Journal of Marine Bioscience and Biotechnology (한국해양바이오학회지)

The Korean Society for Marine Biotechnology (한국해양바이오학회)
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Gene Expression Profiles in the Peripheral Blood Leukocytes (PBL) of Olive Flounder Paralichthys olivaceus after Stimulation with Lipoolysaccharide (LPS) in vitro

지질다당질로 자극한 넙치 백혈구의 발현유전자의 분석

  • Nam, Bo-Hye (Biotechnology Research Institute, National Fisheries Research & Development Institute) ;
  • Moon, Ji-Young (Biotechnology Research Institute, National Fisheries Research & Development Institute) ;
  • Kim, Young-Ok (Biotechnology Research Institute, National Fisheries Research & Development Institute) ;
  • Kim, Woo Jin (Biotechnology Research Institute, National Fisheries Research & Development Institute) ;
  • Kong, Hee Jeong (Biotechnology Research Institute, National Fisheries Research & Development Institute) ;
  • Lee, Sang-Jun (Biotechnology Research Institute, National Fisheries Research & Development Institute) ;
  • Choi, Tae-Jin (Biotechnology Research Institute, National Fisheries Research & Development Institute)
  • 남보혜 (국립수산과학원 생명공학연구소) ;
  • 문지영 (국립수산과학원 생명공학연구소) ;
  • 김영옥 (국립수산과학원 생명공학연구소) ;
  • 김우진 (국립수산과학원 생명공학연구소) ;
  • 공희정 (국립수산과학원 생명공학연구소) ;
  • 이상준 (국립수산과학원 생명공학연구소) ;
  • 최태진 (국립수산과학원 생명공학연구소)
  • Published : 2007.09.30
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Abstract

Suppression subtractive hybridization (SSH) was performed to construct an cDNA library of olive flounder Paralichthys olivaceus peripheral blood leukocytes (PBL) stimulated with lipopolysaccharide (LPS). Total 470 clones were randomly selected and sequenced, 214 out of 470 sequences showed identities with known genes and 252 sequences were unknown genes. Among the 218 known genes, 34 sequences were found to be homologous to IL-1RII gene, and 14 sequences were identified as IL-$1{\beta}$. RT-PCR analysis showed that IL-$1{\beta}$ mRNA was induced 1h after LPS-stimulation, and IL-1RII was increased from 3h after stimulation, indicating that most of SSH clones are associated with inflammatory responses in fish, and this SSH cDNA library would be useful to identify bio-defense and immuno-related genes in fish.

넙치의 생체방어 및 면역관련 유전자의 대량확보를 위하여, 지질다당질로 자극한 넙치의 말초백혈구 cDNA library를 suppression subtractive hybridization 방법으로 제작하여 발현유전자의 발현 양상 및 특성을 분석하였다. 총 470개의 SSH 클론 중 기존에 밝혀진 유전자와 상동성을 보인 218개의 클론을 분석한 결과, 대표적인 전염증사이토카인 IL-1의 유인수용체인 IL-1RII가 34번의 가장 높은 빈도로 발현되었으며, IL-$1{\beta}$가 14번의 높은 발현 빈도를 보였다. 그 중 펩티도글리칸 인식 단백질및 혈관활성 장 펩타이드 유전자는 어류에서는 처음으로 보고되는 것으로, 지질다당질로 자극한 넙치의 말초백혈구 SSH cDNA library는 다양한 생체방어 및 면역관련 유전자를 포함하고 있었다.

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