Enzymatic extraction of Chondroitin sulfate from skate cartilage and concentration-desalting by ultrafiltration. Journal of biotechnology 103:281-284 https://doi.org/10.1016/S0168-1656(03)00139-1
  11. McAlindon., T. E., Lavalley, M. P., Gulin, J. P. and Felson, D. T. 2000. Glucosamine and Chondroitin for treatment of osteoarthritis; a systematic quality assessment and meta-analysis, J. Am. Med. Assoc. 283:1469-1475 https://doi.org/10.1001/jama.283.11.1469
  12. Mourao, P. A. S., Boisson-Vidal, C., TaponBretaudiere, J. Drouet, B,. Fischer A. B. and Marie, A 2001. Inactivation of Thrombin by a Fucosylated Chondroitin Sulfate from Echinoderm. Thrombosis Research. 102:167-176 https://doi.org/10.1016/S0049-3848(01)00230-4
  13. Mourao, P. A. S. and Dietrich, C. P. 1979. Chondroitin sulfates of the epiphysial cartilages of different mammals. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 62:115-117 https://doi.org/10.1016/0305-0491(79)90023-3
  14. Park, D. C., Gu, Y. S., Ji, C. I. and Kim, S. B. 2001. Enzymatic hydrolysis conditions for preparation of sea cucumber hydrolysates containing Chondroitin Sulfate. Food sci. biotechnol. 10(6): 686-689
  15. Rivera, J. A., Sebranek, J. G., Rust, R E. and Tabatabai, L. B. 2000. Composition and protein fractions of different meat by-products used for petfood compared with mechanically separated chicken. Meat sci. 55:53-59 https://doi.org/10.1016/S0309-1740(99)00125-4
  16. SAS. 2003. SAS/STAT Software for rc Release 6.11, SAS Institute Inc., Cary, NC, USA
  17. Sanne, V., Larnkjoer, A., 0stergaard, P., Nielsen, J. I. and Nordfang, O. 1994. Characterization of the binding between tissue factor pathway inhibitor and glycosaminoglycans. Thrombosis Research. 75:173-183 https://doi.org/10.1016/0049-3848(94)90066-3
  18. Toledo, M. Olga, M. S. and Dietrich, C. P. 1977. Tissue specific disaibution of sulfated mucopolysaccharides in mammals. Biochimica et Biophysica Acta (BBA) - General Subjects. 498:114-122 https://doi.org/10.1016/0304-4165(77)90092-7
  19. Volpi, N., Rita, F. and Venturelli, T. 1995. Activity of chondroitin ABC lyase on dennatan sulfate partially degraded by cupric-ion-mediated free-radical treatment. J. Chromatography B: Biorned. Sci. and Applications. 669:197-205 https://doi.org/10.1016/0378-4347(95)00108-U
  20. 유익종, 김경환, 류화정. 1995. 쇠고기 열수추출 잔사를 이용한 산 가수분해물의 생산조건에 관 한 연구. Korean J. Food Sci. Ani. Resour. 15(1):58-62
  21. 이영근, 강정미. 1994. Spectrophotometer 및 HPLC에 의한 식용달팽이의 황산콘드로이틴 분 석. 한국영양식량학회. 23(6):945-949
  22. 한국식품공전. 2001. Mucopolysaccharide protein food. Korean food industry association. p. 352-355
  23. Meat Journal. 2007. 3월호 국내통계